Overcoming multimeric target interference in a bridging immunogenicity assay with soluble target receptor, target immunodepletion and mild acidic assay pH

Soluble multimeric target proteins can generate a target-mediated false-positive signal in bridging anti-drug antibody (ADA) assays. A high background signal due to target interference was observed in our anti-REGN-Y antibody assay, and two different strategies were evaluated to mitigate this false-...

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Veröffentlicht in:	Bioanalysis 2020-08, Vol.12 (15), p.1071-1085
Hauptverfasser:	Chen, Jihua, Kendra, Kimberly, Torri, Albert, Sumner, Giane
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids anti-drug antibody Antibodies Binding sites Biological Assay - methods co-factor Humans Hydrogen-Ion Concentration Immunogenetics - methods Immunogenicity Immunological tolerance mild acidic assay pH pH effects Polyclonal antibodies Proteins soluble target receptor target immunodepletion target interference
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