Genetic polymorphisms in XRCC1, OGG1, and XRCC3 DNA repair genes and DNA damage in radiotherapy workers
DNA damage may develop at any dose of ionizing radiation. DNA damage activates pathways that regulate cell growth and division or coordinate its replication and repair. The repair pathways, base excision repair (BER) and single-strand break repair (SSBR), can repair such damages efficiently and main...
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| description | DNA damage may develop at any dose of ionizing radiation. DNA damage activates pathways that regulate cell growth and division or coordinate its replication and repair. The repair pathways, base excision repair (BER) and single-strand break repair (SSBR), can repair such damages efficiently and maintain genome integrity. Loss of this repair process or alteration of its control will be associated with serious outcomes for cells and individuals. This study aimed to determine the relationship between XRCC1 (Arg194Trp, Arg280His, and Arg399Gln), OGG1 (Ser326Cys), and XRCC3 (Thr241Met) SNPs and DNA damage and to identify high-risk individuals with reduced DNA repair capacity. This case-control study was conducted on 80 subjects; 50 subjects working in Clinical Oncology and Nuclear Medicine Department in Assiut University Hospital along with 30 controls. A total of 1 mL blood samples were collected for Single-Cell Gel Electrophoresis Technique (Comet Assay) for detection of DNA damage in those subjects. A total of 3 mL fresh blood samples were collected and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)–based technique. DNA damage detected by comet test was significantly high in IR-exposed workers than control. Statistically high significant difference was found in exposed subjects versus control subjects regarding the frequencies of the variant alleles of hOGG1
326
, XRCC1
280 & 399
, and XRCC3
241
. The level of DNA damage was not affected by OGG1
326
SNPs when comparing subjects of wild genotype with those of (pooled) variants either in the exposed staff or in the control group while XRCC1
280, 399
and XRCC3
241
variant alleles had an influence on the studied DNA damage biomarker. Moreover, genotyping distribution pattern was highly variable in relation to gender. The present study indicated a relationship between DNA damage detected by comet test and single nucleotide polymorphisms in genes coding for DNA certain repair enzymes. Individuals occupationally exposed to low doses of ionizing radiation could be at great risk and more susceptible to the increased DNA damage if they have inherited genetic polymorphism. |
| doi_str_mv | 10.1007/s11356-020-10270-9 |
| format | Article |
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326
, XRCC1
280 & 399
, and XRCC3
241
. The level of DNA damage was not affected by OGG1
326
SNPs when comparing subjects of wild genotype with those of (pooled) variants either in the exposed staff or in the control group while XRCC1
280, 399
and XRCC3
241
variant alleles had an influence on the studied DNA damage biomarker. Moreover, genotyping distribution pattern was highly variable in relation to gender. The present study indicated a relationship between DNA damage detected by comet test and single nucleotide polymorphisms in genes coding for DNA certain repair enzymes. Individuals occupationally exposed to low doses of ionizing radiation could be at great risk and more susceptible to the increased DNA damage if they have inherited genetic polymorphism.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-020-10270-9</identifier><identifier>PMID: 32740834</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alleles ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Base excision repair ; Bioassays ; Biomarkers ; Blood ; Case-Control Studies ; Comet assay ; Damage detection ; Deoxyribonucleic acid ; DNA ; DNA Damage ; DNA Glycosylases - genetics ; DNA repair ; DNA Repair - genetics ; Earth and Environmental Science ; Ecotoxicology ; Electrophoresis ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Exposure ; Gel electrophoresis ; Gene polymorphism ; Genes ; Genomes ; Genotype ; Genotyping ; Humans ; Ionizing radiation ; Nuclear medicine ; Nucleotides ; Occupational exposure ; OGG1 protein ; Oncology ; Polymerase chain reaction ; Polymorphism ; Polymorphism, Single Nucleotide ; Radiation ; Radiation damage ; Radiation dosage ; Radiation therapy ; Repair ; Research Article ; Restriction fragment length polymorphism ; Single-nucleotide polymorphism ; Statistical analysis ; Statistical methods ; Waste Water Technology ; Water Management ; Water Pollution Control ; X-ray Repair Cross Complementing Protein 1 - genetics ; XRCC1 protein</subject><ispartof>Environmental science and pollution research international, 2020-12, Vol.27 (35), p.43786-43799</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-2d4fab8e35b8aa1d13526a6b52164869d0c84cdb27c71e4968e559b4dc8030533</citedby><cites>FETCH-LOGICAL-c412t-2d4fab8e35b8aa1d13526a6b52164869d0c84cdb27c71e4968e559b4dc8030533</cites><orcidid>0000-0001-5995-9177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-020-10270-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-020-10270-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32740834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soliman, Asmaa H. M.</creatorcontrib><creatorcontrib>Zaki, Nasef N.</creatorcontrib><creatorcontrib>Fathy, Hala M.</creatorcontrib><creatorcontrib>Mohamed, Aml A.</creatorcontrib><creatorcontrib>Ezzat, Mohamed A.</creatorcontrib><creatorcontrib>Rayan, Amal</creatorcontrib><title>Genetic polymorphisms in XRCC1, OGG1, and XRCC3 DNA repair genes and DNA damage in radiotherapy workers</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>DNA damage may develop at any dose of ionizing radiation. DNA damage activates pathways that regulate cell growth and division or coordinate its replication and repair. The repair pathways, base excision repair (BER) and single-strand break repair (SSBR), can repair such damages efficiently and maintain genome integrity. Loss of this repair process or alteration of its control will be associated with serious outcomes for cells and individuals. This study aimed to determine the relationship between XRCC1 (Arg194Trp, Arg280His, and Arg399Gln), OGG1 (Ser326Cys), and XRCC3 (Thr241Met) SNPs and DNA damage and to identify high-risk individuals with reduced DNA repair capacity. This case-control study was conducted on 80 subjects; 50 subjects working in Clinical Oncology and Nuclear Medicine Department in Assiut University Hospital along with 30 controls. A total of 1 mL blood samples were collected for Single-Cell Gel Electrophoresis Technique (Comet Assay) for detection of DNA damage in those subjects. A total of 3 mL fresh blood samples were collected and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)–based technique. DNA damage detected by comet test was significantly high in IR-exposed workers than control. Statistically high significant difference was found in exposed subjects versus control subjects regarding the frequencies of the variant alleles of hOGG1
326
, XRCC1
280 & 399
, and XRCC3
241
. The level of DNA damage was not affected by OGG1
326
SNPs when comparing subjects of wild genotype with those of (pooled) variants either in the exposed staff or in the control group while XRCC1
280, 399
and XRCC3
241
variant alleles had an influence on the studied DNA damage biomarker. Moreover, genotyping distribution pattern was highly variable in relation to gender. The present study indicated a relationship between DNA damage detected by comet test and single nucleotide polymorphisms in genes coding for DNA certain repair enzymes. Individuals occupationally exposed to low doses of ionizing radiation could be at great risk and more susceptible to the increased DNA damage if they have inherited genetic polymorphism.</description><subject>Alleles</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Base excision repair</subject><subject>Bioassays</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>Case-Control Studies</subject><subject>Comet assay</subject><subject>Damage detection</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA Glycosylases - genetics</subject><subject>DNA repair</subject><subject>DNA Repair - genetics</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Electrophoresis</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Exposure</subject><subject>Gel electrophoresis</subject><subject>Gene polymorphism</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>Humans</subject><subject>Ionizing radiation</subject><subject>Nuclear medicine</subject><subject>Nucleotides</subject><subject>Occupational exposure</subject><subject>OGG1 protein</subject><subject>Oncology</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Radiation</subject><subject>Radiation damage</subject><subject>Radiation dosage</subject><subject>Radiation therapy</subject><subject>Repair</subject><subject>Research Article</subject><subject>Restriction fragment length polymorphism</subject><subject>Single-nucleotide polymorphism</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>X-ray Repair Cross Complementing Protein 1 - genetics</subject><subject>XRCC1 protein</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1LAzEQhoMotlb_gAdZ8Go0X5tsjrLqKhQLouAtZDfpdmv3w2SL9N-bdqvevMzAzPO-w7wAnGN0jRESNx5jGnOICIIYEYGgPABjzDGDgkl5CMZIMgYxZWwETrxfokBKIo7BiBLBUELZGJSZbWxfFVHXrjZ167pF5WsfVU30_pKm-CqaZVmoujG7AY3unm8jZztduagMUr9bbYdG17q0W6XTpmr7hXW620Rfrfuwzp-Co7leeXu27xPw9nD_mj7C6Sx7Sm-nsGCY9JAYNtd5YmmcJ1pjEx4kXPM8JpizhEuDioQVJieiENgyyRMbxzJnpkgQRTGlE3A5-Hau_Vxb36tlu3ZNOKkIE5SShFMRKDJQhWu9d3auOlfV2m0URmqbrRqyVSExtctWySC62Fuv89qaX8lPmAGgA-DDqimt-7v9j-03XIWBRw</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Soliman, Asmaa H. 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M. ; Zaki, Nasef N. ; Fathy, Hala M. ; Mohamed, Aml A. ; Ezzat, Mohamed A. ; Rayan, Amal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-2d4fab8e35b8aa1d13526a6b52164869d0c84cdb27c71e4968e559b4dc8030533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alleles</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Base excision repair</topic><topic>Bioassays</topic><topic>Biomarkers</topic><topic>Blood</topic><topic>Case-Control Studies</topic><topic>Comet assay</topic><topic>Damage detection</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA Glycosylases - genetics</topic><topic>DNA repair</topic><topic>DNA Repair - genetics</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Electrophoresis</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Exposure</topic><topic>Gel electrophoresis</topic><topic>Gene polymorphism</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>Humans</topic><topic>Ionizing radiation</topic><topic>Nuclear medicine</topic><topic>Nucleotides</topic><topic>Occupational exposure</topic><topic>OGG1 protein</topic><topic>Oncology</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Radiation</topic><topic>Radiation damage</topic><topic>Radiation dosage</topic><topic>Radiation therapy</topic><topic>Repair</topic><topic>Research Article</topic><topic>Restriction fragment length polymorphism</topic><topic>Single-nucleotide polymorphism</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>X-ray Repair Cross Complementing Protein 1 - genetics</topic><topic>XRCC1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soliman, Asmaa H. M.</creatorcontrib><creatorcontrib>Zaki, Nasef N.</creatorcontrib><creatorcontrib>Fathy, Hala M.</creatorcontrib><creatorcontrib>Mohamed, Aml A.</creatorcontrib><creatorcontrib>Ezzat, Mohamed A.</creatorcontrib><creatorcontrib>Rayan, Amal</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soliman, Asmaa H. M.</au><au>Zaki, Nasef N.</au><au>Fathy, Hala M.</au><au>Mohamed, Aml A.</au><au>Ezzat, Mohamed A.</au><au>Rayan, Amal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic polymorphisms in XRCC1, OGG1, and XRCC3 DNA repair genes and DNA damage in radiotherapy workers</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>27</volume><issue>35</issue><spage>43786</spage><epage>43799</epage><pages>43786-43799</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>DNA damage may develop at any dose of ionizing radiation. DNA damage activates pathways that regulate cell growth and division or coordinate its replication and repair. The repair pathways, base excision repair (BER) and single-strand break repair (SSBR), can repair such damages efficiently and maintain genome integrity. Loss of this repair process or alteration of its control will be associated with serious outcomes for cells and individuals. This study aimed to determine the relationship between XRCC1 (Arg194Trp, Arg280His, and Arg399Gln), OGG1 (Ser326Cys), and XRCC3 (Thr241Met) SNPs and DNA damage and to identify high-risk individuals with reduced DNA repair capacity. This case-control study was conducted on 80 subjects; 50 subjects working in Clinical Oncology and Nuclear Medicine Department in Assiut University Hospital along with 30 controls. A total of 1 mL blood samples were collected for Single-Cell Gel Electrophoresis Technique (Comet Assay) for detection of DNA damage in those subjects. A total of 3 mL fresh blood samples were collected and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)–based technique. DNA damage detected by comet test was significantly high in IR-exposed workers than control. Statistically high significant difference was found in exposed subjects versus control subjects regarding the frequencies of the variant alleles of hOGG1
326
, XRCC1
280 & 399
, and XRCC3
241
. The level of DNA damage was not affected by OGG1
326
SNPs when comparing subjects of wild genotype with those of (pooled) variants either in the exposed staff or in the control group while XRCC1
280, 399
and XRCC3
241
variant alleles had an influence on the studied DNA damage biomarker. Moreover, genotyping distribution pattern was highly variable in relation to gender. The present study indicated a relationship between DNA damage detected by comet test and single nucleotide polymorphisms in genes coding for DNA certain repair enzymes. Individuals occupationally exposed to low doses of ionizing radiation could be at great risk and more susceptible to the increased DNA damage if they have inherited genetic polymorphism.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32740834</pmid><doi>10.1007/s11356-020-10270-9</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-5995-9177</orcidid></addata></record> |
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| issn | 0944-1344 1614-7499 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Alleles Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Base excision repair Bioassays Biomarkers Blood Case-Control Studies Comet assay Damage detection Deoxyribonucleic acid DNA DNA Damage DNA Glycosylases - genetics DNA repair DNA Repair - genetics Earth and Environmental Science Ecotoxicology Electrophoresis Environment Environmental Chemistry Environmental Health Environmental science Exposure Gel electrophoresis Gene polymorphism Genes Genomes Genotype Genotyping Humans Ionizing radiation Nuclear medicine Nucleotides Occupational exposure OGG1 protein Oncology Polymerase chain reaction Polymorphism Polymorphism, Single Nucleotide Radiation Radiation damage Radiation dosage Radiation therapy Repair Research Article Restriction fragment length polymorphism Single-nucleotide polymorphism Statistical analysis Statistical methods Waste Water Technology Water Management Water Pollution Control X-ray Repair Cross Complementing Protein 1 - genetics XRCC1 protein |
| title | Genetic polymorphisms in XRCC1, OGG1, and XRCC3 DNA repair genes and DNA damage in radiotherapy workers |
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