Flower-like metal-organic framework microsphere as a novel enhanced ECL luminophore to construct the coreactant-free biosensor for ultrasensitive detection of breast cancer 1 gene

•High-efficiency ZnMOF(Ru) was obtained via a simple solvothermal method.•The unique ZnMOF(Ru) increased the mass loading and utilization efficiency of Ru(dcbpy)32+ effectively.•ZnMOF(Ru)-based coreactant-free ECL biosensor was fabricated for ultrasensitive detection of BRCA1. Nowadays, serious rest...

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Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2020-10, Vol.320, p.128395, Article 128395
Hauptverfasser: Wang, Hui-Min, Wang, Ai-Jun, Yuan, Pei-Xin, Feng, Jiu-Ju
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Wang, Ai-Jun
Yuan, Pei-Xin
Feng, Jiu-Ju
description •High-efficiency ZnMOF(Ru) was obtained via a simple solvothermal method.•The unique ZnMOF(Ru) increased the mass loading and utilization efficiency of Ru(dcbpy)32+ effectively.•ZnMOF(Ru)-based coreactant-free ECL biosensor was fabricated for ultrasensitive detection of BRCA1. Nowadays, serious restriction by aggregation quenching and proton attack in aqueous media, electrochemiluminescence (ECL) signals of emitter hardly match the requirement of ultrasensitive biomedical detection in practice. In this work, a novel flower-like metal-organic framework microsphere was formed by chelating Zn ion with carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) in the presence of cetyltrimethylammonium bromide (CTAB), defined as ZnMOF(Ru) for simplicity. For that, the obtained ZnMOF(Ru) as a high-performance ECL emitter displayed 53-fold amplification in aqueous solution without any coreactant alternative to that of Ru(dcbpy)32+, coupled with the dramatically enhanced fluorescence quantum yield and stability of the intermediates. Thus, a “sandwich”-like ECL biosensor was developed for detecting breast cancer 1 gene (BRCA1), showing the wide linear range (1.0 fM–0.1 nM) and ultralow limit of detection (LOD) down to 0.71 fM. Hence, this work provides a universal and robust pattern to develop advanced ECL luminophores and exploit their potential applications in bioanalysis.
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Nowadays, serious restriction by aggregation quenching and proton attack in aqueous media, electrochemiluminescence (ECL) signals of emitter hardly match the requirement of ultrasensitive biomedical detection in practice. In this work, a novel flower-like metal-organic framework microsphere was formed by chelating Zn ion with carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) in the presence of cetyltrimethylammonium bromide (CTAB), defined as ZnMOF(Ru) for simplicity. For that, the obtained ZnMOF(Ru) as a high-performance ECL emitter displayed 53-fold amplification in aqueous solution without any coreactant alternative to that of Ru(dcbpy)32+, coupled with the dramatically enhanced fluorescence quantum yield and stability of the intermediates. Thus, a “sandwich”-like ECL biosensor was developed for detecting breast cancer 1 gene (BRCA1), showing the wide linear range (1.0 fM–0.1 nM) and ultralow limit of detection (LOD) down to 0.71 fM. Hence, this work provides a universal and robust pattern to develop advanced ECL luminophores and exploit their potential applications in bioanalysis.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2020.128395</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Aqueous solutions ; Biosensors ; Breast cancer ; Breast cancer 1 gene ; Cetyltrimethylammonium bromide ; Chelation ; Dicarboxylic acids ; Electrochemiluminescence ; Emitters ; Fluorescence ; Luminophore ; Metal-organic frameworks ; Ruthenium ; Ruthenium compounds ; Zinc</subject><ispartof>Sensors and actuators. B, Chemical, 2020-10, Vol.320, p.128395, Article 128395</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. 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B, Chemical</title><description>•High-efficiency ZnMOF(Ru) was obtained via a simple solvothermal method.•The unique ZnMOF(Ru) increased the mass loading and utilization efficiency of Ru(dcbpy)32+ effectively.•ZnMOF(Ru)-based coreactant-free ECL biosensor was fabricated for ultrasensitive detection of BRCA1. Nowadays, serious restriction by aggregation quenching and proton attack in aqueous media, electrochemiluminescence (ECL) signals of emitter hardly match the requirement of ultrasensitive biomedical detection in practice. In this work, a novel flower-like metal-organic framework microsphere was formed by chelating Zn ion with carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) in the presence of cetyltrimethylammonium bromide (CTAB), defined as ZnMOF(Ru) for simplicity. 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B, Chemical</jtitle><date>2020-10-01</date><risdate>2020</risdate><volume>320</volume><spage>128395</spage><pages>128395-</pages><artnum>128395</artnum><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>•High-efficiency ZnMOF(Ru) was obtained via a simple solvothermal method.•The unique ZnMOF(Ru) increased the mass loading and utilization efficiency of Ru(dcbpy)32+ effectively.•ZnMOF(Ru)-based coreactant-free ECL biosensor was fabricated for ultrasensitive detection of BRCA1. Nowadays, serious restriction by aggregation quenching and proton attack in aqueous media, electrochemiluminescence (ECL) signals of emitter hardly match the requirement of ultrasensitive biomedical detection in practice. In this work, a novel flower-like metal-organic framework microsphere was formed by chelating Zn ion with carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) in the presence of cetyltrimethylammonium bromide (CTAB), defined as ZnMOF(Ru) for simplicity. For that, the obtained ZnMOF(Ru) as a high-performance ECL emitter displayed 53-fold amplification in aqueous solution without any coreactant alternative to that of Ru(dcbpy)32+, coupled with the dramatically enhanced fluorescence quantum yield and stability of the intermediates. Thus, a “sandwich”-like ECL biosensor was developed for detecting breast cancer 1 gene (BRCA1), showing the wide linear range (1.0 fM–0.1 nM) and ultralow limit of detection (LOD) down to 0.71 fM. Hence, this work provides a universal and robust pattern to develop advanced ECL luminophores and exploit their potential applications in bioanalysis.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2020.128395</doi><orcidid>https://orcid.org/0000-0002-4585-323X</orcidid></addata></record>
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subjects Aqueous solutions
Biosensors
Breast cancer
Breast cancer 1 gene
Cetyltrimethylammonium bromide
Chelation
Dicarboxylic acids
Electrochemiluminescence
Emitters
Fluorescence
Luminophore
Metal-organic frameworks
Ruthenium
Ruthenium compounds
Zinc
title Flower-like metal-organic framework microsphere as a novel enhanced ECL luminophore to construct the coreactant-free biosensor for ultrasensitive detection of breast cancer 1 gene
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