Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes
Summary Plant‐based oils are valuable agricultural products, and seed oil content (SOC) is the major yield component in oil crops. Increasing SOC has been successfully targeted through the selection and genetic modification of oil biosynthesis. The SOC in rapeseed declined during the seed maturation...
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| Veröffentlicht in: | Plant biotechnology journal 2020-11, Vol.18 (11), p.2251-2266 |
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| creator | Karunarathna, Nirosha L. Wang, Haoyi Harloff, Hans‐Joachim Jiang, Lixi Jung, Christian |
| description | Summary
Plant‐based oils are valuable agricultural products, and seed oil content (SOC) is the major yield component in oil crops. Increasing SOC has been successfully targeted through the selection and genetic modification of oil biosynthesis. The SOC in rapeseed declined during the seed maturation and eventually caused the final accumulated seed oil quantity. However, genes involved in oil degradation during seed maturity are not deeply studied so far. We performed a candidate gene association study using a worldwide collection of rapeseed germplasm. We identified SEED FATTY ACID REDUCER (SFAR) genes, which had a significant effect on SOC and fatty acid (FA) composition. SFAR genes belong to the GDSL lipases, and GDSL lipases have a broad range of functions in plants. After quantification of gene expression using RNA‐seq and quantitative PCR, we used targeted (CRISPR‐Cas mediated) and random (chemical) mutagenesis to modify turnover rates of seed oil in winter rapeseed. For the first time, we demonstrate significant increase of SOC in a crop after knocking out members of the BnSFAR4 and BnSFAR5 gene families without pleiotropic effects on seed germination, vigour and oil mobilization. Our results offer new perspectives for improving oil yield by targeted mutagenesis. |
| doi_str_mv | 10.1111/pbi.13381 |
| format | Article |
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Plant‐based oils are valuable agricultural products, and seed oil content (SOC) is the major yield component in oil crops. Increasing SOC has been successfully targeted through the selection and genetic modification of oil biosynthesis. The SOC in rapeseed declined during the seed maturation and eventually caused the final accumulated seed oil quantity. However, genes involved in oil degradation during seed maturity are not deeply studied so far. We performed a candidate gene association study using a worldwide collection of rapeseed germplasm. We identified SEED FATTY ACID REDUCER (SFAR) genes, which had a significant effect on SOC and fatty acid (FA) composition. SFAR genes belong to the GDSL lipases, and GDSL lipases have a broad range of functions in plants. After quantification of gene expression using RNA‐seq and quantitative PCR, we used targeted (CRISPR‐Cas mediated) and random (chemical) mutagenesis to modify turnover rates of seed oil in winter rapeseed. For the first time, we demonstrate significant increase of SOC in a crop after knocking out members of the BnSFAR4 and BnSFAR5 gene families without pleiotropic effects on seed germination, vigour and oil mobilization. Our results offer new perspectives for improving oil yield by targeted mutagenesis.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/pbi.13381</identifier><identifier>PMID: 32216029</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Agricultural products ; Biosynthesis ; Biotechnology & Applied Microbiology ; Brassica napus ; Brassica napus - genetics ; CRISPR ; CRISPR‐Cas ; Crops ; EMS ; Fatty Acids ; GDSL ; Gene expression ; Gene families ; Genes ; Genetic modification ; genome editing ; Genomes ; Germination ; Germplasm ; Humans ; Life Sciences & Biomedicine ; Lipids ; Mutagenesis ; Mutation ; Oils & fats ; Oilseeds ; Phylogenetics ; Plant Oils ; Plant Sciences ; Polyploidy ; Rapeseed ; Ribonucleic acid ; RNA ; Science & Technology ; Seed germination ; Seeds ; Seeds - genetics ; SFAR ; Site-directed mutagenesis</subject><ispartof>Plant biotechnology journal, 2020-11, Vol.18 (11), p.2251-2266</ispartof><rights>2020 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>61</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000525990800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c4831-a1f33aa1dd066cd1fa616fbe8b64095abad5afa1fc733b9aff4659bd4d3a60d83</citedby><cites>FETCH-LOGICAL-c4831-a1f33aa1dd066cd1fa616fbe8b64095abad5afa1fc733b9aff4659bd4d3a60d83</cites><orcidid>0000-0001-8149-7976 ; 0000-0002-1844-7156</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpbi.13381$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpbi.13381$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,865,886,1418,2115,11567,27929,27930,28253,45579,45580,46057,46481</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32216029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karunarathna, Nirosha L.</creatorcontrib><creatorcontrib>Wang, Haoyi</creatorcontrib><creatorcontrib>Harloff, Hans‐Joachim</creatorcontrib><creatorcontrib>Jiang, Lixi</creatorcontrib><creatorcontrib>Jung, Christian</creatorcontrib><title>Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes</title><title>Plant biotechnology journal</title><addtitle>PLANT BIOTECHNOL J</addtitle><addtitle>Plant Biotechnol J</addtitle><description>Summary
Plant‐based oils are valuable agricultural products, and seed oil content (SOC) is the major yield component in oil crops. Increasing SOC has been successfully targeted through the selection and genetic modification of oil biosynthesis. The SOC in rapeseed declined during the seed maturation and eventually caused the final accumulated seed oil quantity. However, genes involved in oil degradation during seed maturity are not deeply studied so far. We performed a candidate gene association study using a worldwide collection of rapeseed germplasm. We identified SEED FATTY ACID REDUCER (SFAR) genes, which had a significant effect on SOC and fatty acid (FA) composition. SFAR genes belong to the GDSL lipases, and GDSL lipases have a broad range of functions in plants. After quantification of gene expression using RNA‐seq and quantitative PCR, we used targeted (CRISPR‐Cas mediated) and random (chemical) mutagenesis to modify turnover rates of seed oil in winter rapeseed. For the first time, we demonstrate significant increase of SOC in a crop after knocking out members of the BnSFAR4 and BnSFAR5 gene families without pleiotropic effects on seed germination, vigour and oil mobilization. Our results offer new perspectives for improving oil yield by targeted mutagenesis.</description><subject>Agricultural products</subject><subject>Biosynthesis</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Brassica napus</subject><subject>Brassica napus - genetics</subject><subject>CRISPR</subject><subject>CRISPR‐Cas</subject><subject>Crops</subject><subject>EMS</subject><subject>Fatty Acids</subject><subject>GDSL</subject><subject>Gene expression</subject><subject>Gene families</subject><subject>Genes</subject><subject>Genetic modification</subject><subject>genome editing</subject><subject>Genomes</subject><subject>Germination</subject><subject>Germplasm</subject><subject>Humans</subject><subject>Life Sciences & Biomedicine</subject><subject>Lipids</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Oils & fats</subject><subject>Oilseeds</subject><subject>Phylogenetics</subject><subject>Plant Oils</subject><subject>Plant Sciences</subject><subject>Polyploidy</subject><subject>Rapeseed</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Science & Technology</subject><subject>Seed germination</subject><subject>Seeds</subject><subject>Seeds - genetics</subject><subject>SFAR</subject><subject>Site-directed mutagenesis</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkUtv1DAUhS0EoqV0wR9AllhVaFo7fkyyQRoyaRmpEqidLlhZfg6uMnaIk1bz7-uSYQQLJLzxlf3d4-N7AHiH0TnO66JT_hwTUuIX4BhTPp_NOSteHmpKj8CblO4RKjBn_DU4IkWuUFEdA9O09kEOPmxgstbA6FuoYxhsGKAPUMIutruujd5A3ccOql0-NqPO6HYccmMM6Rm8bZolvFys19_hol4t4U2zvKubG7ixwaa34JWTbbKn-_0E3F026_rL7Prr1apeXM80LQmeSewIkRIbgzjXBjvJMXfKlopTVDGppGHSZUrPCVGVdI5yVilDDZEcmZKcgE-TbjeqrTU6f6KXreh6v5X9TkTpxd83wf8Qm_gg5qysCsaywIe9QB9_jjYN4j6OfcieRUEZJZiTqsjU2UTliaTUW3d4ASPxHIjIgYhfgWT2_Z-WDuTvBDJQTsCjVdEl7W3Q9oAhhFjBqgqVuUK49tPI6ziGIbd-_P_WTF_sad_a3b8ti2-fV5P3J9W8tbw</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Karunarathna, Nirosha L.</creator><creator>Wang, Haoyi</creator><creator>Harloff, Hans‐Joachim</creator><creator>Jiang, Lixi</creator><creator>Jung, Christian</creator><general>Wiley</general><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8149-7976</orcidid><orcidid>https://orcid.org/0000-0002-1844-7156</orcidid></search><sort><creationdate>202011</creationdate><title>Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes</title><author>Karunarathna, Nirosha L. ; 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Plant‐based oils are valuable agricultural products, and seed oil content (SOC) is the major yield component in oil crops. Increasing SOC has been successfully targeted through the selection and genetic modification of oil biosynthesis. The SOC in rapeseed declined during the seed maturation and eventually caused the final accumulated seed oil quantity. However, genes involved in oil degradation during seed maturity are not deeply studied so far. We performed a candidate gene association study using a worldwide collection of rapeseed germplasm. We identified SEED FATTY ACID REDUCER (SFAR) genes, which had a significant effect on SOC and fatty acid (FA) composition. SFAR genes belong to the GDSL lipases, and GDSL lipases have a broad range of functions in plants. After quantification of gene expression using RNA‐seq and quantitative PCR, we used targeted (CRISPR‐Cas mediated) and random (chemical) mutagenesis to modify turnover rates of seed oil in winter rapeseed. For the first time, we demonstrate significant increase of SOC in a crop after knocking out members of the BnSFAR4 and BnSFAR5 gene families without pleiotropic effects on seed germination, vigour and oil mobilization. Our results offer new perspectives for improving oil yield by targeted mutagenesis.</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>32216029</pmid><doi>10.1111/pbi.13381</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-8149-7976</orcidid><orcidid>https://orcid.org/0000-0002-1844-7156</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Agricultural products Biosynthesis Biotechnology & Applied Microbiology Brassica napus Brassica napus - genetics CRISPR CRISPR‐Cas Crops EMS Fatty Acids GDSL Gene expression Gene families Genes Genetic modification genome editing Genomes Germination Germplasm Humans Life Sciences & Biomedicine Lipids Mutagenesis Mutation Oils & fats Oilseeds Phylogenetics Plant Oils Plant Sciences Polyploidy Rapeseed Ribonucleic acid RNA Science & Technology Seed germination Seeds Seeds - genetics SFAR Site-directed mutagenesis |
| title | Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes |
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