Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM
Understanding the cell–substrate interactions has great significance in tissue regeneration therapies. However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibrobl...
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| Veröffentlicht in: | Applied nanoscience 2020-09, Vol.10 (9), p.3717-3724 |
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| container_title | Applied nanoscience |
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| creator | Liu, Yan Li, Li Yang, Yang Tian, Liguo Wu, Xiaomin Weng, Zhankun Guo, Xudong Lei, Zecheng Qu, Kaige Yan, Jin Wang, Zuobin |
| description | Understanding the cell–substrate interactions has great significance in tissue regeneration therapies. However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibroblasts cultured on hybrid nanoarrays using atomic force acoustic microscopy (AFAM). We fabricated hybrid silicon nanowires (SiNWs) and silicon nanoholes (SiNHs) on Si substrates to serve as biomimetic nanoarrays by employing laser interference lithography and the metal-assisted chemical etching (MacEtch) method. After the L929 cells were cultured on the nanoarrays, scanning electron microscopy (SEM) and AFAM were employed to investigate the surface and subsurface of L929 cells. It was suggested that fibroblasts could sense the morphology of the hybrid nanoarrays and membrane damage of fibroblasts on the hybrid nanoarrays were related to the nanostructures. This study can help guide the design of biointerfaces and provide a useful tool for the study of cell subsurfaces in diverse biological fields. |
| doi_str_mv | 10.1007/s13204-020-01470-3 |
| format | Article |
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However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibroblasts cultured on hybrid nanoarrays using atomic force acoustic microscopy (AFAM). We fabricated hybrid silicon nanowires (SiNWs) and silicon nanoholes (SiNHs) on Si substrates to serve as biomimetic nanoarrays by employing laser interference lithography and the metal-assisted chemical etching (MacEtch) method. After the L929 cells were cultured on the nanoarrays, scanning electron microscopy (SEM) and AFAM were employed to investigate the surface and subsurface of L929 cells. It was suggested that fibroblasts could sense the morphology of the hybrid nanoarrays and membrane damage of fibroblasts on the hybrid nanoarrays were related to the nanostructures. This study can help guide the design of biointerfaces and provide a useful tool for the study of cell subsurfaces in diverse biological fields.</description><identifier>ISSN: 2190-5509</identifier><identifier>EISSN: 2190-5517</identifier><identifier>DOI: 10.1007/s13204-020-01470-3</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Acoustic microscopy ; Atomic force microscopy ; Biomedical materials ; Biomimetics ; Chemical etching ; Chemistry and Materials Science ; Fibroblasts ; Materials Science ; Membrane Biology ; Microscopy ; Morphology ; Nanochemistry ; Nanotechnology ; Nanotechnology and Microengineering ; Nanowires ; Original Article ; Regeneration ; Silicon substrates ; Tissue engineering</subject><ispartof>Applied nanoscience, 2020-09, Vol.10 (9), p.3717-3724</ispartof><rights>King Abdulaziz City for Science and Technology 2020</rights><rights>King Abdulaziz City for Science and Technology 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-42daffa813ecfaa72616acf6e5eb61fbff54e0d17603ce75c38252d5b61b23dd3</citedby><cites>FETCH-LOGICAL-c356t-42daffa813ecfaa72616acf6e5eb61fbff54e0d17603ce75c38252d5b61b23dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13204-020-01470-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13204-020-01470-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Tian, Liguo</creatorcontrib><creatorcontrib>Wu, Xiaomin</creatorcontrib><creatorcontrib>Weng, Zhankun</creatorcontrib><creatorcontrib>Guo, Xudong</creatorcontrib><creatorcontrib>Lei, Zecheng</creatorcontrib><creatorcontrib>Qu, Kaige</creatorcontrib><creatorcontrib>Yan, Jin</creatorcontrib><creatorcontrib>Wang, Zuobin</creatorcontrib><title>Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM</title><title>Applied nanoscience</title><addtitle>Appl Nanosci</addtitle><description>Understanding the cell–substrate interactions has great significance in tissue regeneration therapies. However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibroblasts cultured on hybrid nanoarrays using atomic force acoustic microscopy (AFAM). We fabricated hybrid silicon nanowires (SiNWs) and silicon nanoholes (SiNHs) on Si substrates to serve as biomimetic nanoarrays by employing laser interference lithography and the metal-assisted chemical etching (MacEtch) method. After the L929 cells were cultured on the nanoarrays, scanning electron microscopy (SEM) and AFAM were employed to investigate the surface and subsurface of L929 cells. It was suggested that fibroblasts could sense the morphology of the hybrid nanoarrays and membrane damage of fibroblasts on the hybrid nanoarrays were related to the nanostructures. This study can help guide the design of biointerfaces and provide a useful tool for the study of cell subsurfaces in diverse biological fields.</description><subject>Acoustic microscopy</subject><subject>Atomic force microscopy</subject><subject>Biomedical materials</subject><subject>Biomimetics</subject><subject>Chemical etching</subject><subject>Chemistry and Materials Science</subject><subject>Fibroblasts</subject><subject>Materials Science</subject><subject>Membrane Biology</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Nanotechnology and Microengineering</subject><subject>Nanowires</subject><subject>Original Article</subject><subject>Regeneration</subject><subject>Silicon substrates</subject><subject>Tissue engineering</subject><issn>2190-5509</issn><issn>2190-5517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9UE1LAzEQDaJgqf0DnhY8r06-22MpVgsVL_YcstmkpqxJTdZK_72xK3pzLjOPee8N8xC6xnCLAeRdxpQAq4FADZhJqOkZGhE8g5pzLM9_Z5hdoknOOyjFmRSUj9BmFQ42936rex-2lXXOmj5X0VXZd97EUAUd4qdPttKhPYHX2BWQkj4WXqicb1JsOp2L7OB1NV_On67QhdNdtpOfPkab5f3L4rFePz-sFvN1bSgXfc1Iq53TU0ytcVpLIrDQxgnLbSOwa5zjzEKLpQBqrOSGTgknLS_LhtC2pWN0M_juU3z_KH-oXfxIoZxUhDGYUcqwLCwysEyKOSfr1D75N52OCoP6TlANCaqSoDolqGgR0UGUCzlsbfqz_kf1BUrcdBE</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Liu, Yan</creator><creator>Li, Li</creator><creator>Yang, Yang</creator><creator>Tian, Liguo</creator><creator>Wu, Xiaomin</creator><creator>Weng, Zhankun</creator><creator>Guo, Xudong</creator><creator>Lei, Zecheng</creator><creator>Qu, Kaige</creator><creator>Yan, Jin</creator><creator>Wang, Zuobin</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200901</creationdate><title>Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM</title><author>Liu, Yan ; Li, Li ; Yang, Yang ; Tian, Liguo ; Wu, Xiaomin ; Weng, Zhankun ; Guo, Xudong ; Lei, Zecheng ; Qu, Kaige ; Yan, Jin ; Wang, Zuobin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-42daffa813ecfaa72616acf6e5eb61fbff54e0d17603ce75c38252d5b61b23dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acoustic microscopy</topic><topic>Atomic force microscopy</topic><topic>Biomedical materials</topic><topic>Biomimetics</topic><topic>Chemical etching</topic><topic>Chemistry and Materials Science</topic><topic>Fibroblasts</topic><topic>Materials Science</topic><topic>Membrane Biology</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Nanotechnology and Microengineering</topic><topic>Nanowires</topic><topic>Original Article</topic><topic>Regeneration</topic><topic>Silicon substrates</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Tian, Liguo</creatorcontrib><creatorcontrib>Wu, Xiaomin</creatorcontrib><creatorcontrib>Weng, Zhankun</creatorcontrib><creatorcontrib>Guo, Xudong</creatorcontrib><creatorcontrib>Lei, Zecheng</creatorcontrib><creatorcontrib>Qu, Kaige</creatorcontrib><creatorcontrib>Yan, Jin</creatorcontrib><creatorcontrib>Wang, Zuobin</creatorcontrib><collection>CrossRef</collection><jtitle>Applied nanoscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yan</au><au>Li, Li</au><au>Yang, Yang</au><au>Tian, Liguo</au><au>Wu, Xiaomin</au><au>Weng, Zhankun</au><au>Guo, Xudong</au><au>Lei, Zecheng</au><au>Qu, Kaige</au><au>Yan, Jin</au><au>Wang, Zuobin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM</atitle><jtitle>Applied nanoscience</jtitle><stitle>Appl Nanosci</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>10</volume><issue>9</issue><spage>3717</spage><epage>3724</epage><pages>3717-3724</pages><issn>2190-5509</issn><eissn>2190-5517</eissn><abstract>Understanding the cell–substrate interactions has great significance in tissue regeneration therapies. However, the cell–substrate interactions are not well understood because the interface of cell–substrate is typically buried beneath the cells. This research investigated the subsurfaces of fibroblasts cultured on hybrid nanoarrays using atomic force acoustic microscopy (AFAM). We fabricated hybrid silicon nanowires (SiNWs) and silicon nanoholes (SiNHs) on Si substrates to serve as biomimetic nanoarrays by employing laser interference lithography and the metal-assisted chemical etching (MacEtch) method. After the L929 cells were cultured on the nanoarrays, scanning electron microscopy (SEM) and AFAM were employed to investigate the surface and subsurface of L929 cells. It was suggested that fibroblasts could sense the morphology of the hybrid nanoarrays and membrane damage of fibroblasts on the hybrid nanoarrays were related to the nanostructures. This study can help guide the design of biointerfaces and provide a useful tool for the study of cell subsurfaces in diverse biological fields.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s13204-020-01470-3</doi><tpages>8</tpages></addata></record> |
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| subjects | Acoustic microscopy Atomic force microscopy Biomedical materials Biomimetics Chemical etching Chemistry and Materials Science Fibroblasts Materials Science Membrane Biology Microscopy Morphology Nanochemistry Nanotechnology Nanotechnology and Microengineering Nanowires Original Article Regeneration Silicon substrates Tissue engineering |
| title | Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM |
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