Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina
Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, fie...
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| Veröffentlicht in: | Research in veterinary science 2020-10, Vol.132, p.332-337 |
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| creator | Torrents, Jorgelina Sarli, Macarena Rossner, Maria V. Toffaletti, José R. Morel, Nicolás Martínez, Norberto C. Webster, Anelise Mangold, Atilio J. Guglielmone, Alberto A. Nava, Santiago |
| description | Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, field trials to control natural infestations of R. microplus on cattle with a commercial formulation of ivermectin 3.15% were carried and jointly analyzed with the in vitro assays. The phenotypic response of the populations analyzed was not uniform. Five of them were classified as susceptible, four populations as resistant, and one in the category “incipient resistance”. Regarding the field trials, the therapeutic efficacy in a population classified with LIT as susceptible achieved values higher than 94% two weeks after treatment, and no reproductively viable females were observed after the second day post-treatment. Conversely, the values of efficacy percentage in a population (named as “San Martín”) classified with LIT in the category “incipient resistance” never exceeded the 70.8%, and engorged females were collected in practically all counts. The population “San Martín” was classified in the category “incipient resistant” with LIT analysis, but the field trial unambiguously shows that this tick population is resistant. The comparison of the results obtained with LIT in vitro assays and through field trials shows that biased estimations of resistance levels may occur when resistance ratios (RR) values are ≤2, and additional field efficacy trials could be needed to know with precision the status of the tick populations evaluated.
•Resistance to ivermectin in R. microplus populations from Argentina was diagnosed.•In vitro larval immersion test and field trials were performed and jointly analyzed.•Different levels of resistance of R. microplus to ivermectin were detected in this work. |
| doi_str_mv | 10.1016/j.rvsc.2020.07.012 |
| format | Article |
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•Resistance to ivermectin in R. microplus populations from Argentina was diagnosed.•In vitro larval immersion test and field trials were performed and jointly analyzed.•Different levels of resistance of R. microplus to ivermectin were detected in this work.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2020.07.012</identifier><identifier>PMID: 32738729</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Arachnids ; Argentina ; Bioassays ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - parasitology ; Cattle tick ; Control ; Female ; Females ; Humidity ; In vitro methods and tests ; Insecticide Resistance ; Insecticides - pharmacology ; Ivermectin ; Ivermectin - pharmacology ; Larva ; Population ; Populations ; Resistance ; Rhipicephalus - drug effects ; Software ; Tick Infestations - drug therapy ; Tick Infestations - veterinary ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2020-10, Vol.132, p.332-337</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>2020. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8b7a62ee052c19e4ddf2101185ab6fa7f7e7da6c44948ddd129b2ad872a1e40e3</citedby><cites>FETCH-LOGICAL-c384t-8b7a62ee052c19e4ddf2101185ab6fa7f7e7da6c44948ddd129b2ad872a1e40e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0034528820309255$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32738729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torrents, Jorgelina</creatorcontrib><creatorcontrib>Sarli, Macarena</creatorcontrib><creatorcontrib>Rossner, Maria V.</creatorcontrib><creatorcontrib>Toffaletti, José R.</creatorcontrib><creatorcontrib>Morel, Nicolás</creatorcontrib><creatorcontrib>Martínez, Norberto C.</creatorcontrib><creatorcontrib>Webster, Anelise</creatorcontrib><creatorcontrib>Mangold, Atilio J.</creatorcontrib><creatorcontrib>Guglielmone, Alberto A.</creatorcontrib><creatorcontrib>Nava, Santiago</creatorcontrib><title>Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, field trials to control natural infestations of R. microplus on cattle with a commercial formulation of ivermectin 3.15% were carried and jointly analyzed with the in vitro assays. The phenotypic response of the populations analyzed was not uniform. Five of them were classified as susceptible, four populations as resistant, and one in the category “incipient resistance”. Regarding the field trials, the therapeutic efficacy in a population classified with LIT as susceptible achieved values higher than 94% two weeks after treatment, and no reproductively viable females were observed after the second day post-treatment. Conversely, the values of efficacy percentage in a population (named as “San Martín”) classified with LIT in the category “incipient resistance” never exceeded the 70.8%, and engorged females were collected in practically all counts. The population “San Martín” was classified in the category “incipient resistant” with LIT analysis, but the field trial unambiguously shows that this tick population is resistant. The comparison of the results obtained with LIT in vitro assays and through field trials shows that biased estimations of resistance levels may occur when resistance ratios (RR) values are ≤2, and additional field efficacy trials could be needed to know with precision the status of the tick populations evaluated.
•Resistance to ivermectin in R. microplus populations from Argentina was diagnosed.•In vitro larval immersion test and field trials were performed and jointly analyzed.•Different levels of resistance of R. microplus to ivermectin were detected in this work.</description><subject>Animals</subject><subject>Arachnids</subject><subject>Argentina</subject><subject>Bioassays</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle tick</subject><subject>Control</subject><subject>Female</subject><subject>Females</subject><subject>Humidity</subject><subject>In vitro methods and tests</subject><subject>Insecticide Resistance</subject><subject>Insecticides - pharmacology</subject><subject>Ivermectin</subject><subject>Ivermectin - pharmacology</subject><subject>Larva</subject><subject>Population</subject><subject>Populations</subject><subject>Resistance</subject><subject>Rhipicephalus - drug effects</subject><subject>Software</subject><subject>Tick Infestations - drug therapy</subject><subject>Tick Infestations - veterinary</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMobk6_gA8S8EUfWpM0bVrwZQ7_wUAY-iaENLl1mdtak2zgtzdl00fJhVwu557k_BA6pySlhBY3i9RtvU4ZYSQlIiWUHaAhzTOWsKKgh2hISMaTnJXlAJ14vyCEcErFMRpkTGSlYNUQvc_AWx_UWgNuGxzmgLUKYQk4WP2JZ3PbWQ3dXC03Hl_dtW03t7G9xiurXdv109BiuwW3Ah3sGscauw9Yx16doqNGLT2c7e8Renu4f508JdOXx-fJeJrorOQhKWuhCgZAcqZpBdyYhsWAtMxVXTRKNAKEUYXmvOKlMYayqmbKxACKAieQjdDlzrdz7dcGfJCLduPW8UnJeFaxePI8qthOFT_uvYNGds6ulPuWlMgeqFzIHqjsgUoiZAQaly721pt6BeZv5ZdgFNzuBBADbi046bWFiNNYF4lI09r__H8Agw2H4g</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Torrents, Jorgelina</creator><creator>Sarli, Macarena</creator><creator>Rossner, Maria V.</creator><creator>Toffaletti, José R.</creator><creator>Morel, Nicolás</creator><creator>Martínez, Norberto C.</creator><creator>Webster, Anelise</creator><creator>Mangold, Atilio J.</creator><creator>Guglielmone, Alberto A.</creator><creator>Nava, Santiago</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>202010</creationdate><title>Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina</title><author>Torrents, Jorgelina ; 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The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, field trials to control natural infestations of R. microplus on cattle with a commercial formulation of ivermectin 3.15% were carried and jointly analyzed with the in vitro assays. The phenotypic response of the populations analyzed was not uniform. Five of them were classified as susceptible, four populations as resistant, and one in the category “incipient resistance”. Regarding the field trials, the therapeutic efficacy in a population classified with LIT as susceptible achieved values higher than 94% two weeks after treatment, and no reproductively viable females were observed after the second day post-treatment. Conversely, the values of efficacy percentage in a population (named as “San Martín”) classified with LIT in the category “incipient resistance” never exceeded the 70.8%, and engorged females were collected in practically all counts. The population “San Martín” was classified in the category “incipient resistant” with LIT analysis, but the field trial unambiguously shows that this tick population is resistant. The comparison of the results obtained with LIT in vitro assays and through field trials shows that biased estimations of resistance levels may occur when resistance ratios (RR) values are ≤2, and additional field efficacy trials could be needed to know with precision the status of the tick populations evaluated.
•Resistance to ivermectin in R. microplus populations from Argentina was diagnosed.•In vitro larval immersion test and field trials were performed and jointly analyzed.•Different levels of resistance of R. microplus to ivermectin were detected in this work.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32738729</pmid><doi>10.1016/j.rvsc.2020.07.012</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Arachnids Argentina Bioassays Cattle Cattle Diseases - epidemiology Cattle Diseases - parasitology Cattle tick Control Female Females Humidity In vitro methods and tests Insecticide Resistance Insecticides - pharmacology Ivermectin Ivermectin - pharmacology Larva Population Populations Resistance Rhipicephalus - drug effects Software Tick Infestations - drug therapy Tick Infestations - veterinary Veterinary medicine |
| title | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
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