In Cellulo Protein Semi‐Synthesis from Endogenous and Exogenous Fragments Using the Ultra‐Fast Split Gp41‐1 Intein

Protein semi‐synthesis inside live cells from exogenous and endogenous parts offers unique possibilities for studying proteins in their native context. Split‐intein‐mediated protein trans‐splicing is predestined for such endeavors and has seen some successes, but a much larger variety of established...

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Veröffentlicht in:	Angewandte Chemie 2020-11, Vol.132 (47), p.21193-21201
Hauptverfasser:	Bhagawati, Maniraj, Hoffmann, Simon, Höffgen, Katharina S., Piehler, Jacob, Busch, Karin B., Mootz, Henning D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry dSTORM Entrapment Fluorescence Fragments Glycoprotein gp41 Inteins Mammalian cells Mitochondria Particle tracking Perturbation Protein biosynthesis protein splicing protein transduction Proteins Semisynthesis single-molecule studies Splicing split intein Structure-function relationships Synthesis
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