Imaging of Hydroxyl-Radical Generation Using Dynamic Nuclear Polarization-Magnetic Resonance Imaging and a Spin-Trapping Agent
Reactive oxygen species (ROS) play an important role in cell metabolism, but they can cause oxidative damage to biomolecules. Among ROS, the hydroxyl radical (·OH) is one of the most reactive molecules in biological systems because of its high reaction rate constant. Therefore, imaging of ·OH could...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-11, Vol.92 (21), p.14408-14414 |
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| creator | Shoda, Shinichi Hyodo, Fuminori Tachibana, Yoko Kiniwa, Mamoru Naganuma, Tatsuya Eto, Hinako Koyasu, Norikazu Murata, Masaharu Matsuo, Masayuki |
| description | Reactive oxygen species (ROS) play an important role in cell metabolism, but they can cause oxidative damage to biomolecules. Among ROS, the hydroxyl radical (·OH) is one of the most reactive molecules in biological systems because of its high reaction rate constant. Therefore, imaging of ·OH could be useful for evaluation of the redox mechanism and diagnosis of oxidative diseases. In vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) is a noninvasive imaging method to obtain spatiotemporal information about free radicals with MRI anatomical resolution. In this study, we investigated the visualization of hydroxyl radicals generated from the Fenton reaction by combining DNP-MRI with a spin-trapping agent (DMPO: 5,5-dimethyl-1-pyrroline N-oxide) for ·OH. Additionally, we demonstrated the radical-scavenging effect using four thiol-related reagents by DNP-MRI. We demonstrated that DNP enhancement could be induced by the DMPO-OH radical using the DNP-MRI/spin-trapping method and visualized ·OH generation for the first time. Maximum DNP enhancement was observed at an electron paramagnetic resonance irradiation frequency of 474.5 MHz. Furthermore, the radical-scavenging effect was simultaneously evaluated by the decrease in the DNP image value of DMPO-OH. An advantage of our methods is that they simultaneously investigate compound activity and the radical-scavenging effect. |
| doi_str_mv | 10.1021/acs.analchem.0c02331 |
| format | Article |
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Among ROS, the hydroxyl radical (·OH) is one of the most reactive molecules in biological systems because of its high reaction rate constant. Therefore, imaging of ·OH could be useful for evaluation of the redox mechanism and diagnosis of oxidative diseases. In vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) is a noninvasive imaging method to obtain spatiotemporal information about free radicals with MRI anatomical resolution. In this study, we investigated the visualization of hydroxyl radicals generated from the Fenton reaction by combining DNP-MRI with a spin-trapping agent (DMPO: 5,5-dimethyl-1-pyrroline N-oxide) for ·OH. Additionally, we demonstrated the radical-scavenging effect using four thiol-related reagents by DNP-MRI. We demonstrated that DNP enhancement could be induced by the DMPO-OH radical using the DNP-MRI/spin-trapping method and visualized ·OH generation for the first time. Maximum DNP enhancement was observed at an electron paramagnetic resonance irradiation frequency of 474.5 MHz. Furthermore, the radical-scavenging effect was simultaneously evaluated by the decrease in the DNP image value of DMPO-OH. An advantage of our methods is that they simultaneously investigate compound activity and the radical-scavenging effect.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c02331</identifier><identifier>PMID: 33064938</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Biomolecules ; Chemistry ; Cyclic N-Oxides - chemistry ; Electron paramagnetic resonance ; Electron spin resonance ; Free radicals ; Hydrogen Peroxide - chemistry ; Hydroxyl Radical - chemistry ; Hydroxyl Radical - metabolism ; Hydroxyl radicals ; In vivo methods and tests ; Iron - chemistry ; Irradiation ; Magnetic Resonance Imaging ; Medical imaging ; Metabolism ; Oxidation ; Polarization ; Radiation ; Reactive oxygen species ; Reagents ; Resonance ; Scavenging ; Spin Trapping ; Time Factors ; Trapping</subject><ispartof>Analytical chemistry (Washington), 2020-11, Vol.92 (21), p.14408-14414</ispartof><rights>2020 American Chemical Society</rights><rights>Copyright American Chemical Society Nov 3, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-c48e832ff104827e6308e34d1c24b9c1b22d2c7ce8cec7a995bcdf010f4c8df63</citedby><cites>FETCH-LOGICAL-a442t-c48e832ff104827e6308e34d1c24b9c1b22d2c7ce8cec7a995bcdf010f4c8df63</cites><orcidid>0000-0002-9266-9937</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c02331$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c02331$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33064938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shoda, Shinichi</creatorcontrib><creatorcontrib>Hyodo, Fuminori</creatorcontrib><creatorcontrib>Tachibana, Yoko</creatorcontrib><creatorcontrib>Kiniwa, Mamoru</creatorcontrib><creatorcontrib>Naganuma, Tatsuya</creatorcontrib><creatorcontrib>Eto, Hinako</creatorcontrib><creatorcontrib>Koyasu, Norikazu</creatorcontrib><creatorcontrib>Murata, Masaharu</creatorcontrib><creatorcontrib>Matsuo, Masayuki</creatorcontrib><title>Imaging of Hydroxyl-Radical Generation Using Dynamic Nuclear Polarization-Magnetic Resonance Imaging and a Spin-Trapping Agent</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Reactive oxygen species (ROS) play an important role in cell metabolism, but they can cause oxidative damage to biomolecules. Among ROS, the hydroxyl radical (·OH) is one of the most reactive molecules in biological systems because of its high reaction rate constant. Therefore, imaging of ·OH could be useful for evaluation of the redox mechanism and diagnosis of oxidative diseases. In vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) is a noninvasive imaging method to obtain spatiotemporal information about free radicals with MRI anatomical resolution. In this study, we investigated the visualization of hydroxyl radicals generated from the Fenton reaction by combining DNP-MRI with a spin-trapping agent (DMPO: 5,5-dimethyl-1-pyrroline N-oxide) for ·OH. Additionally, we demonstrated the radical-scavenging effect using four thiol-related reagents by DNP-MRI. We demonstrated that DNP enhancement could be induced by the DMPO-OH radical using the DNP-MRI/spin-trapping method and visualized ·OH generation for the first time. Maximum DNP enhancement was observed at an electron paramagnetic resonance irradiation frequency of 474.5 MHz. Furthermore, the radical-scavenging effect was simultaneously evaluated by the decrease in the DNP image value of DMPO-OH. An advantage of our methods is that they simultaneously investigate compound activity and the radical-scavenging effect.</description><subject>Analytical chemistry</subject><subject>Biomolecules</subject><subject>Chemistry</subject><subject>Cyclic N-Oxides - chemistry</subject><subject>Electron paramagnetic resonance</subject><subject>Electron spin resonance</subject><subject>Free radicals</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Hydroxyl Radical - chemistry</subject><subject>Hydroxyl Radical - metabolism</subject><subject>Hydroxyl radicals</subject><subject>In vivo methods and tests</subject><subject>Iron - chemistry</subject><subject>Irradiation</subject><subject>Magnetic Resonance Imaging</subject><subject>Medical imaging</subject><subject>Metabolism</subject><subject>Oxidation</subject><subject>Polarization</subject><subject>Radiation</subject><subject>Reactive oxygen species</subject><subject>Reagents</subject><subject>Resonance</subject><subject>Scavenging</subject><subject>Spin Trapping</subject><subject>Time Factors</subject><subject>Trapping</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P20AQhldVEQTKP6iqlXp2OvsRe3OMKA2RKCAKZ2syHqeO7LW760gNB347TpNw7Gmkmed9R3qE-KxgrECrb0hxjB5r-s3NGAi0MeqDGKmJhiR1Tn8UIwAwic4AzsR5jGsApUClp-LMGEjt1LiReF00uKr8SralvNkWof27rZNHLCrCWs7Zc8C-ar18jjvo-9ZjU5G821DNGORDW2OoXv4hyU9cee6H6yPH1qMnlsdy9IVE-aurfPIUsOt2u9mKff9JnJRYR748zAvx_OP66eomub2fL65mtwlaq_uErGNndFkqsE5nnBpwbGyhSNvllNRS60JTRuyIKcPpdLKkogQFpSVXlKm5EF_3vV1o_2w49vm63YTBXsy1TVOTZpOJHSi7pyi0MQYu8y5UDYZtriDfSc8H6flRen6QPsS-HMo3y4aL99DR8gDAHtjF3x__t_MN34CSdQ</recordid><startdate>20201103</startdate><enddate>20201103</enddate><creator>Shoda, Shinichi</creator><creator>Hyodo, Fuminori</creator><creator>Tachibana, Yoko</creator><creator>Kiniwa, Mamoru</creator><creator>Naganuma, Tatsuya</creator><creator>Eto, Hinako</creator><creator>Koyasu, Norikazu</creator><creator>Murata, Masaharu</creator><creator>Matsuo, Masayuki</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-9266-9937</orcidid></search><sort><creationdate>20201103</creationdate><title>Imaging of Hydroxyl-Radical Generation Using Dynamic Nuclear Polarization-Magnetic Resonance Imaging and a Spin-Trapping Agent</title><author>Shoda, Shinichi ; Hyodo, Fuminori ; Tachibana, Yoko ; Kiniwa, Mamoru ; Naganuma, Tatsuya ; Eto, Hinako ; Koyasu, Norikazu ; Murata, Masaharu ; Matsuo, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-c48e832ff104827e6308e34d1c24b9c1b22d2c7ce8cec7a995bcdf010f4c8df63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analytical chemistry</topic><topic>Biomolecules</topic><topic>Chemistry</topic><topic>Cyclic N-Oxides - chemistry</topic><topic>Electron paramagnetic resonance</topic><topic>Electron spin resonance</topic><topic>Free radicals</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Hydroxyl Radical - chemistry</topic><topic>Hydroxyl Radical - metabolism</topic><topic>Hydroxyl radicals</topic><topic>In vivo methods and tests</topic><topic>Iron - chemistry</topic><topic>Irradiation</topic><topic>Magnetic Resonance Imaging</topic><topic>Medical imaging</topic><topic>Metabolism</topic><topic>Oxidation</topic><topic>Polarization</topic><topic>Radiation</topic><topic>Reactive oxygen species</topic><topic>Reagents</topic><topic>Resonance</topic><topic>Scavenging</topic><topic>Spin Trapping</topic><topic>Time Factors</topic><topic>Trapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shoda, Shinichi</creatorcontrib><creatorcontrib>Hyodo, Fuminori</creatorcontrib><creatorcontrib>Tachibana, Yoko</creatorcontrib><creatorcontrib>Kiniwa, Mamoru</creatorcontrib><creatorcontrib>Naganuma, Tatsuya</creatorcontrib><creatorcontrib>Eto, Hinako</creatorcontrib><creatorcontrib>Koyasu, Norikazu</creatorcontrib><creatorcontrib>Murata, Masaharu</creatorcontrib><creatorcontrib>Matsuo, Masayuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shoda, Shinichi</au><au>Hyodo, Fuminori</au><au>Tachibana, Yoko</au><au>Kiniwa, Mamoru</au><au>Naganuma, Tatsuya</au><au>Eto, Hinako</au><au>Koyasu, Norikazu</au><au>Murata, Masaharu</au><au>Matsuo, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging of Hydroxyl-Radical Generation Using Dynamic Nuclear Polarization-Magnetic Resonance Imaging and a Spin-Trapping Agent</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2020-11-03</date><risdate>2020</risdate><volume>92</volume><issue>21</issue><spage>14408</spage><epage>14414</epage><pages>14408-14414</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Reactive oxygen species (ROS) play an important role in cell metabolism, but they can cause oxidative damage to biomolecules. Among ROS, the hydroxyl radical (·OH) is one of the most reactive molecules in biological systems because of its high reaction rate constant. Therefore, imaging of ·OH could be useful for evaluation of the redox mechanism and diagnosis of oxidative diseases. In vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) is a noninvasive imaging method to obtain spatiotemporal information about free radicals with MRI anatomical resolution. In this study, we investigated the visualization of hydroxyl radicals generated from the Fenton reaction by combining DNP-MRI with a spin-trapping agent (DMPO: 5,5-dimethyl-1-pyrroline N-oxide) for ·OH. Additionally, we demonstrated the radical-scavenging effect using four thiol-related reagents by DNP-MRI. We demonstrated that DNP enhancement could be induced by the DMPO-OH radical using the DNP-MRI/spin-trapping method and visualized ·OH generation for the first time. Maximum DNP enhancement was observed at an electron paramagnetic resonance irradiation frequency of 474.5 MHz. Furthermore, the radical-scavenging effect was simultaneously evaluated by the decrease in the DNP image value of DMPO-OH. An advantage of our methods is that they simultaneously investigate compound activity and the radical-scavenging effect.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33064938</pmid><doi>10.1021/acs.analchem.0c02331</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9266-9937</orcidid></addata></record> |
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| subjects | Analytical chemistry Biomolecules Chemistry Cyclic N-Oxides - chemistry Electron paramagnetic resonance Electron spin resonance Free radicals Hydrogen Peroxide - chemistry Hydroxyl Radical - chemistry Hydroxyl Radical - metabolism Hydroxyl radicals In vivo methods and tests Iron - chemistry Irradiation Magnetic Resonance Imaging Medical imaging Metabolism Oxidation Polarization Radiation Reactive oxygen species Reagents Resonance Scavenging Spin Trapping Time Factors Trapping |
| title | Imaging of Hydroxyl-Radical Generation Using Dynamic Nuclear Polarization-Magnetic Resonance Imaging and a Spin-Trapping Agent |
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