Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells
Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA in...
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| Veröffentlicht in: | Toxicology in vitro 2020-08, Vol.66, p.104871, Article 104871 |
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| creator | Castillo-Sanchez, Rocio Ramirez-Ricardo, Javier Martinez-Baeza, Elia Cortes-Reynosa, Pedro Candanedo-Gonzales, Fernando Gomez, Rocio Salazar, Eduardo Perez |
| description | Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA induces proliferation and migration in human breast cancer cells. The G protein-coupled estrogen receptor (GPER) is a G protein-coupled receptor coupled with Gs proteins that is activated by estrogen and estrogenic compounds and it is the receptor for BPA. However, the signal transduction pathways that mediate migration via BPA/GPER in triple negative breast cancer (TNBC) cells has not been studied in detail. Here, we demonstrate that BPA induces an increase of GPER expression and activation of FAK, Src and ERK2, and an increase of focal adhesion assembly via GPER in TNBC MDA-MB-231 cells. Moreover, BPA induces FAK and ERK2 activation, focal adhesion assembly and migration via epidermal growth factor receptor (EGFR) transactivation. Collectively our data showed that BPA via GPER and/or EGFR transactivation induces activation of signal transduction pathways that mediate migration in TNBC MDA-MB-231 cells.
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•BPA induces migration via EGFR transactivation.•BPA induces FAK/ERK2 activation via GPER/EGFR transactivation.•BPA induces focal adhesion assembly via GPER/EGFR transactivation. |
| doi_str_mv | 10.1016/j.tiv.2020.104871 |
| format | Article |
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•BPA induces migration via EGFR transactivation.•BPA induces FAK/ERK2 activation via GPER/EGFR transactivation.•BPA induces focal adhesion assembly via GPER/EGFR transactivation.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2020.104871</identifier><identifier>PMID: 32325111</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Activation ; Adhesion ; Assembly ; Benzhydryl Compounds - toxicity ; Beverage cans ; Bisphenol A ; Breast cancer ; Cell Line, Tumor ; Cell Movement - drug effects ; Cell proliferation ; Containers ; Epidermal growth factor ; Epidermal growth factor receptors ; Epoxy resins ; Estrogens ; Extracellular signal-regulated kinase ; Focal adhesion ; Focal adhesion kinase ; Focal Adhesions - drug effects ; GPER, FAK ; Growth factors ; Humans ; Migration ; Phenols - toxicity ; Plasticizers - toxicity ; Polycarbonate ; Polycarbonate resins ; Polymers ; Protein-Tyrosine Kinases - metabolism ; Proteins ; Receptors ; Receptors, Estrogen - genetics ; Receptors, Estrogen - metabolism ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Signal transduction ; Src ; Src protein ; Triple Negative Breast Neoplasms - metabolism ; Xenoestrogens</subject><ispartof>Toxicology in vitro, 2020-08, Vol.66, p.104871, Article 104871</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Aug 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-15a605807a4e2b787f4931ccf977cd14968af2519ff875d99c95bc9963c5c4b93</citedby><cites>FETCH-LOGICAL-c381t-15a605807a4e2b787f4931ccf977cd14968af2519ff875d99c95bc9963c5c4b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233320300011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32325111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castillo-Sanchez, Rocio</creatorcontrib><creatorcontrib>Ramirez-Ricardo, Javier</creatorcontrib><creatorcontrib>Martinez-Baeza, Elia</creatorcontrib><creatorcontrib>Cortes-Reynosa, Pedro</creatorcontrib><creatorcontrib>Candanedo-Gonzales, Fernando</creatorcontrib><creatorcontrib>Gomez, Rocio</creatorcontrib><creatorcontrib>Salazar, Eduardo Perez</creatorcontrib><title>Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA induces proliferation and migration in human breast cancer cells. The G protein-coupled estrogen receptor (GPER) is a G protein-coupled receptor coupled with Gs proteins that is activated by estrogen and estrogenic compounds and it is the receptor for BPA. However, the signal transduction pathways that mediate migration via BPA/GPER in triple negative breast cancer (TNBC) cells has not been studied in detail. Here, we demonstrate that BPA induces an increase of GPER expression and activation of FAK, Src and ERK2, and an increase of focal adhesion assembly via GPER in TNBC MDA-MB-231 cells. Moreover, BPA induces FAK and ERK2 activation, focal adhesion assembly and migration via epidermal growth factor receptor (EGFR) transactivation. Collectively our data showed that BPA via GPER and/or EGFR transactivation induces activation of signal transduction pathways that mediate migration in TNBC MDA-MB-231 cells.
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•BPA induces migration via EGFR transactivation.•BPA induces FAK/ERK2 activation via GPER/EGFR transactivation.•BPA induces focal adhesion assembly via GPER/EGFR transactivation.</description><subject>Activation</subject><subject>Adhesion</subject><subject>Assembly</subject><subject>Benzhydryl Compounds - toxicity</subject><subject>Beverage cans</subject><subject>Bisphenol A</subject><subject>Breast cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Containers</subject><subject>Epidermal growth factor</subject><subject>Epidermal growth factor receptors</subject><subject>Epoxy resins</subject><subject>Estrogens</subject><subject>Extracellular signal-regulated kinase</subject><subject>Focal adhesion</subject><subject>Focal adhesion kinase</subject><subject>Focal Adhesions - drug effects</subject><subject>GPER, FAK</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Migration</subject><subject>Phenols - toxicity</subject><subject>Plasticizers - toxicity</subject><subject>Polycarbonate</subject><subject>Polycarbonate resins</subject><subject>Polymers</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, Estrogen - genetics</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Signal transduction</subject><subject>Src</subject><subject>Src protein</subject><subject>Triple Negative Breast Neoplasms - metabolism</subject><subject>Xenoestrogens</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFPGzEQha0KBIHyA3qpLHHtBo-9u7bVU4AAFSAq2p4t76wtHG12U3sTCfHncQjtkdNoNG_ezPsI-QJsCgzqs8V0DJspZ3zbl0rCJzIBJXUhQMo9MmFKyYILIQ7JUUoLxlilODsgh4ILXgHAhLych7R6cv3Q0RkNfbtGl6gf0HbUtk8uhaFP1Kbklk33TG3fUov5qB3zgA6eXs1uv9FfEd9G88dbTjfB0uuf88fsRu8vZ8X9eX4BaBOdTSNF26OLFF3Xpc9k39suuZP3ekz-XM1_X9wUdw_XPy5mdwUKBWMBla3z40za0vFGKulLLQDRaymxhVLXyvocR3uvZNVqjbpqUOtaYIVlo8UxOd35ruLwd-3SaBbDOvb5pOFlmVGAUnVWwU6FcUgpOm9WMSxtfDbAzBa3WZic3Gxxmx3uvPP13XndLF37f-Mf3yz4vhO4nG8TXDQJg8sI2hAdjqYdwgf2rwf6jJ4</recordid><startdate>202008</startdate><enddate>202008</enddate><creator>Castillo-Sanchez, Rocio</creator><creator>Ramirez-Ricardo, Javier</creator><creator>Martinez-Baeza, Elia</creator><creator>Cortes-Reynosa, Pedro</creator><creator>Candanedo-Gonzales, Fernando</creator><creator>Gomez, Rocio</creator><creator>Salazar, Eduardo Perez</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>202008</creationdate><title>Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells</title><author>Castillo-Sanchez, Rocio ; Ramirez-Ricardo, Javier ; Martinez-Baeza, Elia ; Cortes-Reynosa, Pedro ; Candanedo-Gonzales, Fernando ; Gomez, Rocio ; Salazar, Eduardo Perez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-15a605807a4e2b787f4931ccf977cd14968af2519ff875d99c95bc9963c5c4b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Activation</topic><topic>Adhesion</topic><topic>Assembly</topic><topic>Benzhydryl Compounds - toxicity</topic><topic>Beverage cans</topic><topic>Bisphenol A</topic><topic>Breast cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Cell proliferation</topic><topic>Containers</topic><topic>Epidermal growth factor</topic><topic>Epidermal growth factor receptors</topic><topic>Epoxy resins</topic><topic>Estrogens</topic><topic>Extracellular signal-regulated kinase</topic><topic>Focal adhesion</topic><topic>Focal adhesion kinase</topic><topic>Focal Adhesions - drug effects</topic><topic>GPER, FAK</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Migration</topic><topic>Phenols - toxicity</topic><topic>Plasticizers - toxicity</topic><topic>Polycarbonate</topic><topic>Polycarbonate resins</topic><topic>Polymers</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Estrogen - genetics</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Signal transduction</topic><topic>Src</topic><topic>Src protein</topic><topic>Triple Negative Breast Neoplasms - metabolism</topic><topic>Xenoestrogens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castillo-Sanchez, Rocio</creatorcontrib><creatorcontrib>Ramirez-Ricardo, Javier</creatorcontrib><creatorcontrib>Martinez-Baeza, Elia</creatorcontrib><creatorcontrib>Cortes-Reynosa, Pedro</creatorcontrib><creatorcontrib>Candanedo-Gonzales, Fernando</creatorcontrib><creatorcontrib>Gomez, Rocio</creatorcontrib><creatorcontrib>Salazar, Eduardo Perez</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castillo-Sanchez, Rocio</au><au>Ramirez-Ricardo, Javier</au><au>Martinez-Baeza, Elia</au><au>Cortes-Reynosa, Pedro</au><au>Candanedo-Gonzales, Fernando</au><au>Gomez, Rocio</au><au>Salazar, Eduardo Perez</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2020-08</date><risdate>2020</risdate><volume>66</volume><spage>104871</spage><pages>104871-</pages><artnum>104871</artnum><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA induces proliferation and migration in human breast cancer cells. The G protein-coupled estrogen receptor (GPER) is a G protein-coupled receptor coupled with Gs proteins that is activated by estrogen and estrogenic compounds and it is the receptor for BPA. However, the signal transduction pathways that mediate migration via BPA/GPER in triple negative breast cancer (TNBC) cells has not been studied in detail. Here, we demonstrate that BPA induces an increase of GPER expression and activation of FAK, Src and ERK2, and an increase of focal adhesion assembly via GPER in TNBC MDA-MB-231 cells. Moreover, BPA induces FAK and ERK2 activation, focal adhesion assembly and migration via epidermal growth factor receptor (EGFR) transactivation. Collectively our data showed that BPA via GPER and/or EGFR transactivation induces activation of signal transduction pathways that mediate migration in TNBC MDA-MB-231 cells.
[Display omitted]
•BPA induces migration via EGFR transactivation.•BPA induces FAK/ERK2 activation via GPER/EGFR transactivation.•BPA induces focal adhesion assembly via GPER/EGFR transactivation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32325111</pmid><doi>10.1016/j.tiv.2020.104871</doi></addata></record> |
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| subjects | Activation Adhesion Assembly Benzhydryl Compounds - toxicity Beverage cans Bisphenol A Breast cancer Cell Line, Tumor Cell Movement - drug effects Cell proliferation Containers Epidermal growth factor Epidermal growth factor receptors Epoxy resins Estrogens Extracellular signal-regulated kinase Focal adhesion Focal adhesion kinase Focal Adhesions - drug effects GPER, FAK Growth factors Humans Migration Phenols - toxicity Plasticizers - toxicity Polycarbonate Polycarbonate resins Polymers Protein-Tyrosine Kinases - metabolism Proteins Receptors Receptors, Estrogen - genetics Receptors, Estrogen - metabolism Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Signal transduction Src Src protein Triple Negative Breast Neoplasms - metabolism Xenoestrogens |
| title | Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells |
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