Analysis of Cell Spheroid Morphological Characteristics Using the Spheroid Morphology Evaluation System
Our research group established a technology for forming three-dimensional cell constructs to regenerate osteochondro cells without scaffolding. The established technology employed spheroids to form cell constructs. We also developed a method for arranging spheroids in arbitrary positions to form cel...
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| Veröffentlicht in: | Journal of robotics and mechatronics 2018-10, Vol.30 (5), p.819-826 |
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| container_title | Journal of robotics and mechatronics |
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| creator | Shimoto, Takeshi Zhang, Xiu-Ying Akieda, Shizuka Ishikawa, Atsushi Higaki, Hidehiko Nakayama, Koichi |
| description | Our research group established a technology for forming three-dimensional cell constructs to regenerate osteochondro cells without scaffolding. The established technology employed spheroids to form cell constructs. We also developed a method for arranging spheroids in arbitrary positions to form cell constructs in complex shapes. However, we could only form cell constructs as expected when the formed spheroids were the appropriate sizes. This study, therefore, aimed to chronologically analyze the spheroid morphological characteristics of rabbit mechanical stem cells using the developed spheroid morphological evaluation system. We set the numbers of cells/wells as 2 × 10
4
, 3 × 10
4
, 4 × 10
4
, 5 × 10
4
, 6 × 10
4
, and 7 × 10
4
and the passage number as 7. Further, we observed the cultured spheroids every 24 hours after seeding for five days. The analysis enabled us to specify an optimal range for the numbers of cells required to form spheroids with high degrees of circularity. We could also control the formed spheroid sizes by adjusting the cell count and culturing time. |
| doi_str_mv | 10.20965/jrm.2018.p0819 |
| format | Article |
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4
, 3 × 10
4
, 4 × 10
4
, 5 × 10
4
, 6 × 10
4
, and 7 × 10
4
and the passage number as 7. Further, we observed the cultured spheroids every 24 hours after seeding for five days. The analysis enabled us to specify an optimal range for the numbers of cells required to form spheroids with high degrees of circularity. We could also control the formed spheroid sizes by adjusting the cell count and culturing time.</description><identifier>ISSN: 0915-3942</identifier><identifier>EISSN: 1883-8049</identifier><identifier>DOI: 10.20965/jrm.2018.p0819</identifier><language>eng</language><publisher>Tokyo: Fuji Technology Press Co. Ltd</publisher><subject>Biomedical materials ; Morphology ; Scaffolding ; Spheroids ; Stem cells</subject><ispartof>Journal of robotics and mechatronics, 2018-10, Vol.30 (5), p.819-826</ispartof><rights>Copyright © 2018 Fuji Technology Press Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-1f5d0c714a020f4ce88c728daa98879dbf5aa28599c0490878235555d5eb78423</citedby><cites>FETCH-LOGICAL-c460t-1f5d0c714a020f4ce88c728daa98879dbf5aa28599c0490878235555d5eb78423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,865,27929,27930</link.rule.ids></links><search><creatorcontrib>Shimoto, Takeshi</creatorcontrib><creatorcontrib>Zhang, Xiu-Ying</creatorcontrib><creatorcontrib>Akieda, Shizuka</creatorcontrib><creatorcontrib>Ishikawa, Atsushi</creatorcontrib><creatorcontrib>Higaki, Hidehiko</creatorcontrib><creatorcontrib>Nakayama, Koichi</creatorcontrib><creatorcontrib>Saga University 5-1-1 Nabeshima, Saga 849-8501, Japan</creatorcontrib><creatorcontrib>Fukuoka Institute of Technology 3-30-1 Wajiro-higashi, Higashi-ku, Fukuoka 811-0295, Japan</creatorcontrib><creatorcontrib>Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan</creatorcontrib><creatorcontrib>Kyushu Sangyo University 2-3-1 Matsukadai, Higashi-ku, Fukuoka 813-8503, Japan</creatorcontrib><creatorcontrib>Cyfuse Biomedical K.K. 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan</creatorcontrib><title>Analysis of Cell Spheroid Morphological Characteristics Using the Spheroid Morphology Evaluation System</title><title>Journal of robotics and mechatronics</title><description>Our research group established a technology for forming three-dimensional cell constructs to regenerate osteochondro cells without scaffolding. The established technology employed spheroids to form cell constructs. We also developed a method for arranging spheroids in arbitrary positions to form cell constructs in complex shapes. However, we could only form cell constructs as expected when the formed spheroids were the appropriate sizes. This study, therefore, aimed to chronologically analyze the spheroid morphological characteristics of rabbit mechanical stem cells using the developed spheroid morphological evaluation system. We set the numbers of cells/wells as 2 × 10
4
, 3 × 10
4
, 4 × 10
4
, 5 × 10
4
, 6 × 10
4
, and 7 × 10
4
and the passage number as 7. Further, we observed the cultured spheroids every 24 hours after seeding for five days. The analysis enabled us to specify an optimal range for the numbers of cells required to form spheroids with high degrees of circularity. We could also control the formed spheroid sizes by adjusting the cell count and culturing time.</description><subject>Biomedical materials</subject><subject>Morphology</subject><subject>Scaffolding</subject><subject>Spheroids</subject><subject>Stem cells</subject><issn>0915-3942</issn><issn>1883-8049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkL1vwjAUxK2qlYooc1dLnQP-TJ5HFNEPiaoDZbaM4xCjgFM7VMp_3xQ69pZ3w72T7ofQIyVzRlQuF4d4HB2FeUeAqhs0oQA8AyLULZoQRWXGlWD3aJbSgYySolC8mKD98mTaIfmEQ41L17Z40zUuBl_h9xC7JrRh761pcdmYaGzvok-9twlvkz_tcd-4fx4GvPo27dn0PpzwZki9Oz6gu9q0yc3-7hRtn1ef5Wu2_nh5K5frzIqc9BmtZUVsQYUhjNTCOgBbMKiMUQCFqna1NIaBVMqO0wgUwLgcVUm3K0AwPkVP194uhq-zS70-hHMcNybNRC6B8jwXY2pxTdkYUoqu1l30RxMHTYm-ANUjUP0LVF-A8h-O42o6</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Shimoto, Takeshi</creator><creator>Zhang, Xiu-Ying</creator><creator>Akieda, Shizuka</creator><creator>Ishikawa, Atsushi</creator><creator>Higaki, Hidehiko</creator><creator>Nakayama, Koichi</creator><general>Fuji Technology Press Co. 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The established technology employed spheroids to form cell constructs. We also developed a method for arranging spheroids in arbitrary positions to form cell constructs in complex shapes. However, we could only form cell constructs as expected when the formed spheroids were the appropriate sizes. This study, therefore, aimed to chronologically analyze the spheroid morphological characteristics of rabbit mechanical stem cells using the developed spheroid morphological evaluation system. We set the numbers of cells/wells as 2 × 10
4
, 3 × 10
4
, 4 × 10
4
, 5 × 10
4
, 6 × 10
4
, and 7 × 10
4
and the passage number as 7. Further, we observed the cultured spheroids every 24 hours after seeding for five days. The analysis enabled us to specify an optimal range for the numbers of cells required to form spheroids with high degrees of circularity. We could also control the formed spheroid sizes by adjusting the cell count and culturing time.</abstract><cop>Tokyo</cop><pub>Fuji Technology Press Co. Ltd</pub><doi>10.20965/jrm.2018.p0819</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan |
| subjects | Biomedical materials Morphology Scaffolding Spheroids Stem cells |
| title | Analysis of Cell Spheroid Morphological Characteristics Using the Spheroid Morphology Evaluation System |
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