Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma
Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of...
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| Veröffentlicht in: | Plasma chemistry and plasma processing 2020-11, Vol.40 (6), p.1571-1584 |
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| creator | Tomeková, J. Kyzek, S. Medvecká, V. Gálová, E. Zahoranová, A. |
| description | Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (
Pisum sativum
L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air. |
| doi_str_mv | 10.1007/s11090-020-10109-8 |
| format | Article |
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Pisum sativum
L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air.</description><identifier>ISSN: 0272-4324</identifier><identifier>EISSN: 1572-8986</identifier><identifier>DOI: 10.1007/s11090-020-10109-8</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Air plasma ; Atmospheric pressure ; Bioassays ; Characterization and Evaluation of Materials ; Chemical composition ; Chemistry ; Chemistry and Materials Science ; Classical Mechanics ; Damage detection ; Deoxyribonucleic acid ; DNA ; DNA damage ; Electrophoresis ; Emission analysis ; Fourier transforms ; Germination ; Inorganic Chemistry ; Mechanical Engineering ; Nitrogen ; Optical emission spectroscopy ; Original Paper ; Oxygen ; Parameters ; Plasma ; Plasma composition ; Plasma radiation ; Reaction mechanisms ; Seeds ; Spectrum analysis ; Water vapor</subject><ispartof>Plasma chemistry and plasma processing, 2020-11, Vol.40 (6), p.1571-1584</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-8deccb1d691bfb82b0abbbea278b350dfc68b3193d1190034e866dffd2d911463</citedby><cites>FETCH-LOGICAL-c385t-8deccb1d691bfb82b0abbbea278b350dfc68b3193d1190034e866dffd2d911463</cites><orcidid>0000-0001-7631-3511 ; 0000-0002-0772-9385</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11090-020-10109-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11090-020-10109-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Tomeková, J.</creatorcontrib><creatorcontrib>Kyzek, S.</creatorcontrib><creatorcontrib>Medvecká, V.</creatorcontrib><creatorcontrib>Gálová, E.</creatorcontrib><creatorcontrib>Zahoranová, A.</creatorcontrib><title>Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma</title><title>Plasma chemistry and plasma processing</title><addtitle>Plasma Chem Plasma Process</addtitle><description>Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (
Pisum sativum
L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air.</description><subject>Air plasma</subject><subject>Atmospheric pressure</subject><subject>Bioassays</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical composition</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Classical Mechanics</subject><subject>Damage detection</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Electrophoresis</subject><subject>Emission analysis</subject><subject>Fourier transforms</subject><subject>Germination</subject><subject>Inorganic Chemistry</subject><subject>Mechanical Engineering</subject><subject>Nitrogen</subject><subject>Optical emission spectroscopy</subject><subject>Original Paper</subject><subject>Oxygen</subject><subject>Parameters</subject><subject>Plasma</subject><subject>Plasma composition</subject><subject>Plasma radiation</subject><subject>Reaction mechanisms</subject><subject>Seeds</subject><subject>Spectrum analysis</subject><subject>Water vapor</subject><issn>0272-4324</issn><issn>1572-8986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMoOI7-AVcB19UkfaXuhhlfMDgD6jrkWTu0SU3ahf_edCq4c3XPDeecSz4ArjG6xQiVdwFjVKEEEZRgFGVCT8AC5yVJaEWLU7BAJOosJdk5uAjhgKIJpeUCjC_WtKO2UkNn4Nq1Cq6GzoX-U_tGwr3XIYxew33LQ8ehs3CvOXzTWoV7uHldwQ3veH0MT49tY-sAuVVw1w-N5C3cNLy2LsQlTKa55xKcGd4GffU7l-Dj8eF9_Zxsd08v69U2kSnNh4QqLaXAqqiwMIISgbgQQnNSUpHmSBlZRIGrVGFcxf9kmhaFMkYRVWGcFekS3My9vXdfow4DO7jR23iSkSxHGUERT3SR2SW9C8Frw3rfdNx_M4zYhJfNeFnEy4542RRK51CIZltr_1f9T-oHrdx9Nw</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Tomeková, J.</creator><creator>Kyzek, S.</creator><creator>Medvecká, V.</creator><creator>Gálová, E.</creator><creator>Zahoranová, A.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-7631-3511</orcidid><orcidid>https://orcid.org/0000-0002-0772-9385</orcidid></search><sort><creationdate>20201101</creationdate><title>Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma</title><author>Tomeková, J. ; Kyzek, S. ; Medvecká, V. ; Gálová, E. ; Zahoranová, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-8deccb1d691bfb82b0abbbea278b350dfc68b3193d1190034e866dffd2d911463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Air plasma</topic><topic>Atmospheric pressure</topic><topic>Bioassays</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical composition</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Classical Mechanics</topic><topic>Damage detection</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Electrophoresis</topic><topic>Emission analysis</topic><topic>Fourier transforms</topic><topic>Germination</topic><topic>Inorganic Chemistry</topic><topic>Mechanical Engineering</topic><topic>Nitrogen</topic><topic>Optical emission spectroscopy</topic><topic>Original Paper</topic><topic>Oxygen</topic><topic>Parameters</topic><topic>Plasma</topic><topic>Plasma composition</topic><topic>Plasma radiation</topic><topic>Reaction mechanisms</topic><topic>Seeds</topic><topic>Spectrum analysis</topic><topic>Water vapor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomeková, J.</creatorcontrib><creatorcontrib>Kyzek, S.</creatorcontrib><creatorcontrib>Medvecká, V.</creatorcontrib><creatorcontrib>Gálová, E.</creatorcontrib><creatorcontrib>Zahoranová, A.</creatorcontrib><collection>CrossRef</collection><jtitle>Plasma chemistry and plasma processing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomeková, J.</au><au>Kyzek, S.</au><au>Medvecká, V.</au><au>Gálová, E.</au><au>Zahoranová, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma</atitle><jtitle>Plasma chemistry and plasma processing</jtitle><stitle>Plasma Chem Plasma Process</stitle><date>2020-11-01</date><risdate>2020</risdate><volume>40</volume><issue>6</issue><spage>1571</spage><epage>1584</epage><pages>1571-1584</pages><issn>0272-4324</issn><eissn>1572-8986</eissn><abstract>Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (
Pisum sativum
L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11090-020-10109-8</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-7631-3511</orcidid><orcidid>https://orcid.org/0000-0002-0772-9385</orcidid></addata></record> |
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| subjects | Air plasma Atmospheric pressure Bioassays Characterization and Evaluation of Materials Chemical composition Chemistry Chemistry and Materials Science Classical Mechanics Damage detection Deoxyribonucleic acid DNA DNA damage Electrophoresis Emission analysis Fourier transforms Germination Inorganic Chemistry Mechanical Engineering Nitrogen Optical emission spectroscopy Original Paper Oxygen Parameters Plasma Plasma composition Plasma radiation Reaction mechanisms Seeds Spectrum analysis Water vapor |
| title | Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma |
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