Composition and diversity of prokaryotes at an iron ore post‐mining site revealed the natural resilience 10years after mining exploitation

Prokaryotes play crucial roles in the rehabilitation process to restore the ecological integrity of disturbed areas. This work reports on the profiles of N‐fixing microorganisms and Actinobacteria, from DGGE, the prokaryotic composition from next‐generation sequencing (NGS) and physicochemical soil characteristics. We compared a deactivated mining site where mining ended about 10 years ago, when the revegetation process was begun (RV), and a reference site, with natural vegetation (NT), both located at Retiro das Almas Mine, in the Quadrilátero Ferrífero region, Minas Gerais, Brazil. In both sites, the most abundant archaeal and bacterial groups included Euryarchaeota, Thaumarchaeota, Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia and revealed differences in their ecological metrics and distribution. Proteobacteria and Actinobacteria were most abundant in RV sites, while Acidobacteria and Verrucomicrobia were most abundant in NT sites. Less abundant phyla, such as Bathyarchaeota (Archaea) and GAL 15 (Bacteria) were found only in NT, while Gracilibacteria, Ignavibacteriae BJ‐169, and BRC1 were only found in RV. The majority of identified bacterial genera were shared by RV and NT. Soil P, pH, and particle density were most significant (p