High DNA integrity sperm selection using surface acoustic waves
Male infertility is a global reproductive issue, several clinical approaches have been developed to tackle it, but their effectiveness is limited by the labour-intensive and time-consuming sperm selection procedures used. Here, we present an automated, acoustic based continuous-flow method capable o...
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| Veröffentlicht in: | Lab on a chip 2020-11, Vol.2 (22), p.4262-4272 |
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| creator | Gai, Junyang Nosrati, Reza Neild, Adrian |
| description | Male infertility is a global reproductive issue, several clinical approaches have been developed to tackle it, but their effectiveness is limited by the labour-intensive and time-consuming sperm selection procedures used. Here, we present an automated, acoustic based continuous-flow method capable of selecting high quality sperm with considerably improved motility and DNA integrity compared to the initial raw bull semen. The acoustic field translates larger sperm and guides highly motile sperm across the channel width. The result is the selection of sperm with over 50% and 60% improvement in vitality and progressive motility and more than 38% improvement in DNA integrity, respectively, while providing a clinically relevant volume and selected sperm number for the performance of
in vitro
fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) by selecting over 60 000 sperm in under an hour.
We presented an automated option for motility-based sperm selection with the potential to sort based on morphology with surface acoustic wave microfluidics. |
| doi_str_mv | 10.1039/d0lc00457j |
| format | Article |
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in vitro
fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) by selecting over 60 000 sperm in under an hour.
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in vitro
fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) by selecting over 60 000 sperm in under an hour.
We presented an automated option for motility-based sperm selection with the potential to sort based on morphology with surface acoustic wave microfluidics.</description><subject>Acoustics</subject><subject>Biochemical Research Methods</subject><subject>Biochemistry & Molecular Biology</subject><subject>Chemistry</subject><subject>Chemistry, Analytical</subject><subject>Chemistry, Multidisciplinary</subject><subject>Continuous flow</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>In vitro fertilization</subject><subject>Infertility</subject><subject>Instruments & Instrumentation</subject><subject>Integrity</subject><subject>Life Sciences & Biomedicine</subject><subject>Motility</subject><subject>Nanoscience & Nanotechnology</subject><subject>Physical Sciences</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Semen</subject><subject>Sperm</subject><subject>Surface acoustic waves</subject><subject>Technology</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqN0btPwzAQBnALgaA8FnakSCwIVDg_EtsTqsKjoAoWmCPHuRRXaVLshIr_npSiIjEx-YbfZ52-I-SYwiUFrq8KqCyAiOVsiwyokHwIVOntzazlHtkPYQZAY5GoXbLHOUjOpBiQ67GbvkU3T6PI1S1OvWs_o7BAP48CVmhb19RRF1w9jULnS2MxMrbpQutstDQfGA7JTmmqgEc_7wF5vbt9ScfDyfP9QzqaDC3XrB2i5lAkNGZaoi4U0pzlcc4LSIS0WoIsZWKlMIontgSR5NwC0woLyeMCTM4PyNn634Vv3jsMbTZ3wWJVmRr7fTImYgY6EZT29PQPnTWdr_vtVkppoYGt1PlaWd-E4LHMFt7Njf_MKGSrWrMbmKTftT72-GKNl5g3ZbAOa4ubAADESlHNVD9R3mv1f5261qxaTpuubvvoyTrqg90kfu_LvwDayZEk</recordid><startdate>20201110</startdate><enddate>20201110</enddate><creator>Gai, Junyang</creator><creator>Nosrati, Reza</creator><creator>Neild, Adrian</creator><general>Royal Soc Chemistry</general><general>Royal Society of Chemistry</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1461-229X</orcidid><orcidid>https://orcid.org/0000-0002-7571-2526</orcidid></search><sort><creationdate>20201110</creationdate><title>High DNA integrity sperm selection using surface acoustic waves</title><author>Gai, Junyang ; Nosrati, Reza ; Neild, Adrian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-e930d615297e9d8e1b2b5b3d0647c9707f76c74a836cf046b3c0298ed735d0ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acoustics</topic><topic>Biochemical Research Methods</topic><topic>Biochemistry & Molecular Biology</topic><topic>Chemistry</topic><topic>Chemistry, Analytical</topic><topic>Chemistry, Multidisciplinary</topic><topic>Continuous flow</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>In vitro fertilization</topic><topic>Infertility</topic><topic>Instruments & Instrumentation</topic><topic>Integrity</topic><topic>Life Sciences & Biomedicine</topic><topic>Motility</topic><topic>Nanoscience & Nanotechnology</topic><topic>Physical Sciences</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Semen</topic><topic>Sperm</topic><topic>Surface acoustic waves</topic><topic>Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gai, Junyang</creatorcontrib><creatorcontrib>Nosrati, Reza</creatorcontrib><creatorcontrib>Neild, Adrian</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gai, Junyang</au><au>Nosrati, Reza</au><au>Neild, Adrian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High DNA integrity sperm selection using surface acoustic waves</atitle><jtitle>Lab on a chip</jtitle><stitle>LAB CHIP</stitle><date>2020-11-10</date><risdate>2020</risdate><volume>2</volume><issue>22</issue><spage>4262</spage><epage>4272</epage><pages>4262-4272</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>Male infertility is a global reproductive issue, several clinical approaches have been developed to tackle it, but their effectiveness is limited by the labour-intensive and time-consuming sperm selection procedures used. Here, we present an automated, acoustic based continuous-flow method capable of selecting high quality sperm with considerably improved motility and DNA integrity compared to the initial raw bull semen. The acoustic field translates larger sperm and guides highly motile sperm across the channel width. The result is the selection of sperm with over 50% and 60% improvement in vitality and progressive motility and more than 38% improvement in DNA integrity, respectively, while providing a clinically relevant volume and selected sperm number for the performance of
in vitro
fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) by selecting over 60 000 sperm in under an hour.
We presented an automated option for motility-based sperm selection with the potential to sort based on morphology with surface acoustic wave microfluidics.</abstract><cop>CAMBRIDGE</cop><pub>Royal Soc Chemistry</pub><pmid>33073274</pmid><doi>10.1039/d0lc00457j</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1461-229X</orcidid><orcidid>https://orcid.org/0000-0002-7571-2526</orcidid></addata></record> |
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| subjects | Acoustics Biochemical Research Methods Biochemistry & Molecular Biology Chemistry Chemistry, Analytical Chemistry, Multidisciplinary Continuous flow Deoxyribonucleic acid DNA In vitro fertilization Infertility Instruments & Instrumentation Integrity Life Sciences & Biomedicine Motility Nanoscience & Nanotechnology Physical Sciences Science & Technology Science & Technology - Other Topics Semen Sperm Surface acoustic waves Technology |
| title | High DNA integrity sperm selection using surface acoustic waves |
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