Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana
BACKGROUND Ultrasound (US) treatment was applied to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the paramet...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 2021-01, Vol.96 (1), p.88-98 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 96 |
| creator | Schmaltz, Silvana Aita, Bruno C Alves, Eliana A Fochi, Alex Bolson, Vinícius F Navarro‐Díaz, Helmut J Kuhn, Raquel C Mazutti, Marcio A |
| description | BACKGROUND
Ultrasound (US) treatment was applied to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the parameters evaluated.
RESULTS
The best strategy comprised ultrasonication during 5 min at 24 h of fermentation with a power output of 195 W (fixed frequency of 24 kHz) and a duty cycle of 0.5 s.s−1 (0.5 s ON, 0.5 s OFF). The US treatment resulted in a production increase of 46% for β‐1,3‐glucanase and 42% for chitinase compared to the non‐sonicated control. Fragmentations of the mycelia in the sonicated samples were observed in Scanning Electron Microscopy (SEM) micrographs after the US application, supporting the idea of a better release of enzymes to outside the cell.
CONCLUSION
Ultrasound‐assisted fermentation was a promising tool to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66). The combination of US parameters showed to be decisive in obtaining higher enzyme production when compared to the control non‐sonicated experiment. Therefore, this work brings a significant contribution to the field of stimulation of bioprocesses with ultrasound since it shows a strategy to obtain higher enzyme production when compared to traditional fermentation. © 2020 Society of Chemical Industry |
| doi_str_mv | 10.1002/jctb.6514 |
| format | Article |
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Ultrasound (US) treatment was applied to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the parameters evaluated.
RESULTS
The best strategy comprised ultrasonication during 5 min at 24 h of fermentation with a power output of 195 W (fixed frequency of 24 kHz) and a duty cycle of 0.5 s.s−1 (0.5 s ON, 0.5 s OFF). The US treatment resulted in a production increase of 46% for β‐1,3‐glucanase and 42% for chitinase compared to the non‐sonicated control. Fragmentations of the mycelia in the sonicated samples were observed in Scanning Electron Microscopy (SEM) micrographs after the US application, supporting the idea of a better release of enzymes to outside the cell.
CONCLUSION
Ultrasound‐assisted fermentation was a promising tool to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66). The combination of US parameters showed to be decisive in obtaining higher enzyme production when compared to the control non‐sonicated experiment. Therefore, this work brings a significant contribution to the field of stimulation of bioprocesses with ultrasound since it shows a strategy to obtain higher enzyme production when compared to traditional fermentation. © 2020 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.6514</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Beauveria bassiana ; Chitinase ; Enzymes ; Fermentation ; Micrography ; Mycelia ; Parameters ; Photomicrographs ; Scanning electron microscopy ; submerged fermentation; chitinase; β‐1,3‐glucanase; process intensification; ultrasound stimulation ; Ultrasonic imaging ; Ultrasound</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2021-01, Vol.96 (1), p.88-98</ispartof><rights>2020 Society of Chemical Industry</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2974-3c8e64bb4944a94bc7829ef6f0f999e3eabd90ee02dd8fa6aae16e90ed97a7bc3</citedby><cites>FETCH-LOGICAL-c2974-3c8e64bb4944a94bc7829ef6f0f999e3eabd90ee02dd8fa6aae16e90ed97a7bc3</cites><orcidid>0000-0002-0175-044X ; 0000-0001-8217-5629</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjctb.6514$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjctb.6514$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Schmaltz, Silvana</creatorcontrib><creatorcontrib>Aita, Bruno C</creatorcontrib><creatorcontrib>Alves, Eliana A</creatorcontrib><creatorcontrib>Fochi, Alex</creatorcontrib><creatorcontrib>Bolson, Vinícius F</creatorcontrib><creatorcontrib>Navarro‐Díaz, Helmut J</creatorcontrib><creatorcontrib>Kuhn, Raquel C</creatorcontrib><creatorcontrib>Mazutti, Marcio A</creatorcontrib><title>Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana</title><title>Journal of chemical technology and biotechnology (1986)</title><description>BACKGROUND
Ultrasound (US) treatment was applied to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the parameters evaluated.
RESULTS
The best strategy comprised ultrasonication during 5 min at 24 h of fermentation with a power output of 195 W (fixed frequency of 24 kHz) and a duty cycle of 0.5 s.s−1 (0.5 s ON, 0.5 s OFF). The US treatment resulted in a production increase of 46% for β‐1,3‐glucanase and 42% for chitinase compared to the non‐sonicated control. Fragmentations of the mycelia in the sonicated samples were observed in Scanning Electron Microscopy (SEM) micrographs after the US application, supporting the idea of a better release of enzymes to outside the cell.
CONCLUSION
Ultrasound‐assisted fermentation was a promising tool to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66). The combination of US parameters showed to be decisive in obtaining higher enzyme production when compared to the control non‐sonicated experiment. Therefore, this work brings a significant contribution to the field of stimulation of bioprocesses with ultrasound since it shows a strategy to obtain higher enzyme production when compared to traditional fermentation. © 2020 Society of Chemical Industry</description><subject>Beauveria bassiana</subject><subject>Chitinase</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Micrography</subject><subject>Mycelia</subject><subject>Parameters</subject><subject>Photomicrographs</subject><subject>Scanning electron microscopy</subject><subject>submerged fermentation; chitinase; β‐1,3‐glucanase; process intensification; ultrasound stimulation</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OwzAQhS0EEqWw4AaWWCGR1nFcJ17Sil9VYtOuLf-MIVWaFDsBdccROAsH4RCcBKdly2ZGb_TNm9FD6Dwlo5QQOl6ZVo_4JGUHaJASkSeMc3KIBoTyIqGTfHKMTkJYEUJ4QfkArZdV61Voutr-fHyqEMrQgsUO_BrqVrVlU2PXeLzxje3MTjYOf39FOL3KYn2uOqNqFQCr2mLzUrblTuktnoLq3sCXCuveOFKn6MipKsDZXx-i5e3NYnafzJ_uHmbX88RQkbMkMwVwpjUTjCnBtMkLKsBxR5wQAjJQ2goCQKi1hVNcKUg5xIkVucq1yYboYu8b337tILRy1XS-jiclZTynBS_SIlKXe8r4JgQPTm58uVZ-K1Mi-zRln6bs04zseM--lxVs_wfl42wx3W38AlgOfQ0</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Schmaltz, Silvana</creator><creator>Aita, Bruno C</creator><creator>Alves, Eliana A</creator><creator>Fochi, Alex</creator><creator>Bolson, Vinícius F</creator><creator>Navarro‐Díaz, Helmut J</creator><creator>Kuhn, Raquel C</creator><creator>Mazutti, Marcio A</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-0175-044X</orcidid><orcidid>https://orcid.org/0000-0001-8217-5629</orcidid></search><sort><creationdate>202101</creationdate><title>Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana</title><author>Schmaltz, Silvana ; Aita, Bruno C ; Alves, Eliana A ; Fochi, Alex ; Bolson, Vinícius F ; Navarro‐Díaz, Helmut J ; Kuhn, Raquel C ; Mazutti, Marcio A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2974-3c8e64bb4944a94bc7829ef6f0f999e3eabd90ee02dd8fa6aae16e90ed97a7bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Beauveria bassiana</topic><topic>Chitinase</topic><topic>Enzymes</topic><topic>Fermentation</topic><topic>Micrography</topic><topic>Mycelia</topic><topic>Parameters</topic><topic>Photomicrographs</topic><topic>Scanning electron microscopy</topic><topic>submerged fermentation; chitinase; β‐1,3‐glucanase; process intensification; ultrasound stimulation</topic><topic>Ultrasonic imaging</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmaltz, Silvana</creatorcontrib><creatorcontrib>Aita, Bruno C</creatorcontrib><creatorcontrib>Alves, Eliana A</creatorcontrib><creatorcontrib>Fochi, Alex</creatorcontrib><creatorcontrib>Bolson, Vinícius F</creatorcontrib><creatorcontrib>Navarro‐Díaz, Helmut J</creatorcontrib><creatorcontrib>Kuhn, Raquel C</creatorcontrib><creatorcontrib>Mazutti, Marcio A</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmaltz, Silvana</au><au>Aita, Bruno C</au><au>Alves, Eliana A</au><au>Fochi, Alex</au><au>Bolson, Vinícius F</au><au>Navarro‐Díaz, Helmut J</au><au>Kuhn, Raquel C</au><au>Mazutti, Marcio A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><date>2021-01</date><risdate>2021</risdate><volume>96</volume><issue>1</issue><spage>88</spage><epage>98</epage><pages>88-98</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND
Ultrasound (US) treatment was applied to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the parameters evaluated.
RESULTS
The best strategy comprised ultrasonication during 5 min at 24 h of fermentation with a power output of 195 W (fixed frequency of 24 kHz) and a duty cycle of 0.5 s.s−1 (0.5 s ON, 0.5 s OFF). The US treatment resulted in a production increase of 46% for β‐1,3‐glucanase and 42% for chitinase compared to the non‐sonicated control. Fragmentations of the mycelia in the sonicated samples were observed in Scanning Electron Microscopy (SEM) micrographs after the US application, supporting the idea of a better release of enzymes to outside the cell.
CONCLUSION
Ultrasound‐assisted fermentation was a promising tool to increase the production of β‐1,3‐glucanase and chitinase by Beauveria bassiana (IBCB 66). The combination of US parameters showed to be decisive in obtaining higher enzyme production when compared to the control non‐sonicated experiment. Therefore, this work brings a significant contribution to the field of stimulation of bioprocesses with ultrasound since it shows a strategy to obtain higher enzyme production when compared to traditional fermentation. © 2020 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.6514</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0175-044X</orcidid><orcidid>https://orcid.org/0000-0001-8217-5629</orcidid></addata></record> |
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| ispartof | Journal of chemical technology and biotechnology (1986), 2021-01, Vol.96 (1), p.88-98 |
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| language | eng |
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| subjects | Beauveria bassiana Chitinase Enzymes Fermentation Micrography Mycelia Parameters Photomicrographs Scanning electron microscopy submerged fermentation chitinase β‐1,3‐glucanase process intensification ultrasound stimulation Ultrasonic imaging Ultrasound |
| title | Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana |
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