Effects of stocking density on growth, serum parameters, antioxidant status, liver and intestine histology and gene expression of largemouth bass (Micropterus salmoides) farmed in the in‐pond raceway system
In China, the in‐pond raceway system (IPRS) recirculating culture is a new model of freshwater aquaculture. A 120‐day feeding study was carried out to evaluate the influence of stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology...
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| Veröffentlicht in: | Aquaculture research 2020-12, Vol.51 (12), p.5228-5240 |
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| creator | Wang, Yuyu Ni, Jinjin Nie, Zhijuan Gao, Jiancao Sun, Yi Shao, Nailin Li, Quanjie Hu, Jiawen Xu, Pao Xu, Gangchun |
| description | In China, the in‐pond raceway system (IPRS) recirculating culture is a new model of freshwater aquaculture. A 120‐day feeding study was carried out to evaluate the influence of stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology and gene expression of HSP70 and Cu/Zn‐SOD of largemouth bass farmed in IPRS. Largemouth bass, Micropterus salmoides (initial body weight, 4.50 ± 0.23 g), were randomly farmed in nine raceways stocked at three stocking densities of 0.2 (LSD), 0.4 (MSD) and 0.6 (HSD) kg/m3, and sampled at 30, 60, 90 and 120 days. The results showed that body weight, specific growth rate and weight gain of fish farmed at MSD group were higher than that farmed at LSD and HSD treatments on 30 days (p |
| doi_str_mv | 10.1111/are.14862 |
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A 120‐day feeding study was carried out to evaluate the influence of stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology and gene expression of HSP70 and Cu/Zn‐SOD of largemouth bass farmed in IPRS. Largemouth bass, Micropterus salmoides (initial body weight, 4.50 ± 0.23 g), were randomly farmed in nine raceways stocked at three stocking densities of 0.2 (LSD), 0.4 (MSD) and 0.6 (HSD) kg/m3, and sampled at 30, 60, 90 and 120 days. The results showed that body weight, specific growth rate and weight gain of fish farmed at MSD group were higher than that farmed at LSD and HSD treatments on 30 days (p < 0.05), while fish farmed at MSD and HSD treatments had significantly lower growth than those farmed at LSD groups on 90 and 120 days (p < 0.05). There were no significant differences in serum cortisol, glucose and triglyceride levels among three groups on 30, 60 and 90 days (p > 0.05), whereas fish farmed at HSD group had significant higher values than that farmed at LSD group on 120 day (p < 0.05). There were no significant differences in serum lysozyme level on day 30, while it decreased with increasing stocking densities on after day 60. Fish reared at LSD group had significantly higher SOD activities than those farmed at MSD and HSD treatments, and had significantly higher CAT activities than those farmed at HSD group on 120 day (p < 0.05). MDA level increased with increasing stocking density on days 60, 90 and 120 (p < 0.05). Fish farmed at HSD group had significantly higher liver HSP70 mRNA levels on days 90 and 120 (p < 0.05). Cu/Zn‐SOD mRNA levels showed a downward trend. No significant difference was observed on liver and intestine histology (p > 0.05). In conclusion, largemouth bass farmed at 0.4 kg/m3 had better growth and health status before days 30. After that, fish farmed at higher stocking densities in IPRS would inhibit growth and reduce immune function.]]></description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.14862</identifier><language>eng</language><publisher>Oxford: Hindawi Limited</publisher><subject>antioxidant status ; Antioxidants ; Aquaculture ; Bass ; Body weight ; Density ; Digestive enzymes ; Fish ; Fish farms ; Fish stocking ; Freshwater ; Freshwater aquaculture ; Freshwater fishes ; Gene expression ; growth ; Growth rate ; Histology ; Hormones ; Hsp70 protein ; Immune response ; Inland water environment ; Intestine ; Intestines ; Liver ; Lysozyme ; Micropterus salmoides ; Parameters ; Ponds ; Raceway culture ; Restocking ; Serum ; Stocking ; Stocking density ; Superoxide dismutase ; Triglycerides ; Zinc</subject><ispartof>Aquaculture research, 2020-12, Vol.51 (12), p.5228-5240</ispartof><rights>2020 John Wiley & Sons Ltd</rights><rights>Copyright © 2020 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3322-8f8146f97cb9a03440d8a06acb7b3c2adf4afd92e09a60e157785026161385ce3</citedby><cites>FETCH-LOGICAL-c3322-8f8146f97cb9a03440d8a06acb7b3c2adf4afd92e09a60e157785026161385ce3</cites><orcidid>0000-0003-1667-431X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.14862$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.14862$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Wang, Yuyu</creatorcontrib><creatorcontrib>Ni, Jinjin</creatorcontrib><creatorcontrib>Nie, Zhijuan</creatorcontrib><creatorcontrib>Gao, Jiancao</creatorcontrib><creatorcontrib>Sun, Yi</creatorcontrib><creatorcontrib>Shao, Nailin</creatorcontrib><creatorcontrib>Li, Quanjie</creatorcontrib><creatorcontrib>Hu, Jiawen</creatorcontrib><creatorcontrib>Xu, Pao</creatorcontrib><creatorcontrib>Xu, Gangchun</creatorcontrib><title>Effects of stocking density on growth, serum parameters, antioxidant status, liver and intestine histology and gene expression of largemouth bass (Micropterus salmoides) farmed in the in‐pond raceway system</title><title>Aquaculture research</title><description><![CDATA[In China, the in‐pond raceway system (IPRS) recirculating culture is a new model of freshwater aquaculture. A 120‐day feeding study was carried out to evaluate the influence of stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology and gene expression of HSP70 and Cu/Zn‐SOD of largemouth bass farmed in IPRS. Largemouth bass, Micropterus salmoides (initial body weight, 4.50 ± 0.23 g), were randomly farmed in nine raceways stocked at three stocking densities of 0.2 (LSD), 0.4 (MSD) and 0.6 (HSD) kg/m3, and sampled at 30, 60, 90 and 120 days. The results showed that body weight, specific growth rate and weight gain of fish farmed at MSD group were higher than that farmed at LSD and HSD treatments on 30 days (p < 0.05), while fish farmed at MSD and HSD treatments had significantly lower growth than those farmed at LSD groups on 90 and 120 days (p < 0.05). There were no significant differences in serum cortisol, glucose and triglyceride levels among three groups on 30, 60 and 90 days (p > 0.05), whereas fish farmed at HSD group had significant higher values than that farmed at LSD group on 120 day (p < 0.05). There were no significant differences in serum lysozyme level on day 30, while it decreased with increasing stocking densities on after day 60. Fish reared at LSD group had significantly higher SOD activities than those farmed at MSD and HSD treatments, and had significantly higher CAT activities than those farmed at HSD group on 120 day (p < 0.05). MDA level increased with increasing stocking density on days 60, 90 and 120 (p < 0.05). Fish farmed at HSD group had significantly higher liver HSP70 mRNA levels on days 90 and 120 (p < 0.05). Cu/Zn‐SOD mRNA levels showed a downward trend. No significant difference was observed on liver and intestine histology (p > 0.05). In conclusion, largemouth bass farmed at 0.4 kg/m3 had better growth and health status before days 30. After that, fish farmed at higher stocking densities in IPRS would inhibit growth and reduce immune function.]]></description><subject>antioxidant status</subject><subject>Antioxidants</subject><subject>Aquaculture</subject><subject>Bass</subject><subject>Body weight</subject><subject>Density</subject><subject>Digestive enzymes</subject><subject>Fish</subject><subject>Fish farms</subject><subject>Fish stocking</subject><subject>Freshwater</subject><subject>Freshwater aquaculture</subject><subject>Freshwater fishes</subject><subject>Gene expression</subject><subject>growth</subject><subject>Growth rate</subject><subject>Histology</subject><subject>Hormones</subject><subject>Hsp70 protein</subject><subject>Immune response</subject><subject>Inland water environment</subject><subject>Intestine</subject><subject>Intestines</subject><subject>Liver</subject><subject>Lysozyme</subject><subject>Micropterus salmoides</subject><subject>Parameters</subject><subject>Ponds</subject><subject>Raceway culture</subject><subject>Restocking</subject><subject>Serum</subject><subject>Stocking</subject><subject>Stocking density</subject><subject>Superoxide dismutase</subject><subject>Triglycerides</subject><subject>Zinc</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1Uctu1EAQtBBIhMCBPxiJC5HiZMb2-HGMooUgBSEhkLhZveMe7wTbY6bHbHzjE_JpfANfQm-WK3OpUXV1lVqVJK-VvFD8LiHghSrqMnuSnKi81GmmZPP08Nc61br69jx5QXQnpSpkrk6S3xtr0UQS3gqK3nx3Uy86nMjFVfhJ9MHv4-5cEIZlFDMEGDFioHMBU3T-3nWMvAlxYW5wPzHwpBNuikjRTSh2jn0H36-PfI9M4f0ckMixP8cOEHoc_RJ3YgtE4u1HZ4KfOWUhQTCM3nVIZ8JCGPHgLOIOGf78epg9WwYwuIdV0EoRx5fJMwsD4at_eJp8fbf5cn2T3n56_-H66jY1eZ5laW1rVZS2qcy2AZkXhexqkCWYbbXNTQadLcB2TYaygVKi0lVVa5mVqlR5rQ3mp8mbo-8c_I-Fb23v_BImjmyzQtdVWUlds-rsqOKLiALadg5uhLC2SraHwlourH0sjLWXR-3eDbj-X9hefd4cN_4CKgadyA</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>Wang, Yuyu</creator><creator>Ni, Jinjin</creator><creator>Nie, Zhijuan</creator><creator>Gao, Jiancao</creator><creator>Sun, Yi</creator><creator>Shao, Nailin</creator><creator>Li, Quanjie</creator><creator>Hu, Jiawen</creator><creator>Xu, Pao</creator><creator>Xu, Gangchun</creator><general>Hindawi Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-1667-431X</orcidid></search><sort><creationdate>202012</creationdate><title>Effects of stocking density on growth, serum parameters, antioxidant status, liver and intestine histology and gene expression of largemouth bass (Micropterus salmoides) farmed in the in‐pond raceway system</title><author>Wang, Yuyu ; Ni, Jinjin ; Nie, Zhijuan ; Gao, Jiancao ; Sun, Yi ; Shao, Nailin ; Li, Quanjie ; Hu, Jiawen ; Xu, Pao ; Xu, Gangchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3322-8f8146f97cb9a03440d8a06acb7b3c2adf4afd92e09a60e157785026161385ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>antioxidant status</topic><topic>Antioxidants</topic><topic>Aquaculture</topic><topic>Bass</topic><topic>Body weight</topic><topic>Density</topic><topic>Digestive enzymes</topic><topic>Fish</topic><topic>Fish farms</topic><topic>Fish stocking</topic><topic>Freshwater</topic><topic>Freshwater aquaculture</topic><topic>Freshwater fishes</topic><topic>Gene expression</topic><topic>growth</topic><topic>Growth rate</topic><topic>Histology</topic><topic>Hormones</topic><topic>Hsp70 protein</topic><topic>Immune response</topic><topic>Inland water environment</topic><topic>Intestine</topic><topic>Intestines</topic><topic>Liver</topic><topic>Lysozyme</topic><topic>Micropterus salmoides</topic><topic>Parameters</topic><topic>Ponds</topic><topic>Raceway culture</topic><topic>Restocking</topic><topic>Serum</topic><topic>Stocking</topic><topic>Stocking density</topic><topic>Superoxide dismutase</topic><topic>Triglycerides</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yuyu</creatorcontrib><creatorcontrib>Ni, Jinjin</creatorcontrib><creatorcontrib>Nie, Zhijuan</creatorcontrib><creatorcontrib>Gao, Jiancao</creatorcontrib><creatorcontrib>Sun, Yi</creatorcontrib><creatorcontrib>Shao, Nailin</creatorcontrib><creatorcontrib>Li, Quanjie</creatorcontrib><creatorcontrib>Hu, Jiawen</creatorcontrib><creatorcontrib>Xu, Pao</creatorcontrib><creatorcontrib>Xu, Gangchun</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yuyu</au><au>Ni, Jinjin</au><au>Nie, Zhijuan</au><au>Gao, Jiancao</au><au>Sun, Yi</au><au>Shao, Nailin</au><au>Li, Quanjie</au><au>Hu, Jiawen</au><au>Xu, Pao</au><au>Xu, Gangchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of stocking density on growth, serum parameters, antioxidant status, liver and intestine histology and gene expression of largemouth bass (Micropterus salmoides) farmed in the in‐pond raceway system</atitle><jtitle>Aquaculture research</jtitle><date>2020-12</date><risdate>2020</risdate><volume>51</volume><issue>12</issue><spage>5228</spage><epage>5240</epage><pages>5228-5240</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract><![CDATA[In China, the in‐pond raceway system (IPRS) recirculating culture is a new model of freshwater aquaculture. A 120‐day feeding study was carried out to evaluate the influence of stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology and gene expression of HSP70 and Cu/Zn‐SOD of largemouth bass farmed in IPRS. Largemouth bass, Micropterus salmoides (initial body weight, 4.50 ± 0.23 g), were randomly farmed in nine raceways stocked at three stocking densities of 0.2 (LSD), 0.4 (MSD) and 0.6 (HSD) kg/m3, and sampled at 30, 60, 90 and 120 days. The results showed that body weight, specific growth rate and weight gain of fish farmed at MSD group were higher than that farmed at LSD and HSD treatments on 30 days (p < 0.05), while fish farmed at MSD and HSD treatments had significantly lower growth than those farmed at LSD groups on 90 and 120 days (p < 0.05). There were no significant differences in serum cortisol, glucose and triglyceride levels among three groups on 30, 60 and 90 days (p > 0.05), whereas fish farmed at HSD group had significant higher values than that farmed at LSD group on 120 day (p < 0.05). There were no significant differences in serum lysozyme level on day 30, while it decreased with increasing stocking densities on after day 60. Fish reared at LSD group had significantly higher SOD activities than those farmed at MSD and HSD treatments, and had significantly higher CAT activities than those farmed at HSD group on 120 day (p < 0.05). MDA level increased with increasing stocking density on days 60, 90 and 120 (p < 0.05). Fish farmed at HSD group had significantly higher liver HSP70 mRNA levels on days 90 and 120 (p < 0.05). Cu/Zn‐SOD mRNA levels showed a downward trend. No significant difference was observed on liver and intestine histology (p > 0.05). In conclusion, largemouth bass farmed at 0.4 kg/m3 had better growth and health status before days 30. After that, fish farmed at higher stocking densities in IPRS would inhibit growth and reduce immune function.]]></abstract><cop>Oxford</cop><pub>Hindawi Limited</pub><doi>10.1111/are.14862</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-1667-431X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | antioxidant status Antioxidants Aquaculture Bass Body weight Density Digestive enzymes Fish Fish farms Fish stocking Freshwater Freshwater aquaculture Freshwater fishes Gene expression growth Growth rate Histology Hormones Hsp70 protein Immune response Inland water environment Intestine Intestines Liver Lysozyme Micropterus salmoides Parameters Ponds Raceway culture Restocking Serum Stocking Stocking density Superoxide dismutase Triglycerides Zinc |
| title | Effects of stocking density on growth, serum parameters, antioxidant status, liver and intestine histology and gene expression of largemouth bass (Micropterus salmoides) farmed in the in‐pond raceway system |
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