Enzyme Instructed Self‐assembly of Naphthalimide‐dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation
Understanding the structure‐morphology relationships of self‐assembled nanostructures is crucial for developing materials with the desired chemical and biological functions. Here, phosphate‐based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme‐instructed sel...
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description | Understanding the structure‐morphology relationships of self‐assembled nanostructures is crucial for developing materials with the desired chemical and biological functions. Here, phosphate‐based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme‐instructed self‐assembly process. Self‐assembly of simple amino acid derivative NI‐Yp resulted in non‐specific amorphous aggregates in the presence of alkaline phosphatase enzyme. On the other hand, NI‐FYp dipeptide forms spherical nanoparticles under aqueous conditions which slowly transformed into partially unzipped nanotubular structures during the enzymatic catalytic process through multiple stages which subsequently resulted in hydrogelation. The self‐assembly is driven by the formation of β‐sheet type structures stabilized by offset aromatic stacking of NI core and hydrogen bonding interactions which is confirmed with PXRD, Congo‐red staining and molecular mechanical calculations. We propose a mechanism for the self‐assembly process based on TEM and spectroscopic data. The nanotubular structures of NI‐FYp precursor exhibited higher cytotoxicity to human breast cancer cells and human cervical cancer cells when compared to the nanofiber structures of the similar Fmoc‐derivative. Overall this study provides a new understanding of the supramolecular self‐assembly of small‐molecular‐weight hydrogelators.
The first example of a phosphate‐based naphthalimide (NI)/dipeptide conjugate is reported that forms supramolecular hydrogels under the enzyme‐instructed self‐assembly process. |
doi_str_mv | 10.1002/asia.202000575 |
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The first example of a phosphate‐based naphthalimide (NI)/dipeptide conjugate is reported that forms supramolecular hydrogels under the enzyme‐instructed self‐assembly process.</description><identifier>ISSN: 1861-4728</identifier><identifier>EISSN: 1861-471X</identifier><identifier>DOI: 10.1002/asia.202000575</identifier><language>eng</language><publisher>Weinheim: Wiley Subscription Services, Inc</publisher><subject>Alkaline phosphatase ; Assembly ; Breast cancer ; Cervical cancer ; Chemistry ; Enzyme catalysis ; Enzymes ; Hydrogel ; Hydrogen bonding ; Mathematical analysis ; Morphology ; Nanofibers ; Nanoparticles ; Nanospheres ; Naphthalimide ; Peptide ; Supramolecular chemistry ; Toxicity</subject><ispartof>Chemistry, an Asian journal, 2020-09, Vol.15 (17), p.2696-2705</ispartof><rights>2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2020 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3875-ca6d03232d565679c275c6ada009efabc56743ea040a2a53c1a4a2c2112845913</citedby><cites>FETCH-LOGICAL-c3875-ca6d03232d565679c275c6ada009efabc56743ea040a2a53c1a4a2c2112845913</cites><orcidid>0000-0002-3596-3224</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fasia.202000575$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fasia.202000575$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Chakravarthy, Rajan Deepan</creatorcontrib><creatorcontrib>Mohammed, Mohiuddin</creatorcontrib><creatorcontrib>Lin, Hsin‐Chieh</creatorcontrib><title>Enzyme Instructed Self‐assembly of Naphthalimide‐dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation</title><title>Chemistry, an Asian journal</title><description>Understanding the structure‐morphology relationships of self‐assembled nanostructures is crucial for developing materials with the desired chemical and biological functions. Here, phosphate‐based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme‐instructed self‐assembly process. Self‐assembly of simple amino acid derivative NI‐Yp resulted in non‐specific amorphous aggregates in the presence of alkaline phosphatase enzyme. On the other hand, NI‐FYp dipeptide forms spherical nanoparticles under aqueous conditions which slowly transformed into partially unzipped nanotubular structures during the enzymatic catalytic process through multiple stages which subsequently resulted in hydrogelation. The self‐assembly is driven by the formation of β‐sheet type structures stabilized by offset aromatic stacking of NI core and hydrogen bonding interactions which is confirmed with PXRD, Congo‐red staining and molecular mechanical calculations. We propose a mechanism for the self‐assembly process based on TEM and spectroscopic data. The nanotubular structures of NI‐FYp precursor exhibited higher cytotoxicity to human breast cancer cells and human cervical cancer cells when compared to the nanofiber structures of the similar Fmoc‐derivative. Overall this study provides a new understanding of the supramolecular self‐assembly of small‐molecular‐weight hydrogelators.
The first example of a phosphate‐based naphthalimide (NI)/dipeptide conjugate is reported that forms supramolecular hydrogels under the enzyme‐instructed self‐assembly process.</description><subject>Alkaline phosphatase</subject><subject>Assembly</subject><subject>Breast cancer</subject><subject>Cervical cancer</subject><subject>Chemistry</subject><subject>Enzyme catalysis</subject><subject>Enzymes</subject><subject>Hydrogel</subject><subject>Hydrogen bonding</subject><subject>Mathematical analysis</subject><subject>Morphology</subject><subject>Nanofibers</subject><subject>Nanoparticles</subject><subject>Nanospheres</subject><subject>Naphthalimide</subject><subject>Peptide</subject><subject>Supramolecular chemistry</subject><subject>Toxicity</subject><issn>1861-4728</issn><issn>1861-471X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFUctKw0AUDaJgrW5dD7hOnUee7kpRWyi6aAV34XZyY1OSTJyZIHHlJ_gH_ptf4rSVunR1X-dx4XjeJaMjRim_BlPCiFNOKQ3j8MgbsCRifhCz5-NDz5NT78yYjYNwmiYD7-u2ee9rJLPGWN1JizlZYFV8f3yCMVivqp6ogjxAu7ZrqMq6zNHd8rLF1rr-hixa1VhoUHWGLDU0plC6BluqhhRa1Y7aKNOuUSOxajfZbtVVoMliZ9hpNMRpW_dC3kk3TPtcqxesdiLn3kkBlcGL3zr0nu5ul5OpP3-8n03Gc1-KJA59CVFOBRc8D6MwilPJ41BGkAOlKRawkm4ZCAQaUOAQCskgAC45YzwJwpSJoXe11221eu3Q2GyjOt04y4wHIkkiQYVwqNEeJbUyRmORtbqsQfcZo9k2hGwbQnYIwRHSPeGtrLD_B52NF7PxH_cHE_aRsA</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Chakravarthy, Rajan Deepan</creator><creator>Mohammed, Mohiuddin</creator><creator>Lin, Hsin‐Chieh</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-3596-3224</orcidid></search><sort><creationdate>20200901</creationdate><title>Enzyme Instructed Self‐assembly of Naphthalimide‐dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation</title><author>Chakravarthy, Rajan Deepan ; Mohammed, Mohiuddin ; Lin, Hsin‐Chieh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3875-ca6d03232d565679c275c6ada009efabc56743ea040a2a53c1a4a2c2112845913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaline phosphatase</topic><topic>Assembly</topic><topic>Breast cancer</topic><topic>Cervical cancer</topic><topic>Chemistry</topic><topic>Enzyme catalysis</topic><topic>Enzymes</topic><topic>Hydrogel</topic><topic>Hydrogen bonding</topic><topic>Mathematical analysis</topic><topic>Morphology</topic><topic>Nanofibers</topic><topic>Nanoparticles</topic><topic>Nanospheres</topic><topic>Naphthalimide</topic><topic>Peptide</topic><topic>Supramolecular chemistry</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chakravarthy, Rajan Deepan</creatorcontrib><creatorcontrib>Mohammed, Mohiuddin</creatorcontrib><creatorcontrib>Lin, Hsin‐Chieh</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Chemistry, an Asian journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chakravarthy, Rajan Deepan</au><au>Mohammed, Mohiuddin</au><au>Lin, Hsin‐Chieh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme Instructed Self‐assembly of Naphthalimide‐dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation</atitle><jtitle>Chemistry, an Asian journal</jtitle><date>2020-09-01</date><risdate>2020</risdate><volume>15</volume><issue>17</issue><spage>2696</spage><epage>2705</epage><pages>2696-2705</pages><issn>1861-4728</issn><eissn>1861-471X</eissn><abstract>Understanding the structure‐morphology relationships of self‐assembled nanostructures is crucial for developing materials with the desired chemical and biological functions. Here, phosphate‐based naphthalimide (NI) derivatives have been developed for the first time to study the enzyme‐instructed self‐assembly process. Self‐assembly of simple amino acid derivative NI‐Yp resulted in non‐specific amorphous aggregates in the presence of alkaline phosphatase enzyme. On the other hand, NI‐FYp dipeptide forms spherical nanoparticles under aqueous conditions which slowly transformed into partially unzipped nanotubular structures during the enzymatic catalytic process through multiple stages which subsequently resulted in hydrogelation. The self‐assembly is driven by the formation of β‐sheet type structures stabilized by offset aromatic stacking of NI core and hydrogen bonding interactions which is confirmed with PXRD, Congo‐red staining and molecular mechanical calculations. We propose a mechanism for the self‐assembly process based on TEM and spectroscopic data. The nanotubular structures of NI‐FYp precursor exhibited higher cytotoxicity to human breast cancer cells and human cervical cancer cells when compared to the nanofiber structures of the similar Fmoc‐derivative. Overall this study provides a new understanding of the supramolecular self‐assembly of small‐molecular‐weight hydrogelators.
The first example of a phosphate‐based naphthalimide (NI)/dipeptide conjugate is reported that forms supramolecular hydrogels under the enzyme‐instructed self‐assembly process.</abstract><cop>Weinheim</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/asia.202000575</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3596-3224</orcidid></addata></record> |
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subjects | Alkaline phosphatase Assembly Breast cancer Cervical cancer Chemistry Enzyme catalysis Enzymes Hydrogel Hydrogen bonding Mathematical analysis Morphology Nanofibers Nanoparticles Nanospheres Naphthalimide Peptide Supramolecular chemistry Toxicity |
title | Enzyme Instructed Self‐assembly of Naphthalimide‐dipeptide: Spontaneous Transformation from Nanosphere to Nanotubular Structures that Induces Hydrogelation |
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