Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgr...

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Veröffentlicht in:Applied sciences 2020-11, Vol.10 (22), p.8289
Hauptverfasser: Catizone, Angela, Morabito, Caterina, Cammarota, Marcella, Schiraldi, Chiara, Scheri, Katia Corano, Ferranti, Francesca, Mariggio, Maria A, Ricci, Giulia
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container_issue 22
container_start_page 8289
container_title Applied sciences
container_volume 10
creator Catizone, Angela
Morabito, Caterina
Cammarota, Marcella
Schiraldi, Chiara
Scheri, Katia Corano
Ferranti, Francesca
Mariggio, Maria A
Ricci, Giulia
description The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.
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Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. 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subjects Antioxidants
Autophagy
Biotechnology
Cadherin
Cell adhesion
Cloning
Clustering
Confocal microscopy
Disorientation
Ethanol
Exposure
Germ cells
Glycerol
Gravity
Immunofluorescence
Membranes
Metabolism
Microgravity
Phagocytosis
Physiological aspects
Physiology
Proteins
Scanning electron microscopy
Seminoma
Transmission electron microscopy
β-Catenin
title Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells
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