Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells
The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgr...
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creator | Catizone, Angela Morabito, Caterina Cammarota, Marcella Schiraldi, Chiara Scheri, Katia Corano Ferranti, Francesca Mariggio, Maria A Ricci, Giulia |
description | The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules. |
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In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.</description><identifier>ISSN: 2076-3417</identifier><identifier>EISSN: 2076-3417</identifier><identifier>DOI: 10.3390/app10228289</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Antioxidants ; Autophagy ; Biotechnology ; Cadherin ; Cell adhesion ; Cloning ; Clustering ; Confocal microscopy ; Disorientation ; Ethanol ; Exposure ; Germ cells ; Glycerol ; Gravity ; Immunofluorescence ; Membranes ; Metabolism ; Microgravity ; Phagocytosis ; Physiological aspects ; Physiology ; Proteins ; Scanning electron microscopy ; Seminoma ; Transmission electron microscopy ; β-Catenin</subject><ispartof>Applied sciences, 2020-11, Vol.10 (22), p.8289</ispartof><rights>COPYRIGHT 2020 MDPI AG</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-1e53227496e9377120bd001d802bffbd736389022997d1d9555b6ca0f328b0bd3</citedby><cites>FETCH-LOGICAL-c365t-1e53227496e9377120bd001d802bffbd736389022997d1d9555b6ca0f328b0bd3</cites><orcidid>0000-0002-2199-170X ; 0000-0002-1066-843X ; 0000-0001-5306-7143 ; 0000-0002-7836-9495 ; 0000-0002-5144-2584</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Catizone, Angela</creatorcontrib><creatorcontrib>Morabito, Caterina</creatorcontrib><creatorcontrib>Cammarota, Marcella</creatorcontrib><creatorcontrib>Schiraldi, Chiara</creatorcontrib><creatorcontrib>Scheri, Katia Corano</creatorcontrib><creatorcontrib>Ferranti, Francesca</creatorcontrib><creatorcontrib>Mariggio, Maria A</creatorcontrib><creatorcontrib>Ricci, Giulia</creatorcontrib><title>Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells</title><title>Applied sciences</title><description>The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. 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Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.</description><subject>Antioxidants</subject><subject>Autophagy</subject><subject>Biotechnology</subject><subject>Cadherin</subject><subject>Cell adhesion</subject><subject>Cloning</subject><subject>Clustering</subject><subject>Confocal microscopy</subject><subject>Disorientation</subject><subject>Ethanol</subject><subject>Exposure</subject><subject>Germ cells</subject><subject>Glycerol</subject><subject>Gravity</subject><subject>Immunofluorescence</subject><subject>Membranes</subject><subject>Metabolism</subject><subject>Microgravity</subject><subject>Phagocytosis</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>Seminoma</subject><subject>Transmission electron microscopy</subject><subject>β-Catenin</subject><issn>2076-3417</issn><issn>2076-3417</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkU1LAzEQhoMoWGpP_oEFj7I1H_uRHMviR6XiwXoO2U22pOwma5KV9t-bWsEKJoeZyTzvwJsB4BrBOSEM3olhQBBjiik7AxMMyyIlGSrPT_JLMPN-C-NhiFAEJ2D3ohtnN0586rBPl0aOjZJJpbouDTY9xOR5NE3Q1oguqawJogk-WTgVi9EE5WIdFfU-WZhI7bQUJsReP8S29Ik2yboSfYqTN9VrY3vxPd1fgYtWdF7NfuIUvD_cr6undPX6uKwWq7QhRR5SpHKCcZmxQjFSlgjDWkKIJIW4bttalqQglEXbjJUSSZbneV00ArYE0zqyZApujnMHZz9G5QPf2tFFM57jrMgYYZiiX2ojOsW1aW2IxnrtG74osiIvM4phpOb_UPHKaK2xRrU6vv8R3B4F8Y-9d6rlg9O9cHuOID8sjZ8sjXwBu82Hig</recordid><startdate>20201115</startdate><enddate>20201115</enddate><creator>Catizone, Angela</creator><creator>Morabito, Caterina</creator><creator>Cammarota, Marcella</creator><creator>Schiraldi, Chiara</creator><creator>Scheri, Katia Corano</creator><creator>Ferranti, Francesca</creator><creator>Mariggio, Maria A</creator><creator>Ricci, Giulia</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-2199-170X</orcidid><orcidid>https://orcid.org/0000-0002-1066-843X</orcidid><orcidid>https://orcid.org/0000-0001-5306-7143</orcidid><orcidid>https://orcid.org/0000-0002-7836-9495</orcidid><orcidid>https://orcid.org/0000-0002-5144-2584</orcidid></search><sort><creationdate>20201115</creationdate><title>Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells</title><author>Catizone, Angela ; Morabito, Caterina ; Cammarota, Marcella ; Schiraldi, Chiara ; Scheri, Katia Corano ; Ferranti, Francesca ; Mariggio, Maria A ; Ricci, Giulia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-1e53227496e9377120bd001d802bffbd736389022997d1d9555b6ca0f328b0bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidants</topic><topic>Autophagy</topic><topic>Biotechnology</topic><topic>Cadherin</topic><topic>Cell adhesion</topic><topic>Cloning</topic><topic>Clustering</topic><topic>Confocal microscopy</topic><topic>Disorientation</topic><topic>Ethanol</topic><topic>Exposure</topic><topic>Germ cells</topic><topic>Glycerol</topic><topic>Gravity</topic><topic>Immunofluorescence</topic><topic>Membranes</topic><topic>Metabolism</topic><topic>Microgravity</topic><topic>Phagocytosis</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>Seminoma</topic><topic>Transmission electron microscopy</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Catizone, Angela</creatorcontrib><creatorcontrib>Morabito, Caterina</creatorcontrib><creatorcontrib>Cammarota, Marcella</creatorcontrib><creatorcontrib>Schiraldi, Chiara</creatorcontrib><creatorcontrib>Scheri, Katia Corano</creatorcontrib><creatorcontrib>Ferranti, Francesca</creatorcontrib><creatorcontrib>Mariggio, Maria A</creatorcontrib><creatorcontrib>Ricci, Giulia</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Applied sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Catizone, Angela</au><au>Morabito, Caterina</au><au>Cammarota, Marcella</au><au>Schiraldi, Chiara</au><au>Scheri, Katia Corano</au><au>Ferranti, Francesca</au><au>Mariggio, Maria A</au><au>Ricci, Giulia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells</atitle><jtitle>Applied sciences</jtitle><date>2020-11-15</date><risdate>2020</risdate><volume>10</volume><issue>22</issue><spage>8289</spage><pages>8289-</pages><issn>2076-3417</issn><eissn>2076-3417</eissn><abstract>The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. 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subjects | Antioxidants Autophagy Biotechnology Cadherin Cell adhesion Cloning Clustering Confocal microscopy Disorientation Ethanol Exposure Germ cells Glycerol Gravity Immunofluorescence Membranes Metabolism Microgravity Phagocytosis Physiological aspects Physiology Proteins Scanning electron microscopy Seminoma Transmission electron microscopy β-Catenin |
title | Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells |
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