Morphometric Cell Classification for Single‐Cell MALDI‐Mass Spectrometry Imaging
The large‐scale and label‐free molecular characterization of single cells in their natural tissue habitat remains a major challenge in molecular biology. We present a method that integrates morphometric image analysis to delineate and classify individual cells with their single‐cell‐specific molecul...
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| Veröffentlicht in: | Angewandte Chemie 2020-09, Vol.132 (40), p.17600-17603 |
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| container_title | Angewandte Chemie |
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| creator | Ščupáková, Klára Dewez, Frédéric Walch, Axel K. Heeren, Ron M. A. Balluff, Benjamin |
| description | The large‐scale and label‐free molecular characterization of single cells in their natural tissue habitat remains a major challenge in molecular biology. We present a method that integrates morphometric image analysis to delineate and classify individual cells with their single‐cell‐specific molecular profiles. This approach provides a new means to study spatial biological processes such as cancer field effects and the relationship between morphometric and molecular features.
Understanding how cells regulate their bioenergetic demands, adjust to the microenvironment, or respond to stimuli is the aim of molecular biology. Yet, methods able to describe the molecular content, histological identity, and spatial context of a single cell do not exist. The presented method integrates morphometric image analysis to delineate and classify individual cells with their cell‐specific molecular profiles obtained by MALDI‐MSI. |
| doi_str_mv | 10.1002/ange.202007315 |
| format | Article |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological activity Chemistry Image analysis Image classification Image processing imaging lipids Mass spectrometry Mass spectroscopy Molecular biology morphometry single-cell analysis |
| title | Morphometric Cell Classification for Single‐Cell MALDI‐Mass Spectrometry Imaging |
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