Process development for quantitation and vaccine efficacy assessment of recombinant hemagglutinin-neuraminidase

[Display omitted] •Extraction and two-stage purification strategy was developed to obtain rHN (∼95%).•Quantitative ELISA developed using purified rHN reference standards was validated.•Recovery of rHN at different stages of purification was monitored.•Optimal HI antibody titers and reduction in viru...

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Veröffentlicht in:	Process biochemistry (1991) 2020-03, Vol.90, p.204-214
Hauptverfasser:	Lye, Ping Ying, Noor, Suriani Mohd, Shohaimi, Syamsiah Aini, Junoh, Niny Fariza, Tan, Soo Choon, Iwamoto, Shinichi, Kotani, Eiji, Norazmi, Mohd Nor, Nagamine, Toshihiro, Mori, Hajime, Liew, Mervyn W.O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical methods Antibodies Antibody response Cell culture Column chromatography ELISA Exo-a-sialidase Hemagglutination inhibition Hemagglutinin-neuraminidase Hemagglutinins Herd immunity Histidine Hydroxyapatite Insects Ion exchange Lysates Newcastle disease Newcastle disease virus Poultry Purification Quantitation Robustness Subunit vaccine Vaccine efficacy Vaccine quantitation Vaccines Viruses
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