Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells

A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cell and tissue biology 2020-09, Vol.14 (5), p.349-357
Hauptverfasser: Voronkina, I. V., Smagina, L. V., Bildyug, N. B., Musorina, A. S., Poljanskaya, G. G.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 357
container_issue 5
container_start_page 349
container_title Cell and tissue biology
container_volume 14
creator Voronkina, I. V.
Smagina, L. V.
Bildyug, N. B.
Musorina, A. S.
Poljanskaya, G. G.
description A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matrix metalloproteinases (MMPs). In this work, we compared the dynamics of MMP activity and the level of ECM proteins during replicative senescence of three lines of human MSCs obtained from Wharton’s jelly of human umbilical cord (MSCWJ-1 line), eyelid skin (DF-2 line), and human epicardial adipose tissue isolated during coronary artery bypass grafting (ADH-MSC line). The fractions of cells with β-galactosidase enzyme activity (marker of replicative senescence) and the content of ECM proteins (fibronectin and type I collagen), as well as the activity of MMP-1, MMP-2, and MMP-9, were analyzed during long-term cultivation. It was found that three lines differ in the content of fibronectin, type 1 collagen, and MMP activity during the replicative senescence. Cells of the ADH-MSC line are mostly different from the other two lines in terms of aging rate, ECM protein content, and MMP activity. The reason for this discrepancy is that the cells are obtained from the tissue of a patient having heart disease.
doi_str_mv 10.1134/S1990519X20050107
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2450230783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2450230783</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2317-c3ebda63af0fb6bacf646f45786ed2295eaa02564c54cf9b75ff0678e5d028173</originalsourceid><addsrcrecordid>eNp1Uc1Kw0AQDqJgrT6AtwXP1dlNNmmOpf5UaFGsgrcw3c7alGRTd7fSvI2P6tZaPIinGb5fhomicw6XnMfJ1ZTnOUievwoACRyyg6izhXpSQHy43wN_HJ04twRIIeHQiT6vW4N1qRxrNJugt-WGTchjVTUr23gqDTpiA-XLj9K3DM2c3Wy8RUVVta7Q7j2PO7Fjw8Z4Mp6VhvkFbXFF7jv9iVZVqTAkEZuSIafIKNoyo3WNJtS6ACzaGis29VSzYehwp9GRxsrR2c_sRi-3N8_DUW_8cHc_HIx7SsQ866mYZnNMY9SgZ-kMlU6TVCcy66c0FyKXhAhCpomSidL5LJNaQ5r1Sc5B9HkWd6OLXW44-31NzhfLZm1NqCxEIkHEkPXjoOI7lbKNc5Z0sbJljbYtOBTbRxR_HhE8YudxQWveyP4m_2_6Amfsjcc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2450230783</pqid></control><display><type>article</type><title>Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells</title><source>SpringerLink Journals - AutoHoldings</source><creator>Voronkina, I. V. ; Smagina, L. V. ; Bildyug, N. B. ; Musorina, A. S. ; Poljanskaya, G. G.</creator><creatorcontrib>Voronkina, I. V. ; Smagina, L. V. ; Bildyug, N. B. ; Musorina, A. S. ; Poljanskaya, G. G.</creatorcontrib><description>A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matrix metalloproteinases (MMPs). In this work, we compared the dynamics of MMP activity and the level of ECM proteins during replicative senescence of three lines of human MSCs obtained from Wharton’s jelly of human umbilical cord (MSCWJ-1 line), eyelid skin (DF-2 line), and human epicardial adipose tissue isolated during coronary artery bypass grafting (ADH-MSC line). The fractions of cells with β-galactosidase enzyme activity (marker of replicative senescence) and the content of ECM proteins (fibronectin and type I collagen), as well as the activity of MMP-1, MMP-2, and MMP-9, were analyzed during long-term cultivation. It was found that three lines differ in the content of fibronectin, type 1 collagen, and MMP activity during the replicative senescence. Cells of the ADH-MSC line are mostly different from the other two lines in terms of aging rate, ECM protein content, and MMP activity. The reason for this discrepancy is that the cells are obtained from the tissue of a patient having heart disease.</description><identifier>ISSN: 1990-519X</identifier><identifier>EISSN: 1990-5203</identifier><identifier>DOI: 10.1134/S1990519X20050107</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Adipose tissue ; Aging ; Biomedical and Life Sciences ; Cell Biology ; Collagen (type I) ; Comparative analysis ; Coronary artery ; Coronary artery disease ; Enzymatic activity ; Extracellular matrix ; Eyelid ; Fibronectin ; Gelatinase A ; Gelatinase B ; Heart diseases ; Heart surgery ; Interstitial collagenase ; Life Sciences ; Matrix metalloproteinase ; Mesenchymal stem cells ; Metalloproteinase ; Proteins ; Senescence ; Stem cells ; Umbilical cord ; β-Galactosidase</subject><ispartof>Cell and tissue biology, 2020-09, Vol.14 (5), p.349-357</ispartof><rights>Pleiades Publishing, Ltd. 2020</rights><rights>Pleiades Publishing, Ltd. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2317-c3ebda63af0fb6bacf646f45786ed2295eaa02564c54cf9b75ff0678e5d028173</citedby><cites>FETCH-LOGICAL-c2317-c3ebda63af0fb6bacf646f45786ed2295eaa02564c54cf9b75ff0678e5d028173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1990519X20050107$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1990519X20050107$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Voronkina, I. V.</creatorcontrib><creatorcontrib>Smagina, L. V.</creatorcontrib><creatorcontrib>Bildyug, N. B.</creatorcontrib><creatorcontrib>Musorina, A. S.</creatorcontrib><creatorcontrib>Poljanskaya, G. G.</creatorcontrib><title>Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells</title><title>Cell and tissue biology</title><addtitle>Cell Tiss. Biol</addtitle><description>A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matrix metalloproteinases (MMPs). In this work, we compared the dynamics of MMP activity and the level of ECM proteins during replicative senescence of three lines of human MSCs obtained from Wharton’s jelly of human umbilical cord (MSCWJ-1 line), eyelid skin (DF-2 line), and human epicardial adipose tissue isolated during coronary artery bypass grafting (ADH-MSC line). The fractions of cells with β-galactosidase enzyme activity (marker of replicative senescence) and the content of ECM proteins (fibronectin and type I collagen), as well as the activity of MMP-1, MMP-2, and MMP-9, were analyzed during long-term cultivation. It was found that three lines differ in the content of fibronectin, type 1 collagen, and MMP activity during the replicative senescence. Cells of the ADH-MSC line are mostly different from the other two lines in terms of aging rate, ECM protein content, and MMP activity. The reason for this discrepancy is that the cells are obtained from the tissue of a patient having heart disease.</description><subject>Adipose tissue</subject><subject>Aging</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Collagen (type I)</subject><subject>Comparative analysis</subject><subject>Coronary artery</subject><subject>Coronary artery disease</subject><subject>Enzymatic activity</subject><subject>Extracellular matrix</subject><subject>Eyelid</subject><subject>Fibronectin</subject><subject>Gelatinase A</subject><subject>Gelatinase B</subject><subject>Heart diseases</subject><subject>Heart surgery</subject><subject>Interstitial collagenase</subject><subject>Life Sciences</subject><subject>Matrix metalloproteinase</subject><subject>Mesenchymal stem cells</subject><subject>Metalloproteinase</subject><subject>Proteins</subject><subject>Senescence</subject><subject>Stem cells</subject><subject>Umbilical cord</subject><subject>β-Galactosidase</subject><issn>1990-519X</issn><issn>1990-5203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1Uc1Kw0AQDqJgrT6AtwXP1dlNNmmOpf5UaFGsgrcw3c7alGRTd7fSvI2P6tZaPIinGb5fhomicw6XnMfJ1ZTnOUievwoACRyyg6izhXpSQHy43wN_HJ04twRIIeHQiT6vW4N1qRxrNJugt-WGTchjVTUr23gqDTpiA-XLj9K3DM2c3Wy8RUVVta7Q7j2PO7Fjw8Z4Mp6VhvkFbXFF7jv9iVZVqTAkEZuSIafIKNoyo3WNJtS6ACzaGis29VSzYehwp9GRxsrR2c_sRi-3N8_DUW_8cHc_HIx7SsQ866mYZnNMY9SgZ-kMlU6TVCcy66c0FyKXhAhCpomSidL5LJNaQ5r1Sc5B9HkWd6OLXW44-31NzhfLZm1NqCxEIkHEkPXjoOI7lbKNc5Z0sbJljbYtOBTbRxR_HhE8YudxQWveyP4m_2_6Amfsjcc</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Voronkina, I. V.</creator><creator>Smagina, L. V.</creator><creator>Bildyug, N. B.</creator><creator>Musorina, A. S.</creator><creator>Poljanskaya, G. G.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200901</creationdate><title>Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells</title><author>Voronkina, I. V. ; Smagina, L. V. ; Bildyug, N. B. ; Musorina, A. S. ; Poljanskaya, G. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2317-c3ebda63af0fb6bacf646f45786ed2295eaa02564c54cf9b75ff0678e5d028173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipose tissue</topic><topic>Aging</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Collagen (type I)</topic><topic>Comparative analysis</topic><topic>Coronary artery</topic><topic>Coronary artery disease</topic><topic>Enzymatic activity</topic><topic>Extracellular matrix</topic><topic>Eyelid</topic><topic>Fibronectin</topic><topic>Gelatinase A</topic><topic>Gelatinase B</topic><topic>Heart diseases</topic><topic>Heart surgery</topic><topic>Interstitial collagenase</topic><topic>Life Sciences</topic><topic>Matrix metalloproteinase</topic><topic>Mesenchymal stem cells</topic><topic>Metalloproteinase</topic><topic>Proteins</topic><topic>Senescence</topic><topic>Stem cells</topic><topic>Umbilical cord</topic><topic>β-Galactosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Voronkina, I. V.</creatorcontrib><creatorcontrib>Smagina, L. V.</creatorcontrib><creatorcontrib>Bildyug, N. B.</creatorcontrib><creatorcontrib>Musorina, A. S.</creatorcontrib><creatorcontrib>Poljanskaya, G. G.</creatorcontrib><collection>CrossRef</collection><jtitle>Cell and tissue biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Voronkina, I. V.</au><au>Smagina, L. V.</au><au>Bildyug, N. B.</au><au>Musorina, A. S.</au><au>Poljanskaya, G. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells</atitle><jtitle>Cell and tissue biology</jtitle><stitle>Cell Tiss. Biol</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>14</volume><issue>5</issue><spage>349</spage><epage>357</epage><pages>349-357</pages><issn>1990-519X</issn><eissn>1990-5203</eissn><abstract>A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matrix metalloproteinases (MMPs). In this work, we compared the dynamics of MMP activity and the level of ECM proteins during replicative senescence of three lines of human MSCs obtained from Wharton’s jelly of human umbilical cord (MSCWJ-1 line), eyelid skin (DF-2 line), and human epicardial adipose tissue isolated during coronary artery bypass grafting (ADH-MSC line). The fractions of cells with β-galactosidase enzyme activity (marker of replicative senescence) and the content of ECM proteins (fibronectin and type I collagen), as well as the activity of MMP-1, MMP-2, and MMP-9, were analyzed during long-term cultivation. It was found that three lines differ in the content of fibronectin, type 1 collagen, and MMP activity during the replicative senescence. Cells of the ADH-MSC line are mostly different from the other two lines in terms of aging rate, ECM protein content, and MMP activity. The reason for this discrepancy is that the cells are obtained from the tissue of a patient having heart disease.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1990519X20050107</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1990-519X
ispartof Cell and tissue biology, 2020-09, Vol.14 (5), p.349-357
issn 1990-519X
1990-5203
language eng
recordid cdi_proquest_journals_2450230783
source SpringerLink Journals - AutoHoldings
subjects Adipose tissue
Aging
Biomedical and Life Sciences
Cell Biology
Collagen (type I)
Comparative analysis
Coronary artery
Coronary artery disease
Enzymatic activity
Extracellular matrix
Eyelid
Fibronectin
Gelatinase A
Gelatinase B
Heart diseases
Heart surgery
Interstitial collagenase
Life Sciences
Matrix metalloproteinase
Mesenchymal stem cells
Metalloproteinase
Proteins
Senescence
Stem cells
Umbilical cord
β-Galactosidase
title Dynamics of Matrix Metalloproteinase Activity and Extracellular Matrix Proteins Content in the Process of Replicative Senescence of Human Mesenchymal Stem Cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A39%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dynamics%20of%20Matrix%20Metalloproteinase%20Activity%20and%20Extracellular%20Matrix%20Proteins%20Content%20in%20the%20Process%20of%20Replicative%20Senescence%20of%20Human%20Mesenchymal%20Stem%20Cells&rft.jtitle=Cell%20and%20tissue%20biology&rft.au=Voronkina,%20I.%20V.&rft.date=2020-09-01&rft.volume=14&rft.issue=5&rft.spage=349&rft.epage=357&rft.pages=349-357&rft.issn=1990-519X&rft.eissn=1990-5203&rft_id=info:doi/10.1134/S1990519X20050107&rft_dat=%3Cproquest_cross%3E2450230783%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2450230783&rft_id=info:pmid/&rfr_iscdi=true