Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium
ABSTRACT Toxic effect of sub-lethal concentration(LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain(Lab-S) and deltamethrin resistant population(GUW) of stored grain pest, Tro...
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| Veröffentlicht in: | Pakistan journal of zoology 2020-12, Vol.52 (6), p.2161 |
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| description | ABSTRACT Toxic effect of sub-lethal concentration(LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain(Lab-S) and deltamethrin resistant population(GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae of Lab-S and GUW populations when compared with their respective controls. Acetylcholinesterase(AchE) activity was significantly decreased(28.94 and 42.42%) in the 4th instar larvae of Lab-S population after treatments with bifenthrin and bifenthrin: chlorpyrifos combination 3:1, respectively. After exposure to chlorpyrifos and bifenthrin: chlorpyrifos combination 1:3 the AchE activity was increased 45.24 and 64.28%, respectively. This increase was much more prominent in the 6th instar larvae viz., 93.95, 45.57, 182.52 and 132.61% following treatments with bifenthrin, chlorpyrifos, 3:1 and 1:3 combinations of two insecticides, respectively. In the resistant population this increase was 35.71, 48.10 and 75.61% in the 4th instar, respectively and 9.47, 28.17 and 60.29% in the 6th instar after chlorpyrifos, 3:1 combination and 1:3 combination treatments, respectively. This activity was decreased 63.99% in the 6th instar but increased in the 4th instar 13.36% after bifenthrin exposure. Arylesterase activity was declined after exposure to bifenthrin, chlorpyrifos and 3:1 combination in both the 4th instar(89.47, 64.33 and 36.65%, respectively) and 6th instar(84.49, 54.01, 4.28%, respectively) of Lab-S as well as in the 4th instar(77.73, 55.81 and 35.96%, respectively) of resistant population. This activity was upregulated(84.92, 141.86 and 192.88%, respectively) in the 6th instar of resistant population, following exposures to bifenthrin, chlorpyrifos and 3:1 combination. Whereas, this activity was increased in the 4th instar(53.22%) and 6th instar(124.60%) of Lab-S population as well as in the 4th instar(35.19%) and 6th instar(234.75%) of resistant population after treatment with 1:3 combination, respectively. The findings of current investigation suggests that insects upregulate the synthesis of esterases in general cholinesterase and carboxylesterase in response to exposure to insecticide to combat with the stressful condition |
| doi_str_mv | 10.17582/journal.pjz/20200501083425 |
| format | Article |
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After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae of Lab-S and GUW populations when compared with their respective controls. Acetylcholinesterase(AchE) activity was significantly decreased(28.94 and 42.42%) in the 4th instar larvae of Lab-S population after treatments with bifenthrin and bifenthrin: chlorpyrifos combination 3:1, respectively. After exposure to chlorpyrifos and bifenthrin: chlorpyrifos combination 1:3 the AchE activity was increased 45.24 and 64.28%, respectively. This increase was much more prominent in the 6th instar larvae viz., 93.95, 45.57, 182.52 and 132.61% following treatments with bifenthrin, chlorpyrifos, 3:1 and 1:3 combinations of two insecticides, respectively. In the resistant population this increase was 35.71, 48.10 and 75.61% in the 4th instar, respectively and 9.47, 28.17 and 60.29% in the 6th instar after chlorpyrifos, 3:1 combination and 1:3 combination treatments, respectively. This activity was decreased 63.99% in the 6th instar but increased in the 4th instar 13.36% after bifenthrin exposure. Arylesterase activity was declined after exposure to bifenthrin, chlorpyrifos and 3:1 combination in both the 4th instar(89.47, 64.33 and 36.65%, respectively) and 6th instar(84.49, 54.01, 4.28%, respectively) of Lab-S as well as in the 4th instar(77.73, 55.81 and 35.96%, respectively) of resistant population. This activity was upregulated(84.92, 141.86 and 192.88%, respectively) in the 6th instar of resistant population, following exposures to bifenthrin, chlorpyrifos and 3:1 combination. Whereas, this activity was increased in the 4th instar(53.22%) and 6th instar(124.60%) of Lab-S population as well as in the 4th instar(35.19%) and 6th instar(234.75%) of resistant population after treatment with 1:3 combination, respectively. The findings of current investigation suggests that insects upregulate the synthesis of esterases in general cholinesterase and carboxylesterase in response to exposure to insecticide to combat with the stressful conditions of exposure to toxic chemicals. The activity of the arylesterase, on the contrary is inhibited to variable extent after exposure to the insecticides.</description><identifier>ISSN: 0030-9923</identifier><identifier>DOI: 10.17582/journal.pjz/20200501083425</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>Acetylcholinesterase ; Arylesterase ; Beetles ; Carboxylesterase ; Chemical activity ; Chlorpyrifos ; Cholinesterase ; Deltamethrin ; Endangered & extinct species ; Esterases ; Exposure ; Grain storage ; Health aspects ; Insecticide resistance ; Insecticides ; Insects ; Larvae ; Mortality ; Pest resistance ; Pesticides ; Pests ; Population ; Pyrethroids ; Trogoderma granarium ; Variance analysis</subject><ispartof>Pakistan journal of zoology, 2020-12, Vol.52 (6), p.2161</ispartof><rights>COPYRIGHT 2020 Knowledge Bylanes</rights><rights>(c)2020 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-840529568c36797733dbff69ab7ce00529ab412b1c2de367a68b77b320d6e2cc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Feroz, Anum</creatorcontrib><creatorcontrib>Shakoori, Abdul Rauf</creatorcontrib><creatorcontrib>Shakoori, Farah Rauf</creatorcontrib><title>Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium</title><title>Pakistan journal of zoology</title><description>ABSTRACT Toxic effect of sub-lethal concentration(LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain(Lab-S) and deltamethrin resistant population(GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae of Lab-S and GUW populations when compared with their respective controls. Acetylcholinesterase(AchE) activity was significantly decreased(28.94 and 42.42%) in the 4th instar larvae of Lab-S population after treatments with bifenthrin and bifenthrin: chlorpyrifos combination 3:1, respectively. After exposure to chlorpyrifos and bifenthrin: chlorpyrifos combination 1:3 the AchE activity was increased 45.24 and 64.28%, respectively. This increase was much more prominent in the 6th instar larvae viz., 93.95, 45.57, 182.52 and 132.61% following treatments with bifenthrin, chlorpyrifos, 3:1 and 1:3 combinations of two insecticides, respectively. In the resistant population this increase was 35.71, 48.10 and 75.61% in the 4th instar, respectively and 9.47, 28.17 and 60.29% in the 6th instar after chlorpyrifos, 3:1 combination and 1:3 combination treatments, respectively. This activity was decreased 63.99% in the 6th instar but increased in the 4th instar 13.36% after bifenthrin exposure. Arylesterase activity was declined after exposure to bifenthrin, chlorpyrifos and 3:1 combination in both the 4th instar(89.47, 64.33 and 36.65%, respectively) and 6th instar(84.49, 54.01, 4.28%, respectively) of Lab-S as well as in the 4th instar(77.73, 55.81 and 35.96%, respectively) of resistant population. This activity was upregulated(84.92, 141.86 and 192.88%, respectively) in the 6th instar of resistant population, following exposures to bifenthrin, chlorpyrifos and 3:1 combination. Whereas, this activity was increased in the 4th instar(53.22%) and 6th instar(124.60%) of Lab-S population as well as in the 4th instar(35.19%) and 6th instar(234.75%) of resistant population after treatment with 1:3 combination, respectively. The findings of current investigation suggests that insects upregulate the synthesis of esterases in general cholinesterase and carboxylesterase in response to exposure to insecticide to combat with the stressful conditions of exposure to toxic chemicals. The activity of the arylesterase, on the contrary is inhibited to variable extent after exposure to the insecticides.</description><subject>Acetylcholinesterase</subject><subject>Arylesterase</subject><subject>Beetles</subject><subject>Carboxylesterase</subject><subject>Chemical activity</subject><subject>Chlorpyrifos</subject><subject>Cholinesterase</subject><subject>Deltamethrin</subject><subject>Endangered & extinct species</subject><subject>Esterases</subject><subject>Exposure</subject><subject>Grain storage</subject><subject>Health aspects</subject><subject>Insecticide resistance</subject><subject>Insecticides</subject><subject>Insects</subject><subject>Larvae</subject><subject>Mortality</subject><subject>Pest resistance</subject><subject>Pesticides</subject><subject>Pests</subject><subject>Population</subject><subject>Pyrethroids</subject><subject>Trogoderma granarium</subject><subject>Variance analysis</subject><issn>0030-9923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkc9KAzEQxvegYKm-Q8CrbWeT3WQXT7XWPyAotJ5DNpu0KbtJTdKDvoiva6oVKZg5BGZ-38cwX5Zd5jDOWVnhycbtvBXdeLv5mGDAACXkUJEClyfZAIDAqK4xOcsuQthAegUtMa4G2edcayUjchotdk2n4lp06NYFFfatG6OVjWtvLBK2RbN15_z23RvtApq2vbEmROVVi6ads-qbSejM9Y2xIhpnk4tF8z0kDpaL6PaCey8S-aJCvEJL71auVb4XaOWFFd7s-vPsVIsuqIvDP8xe7-bL2cPo6fn-cTZ9GskiJ3FUFVDiuqSVJJTVjBHSNlrTWjRMKtjPRFPkuMklblVCBK0axhqCoaUKS0mG2eWP79a7t11ahx8uGTguKCYM8oL-USvRKW6sdtEL2Zsg-ZQWmABQhhM1_odK1areyHQgbVL_SHD9I5DeheCV5ltveuHfeQ78O9ffbXjKlR_nSr4Afmab7g</recordid><startdate>20201231</startdate><enddate>20201231</enddate><creator>Feroz, Anum</creator><creator>Shakoori, Abdul Rauf</creator><creator>Shakoori, Farah Rauf</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SS</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20201231</creationdate><title>Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium</title><author>Feroz, Anum ; Shakoori, Abdul Rauf ; Shakoori, Farah Rauf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-840529568c36797733dbff69ab7ce00529ab412b1c2de367a68b77b320d6e2cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetylcholinesterase</topic><topic>Arylesterase</topic><topic>Beetles</topic><topic>Carboxylesterase</topic><topic>Chemical activity</topic><topic>Chlorpyrifos</topic><topic>Cholinesterase</topic><topic>Deltamethrin</topic><topic>Endangered & extinct species</topic><topic>Esterases</topic><topic>Exposure</topic><topic>Grain storage</topic><topic>Health aspects</topic><topic>Insecticide resistance</topic><topic>Insecticides</topic><topic>Insects</topic><topic>Larvae</topic><topic>Mortality</topic><topic>Pest resistance</topic><topic>Pesticides</topic><topic>Pests</topic><topic>Population</topic><topic>Pyrethroids</topic><topic>Trogoderma granarium</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feroz, Anum</creatorcontrib><creatorcontrib>Shakoori, Abdul Rauf</creatorcontrib><creatorcontrib>Shakoori, Farah Rauf</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Pakistan journal of zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feroz, Anum</au><au>Shakoori, Abdul Rauf</au><au>Shakoori, Farah Rauf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium</atitle><jtitle>Pakistan journal of zoology</jtitle><date>2020-12-31</date><risdate>2020</risdate><volume>52</volume><issue>6</issue><spage>2161</spage><pages>2161-</pages><issn>0030-9923</issn><abstract>ABSTRACT Toxic effect of sub-lethal concentration(LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain(Lab-S) and deltamethrin resistant population(GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae of Lab-S and GUW populations when compared with their respective controls. Acetylcholinesterase(AchE) activity was significantly decreased(28.94 and 42.42%) in the 4th instar larvae of Lab-S population after treatments with bifenthrin and bifenthrin: chlorpyrifos combination 3:1, respectively. After exposure to chlorpyrifos and bifenthrin: chlorpyrifos combination 1:3 the AchE activity was increased 45.24 and 64.28%, respectively. This increase was much more prominent in the 6th instar larvae viz., 93.95, 45.57, 182.52 and 132.61% following treatments with bifenthrin, chlorpyrifos, 3:1 and 1:3 combinations of two insecticides, respectively. In the resistant population this increase was 35.71, 48.10 and 75.61% in the 4th instar, respectively and 9.47, 28.17 and 60.29% in the 6th instar after chlorpyrifos, 3:1 combination and 1:3 combination treatments, respectively. This activity was decreased 63.99% in the 6th instar but increased in the 4th instar 13.36% after bifenthrin exposure. Arylesterase activity was declined after exposure to bifenthrin, chlorpyrifos and 3:1 combination in both the 4th instar(89.47, 64.33 and 36.65%, respectively) and 6th instar(84.49, 54.01, 4.28%, respectively) of Lab-S as well as in the 4th instar(77.73, 55.81 and 35.96%, respectively) of resistant population. This activity was upregulated(84.92, 141.86 and 192.88%, respectively) in the 6th instar of resistant population, following exposures to bifenthrin, chlorpyrifos and 3:1 combination. Whereas, this activity was increased in the 4th instar(53.22%) and 6th instar(124.60%) of Lab-S population as well as in the 4th instar(35.19%) and 6th instar(234.75%) of resistant population after treatment with 1:3 combination, respectively. The findings of current investigation suggests that insects upregulate the synthesis of esterases in general cholinesterase and carboxylesterase in response to exposure to insecticide to combat with the stressful conditions of exposure to toxic chemicals. The activity of the arylesterase, on the contrary is inhibited to variable extent after exposure to the insecticides.</abstract><cop>Lahore</cop><pub>Knowledge Bylanes</pub><doi>10.17582/journal.pjz/20200501083425</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Pakistan journal of zoology, 2020-12, Vol.52 (6), p.2161 |
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| subjects | Acetylcholinesterase Arylesterase Beetles Carboxylesterase Chemical activity Chlorpyrifos Cholinesterase Deltamethrin Endangered & extinct species Esterases Exposure Grain storage Health aspects Insecticide resistance Insecticides Insects Larvae Mortality Pest resistance Pesticides Pests Population Pyrethroids Trogoderma granarium Variance analysis |
| title | Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium |
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