Bacterial cellulose sponges obtained with green cross-linkers for tissue engineering
Three-dimensional (3D) porous structures with controlled pore size and interconnected pores, good mechanical properties and biocompatibility are of great interest for tissue engineering. In this work we propose a new strategy to obtain highly porous 3D structures with improved properties using bacte...
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| Veröffentlicht in: | Materials Science & Engineering C 2020-05, Vol.110, p.110740, Article 110740 |
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| creator | Frone, Adriana Nicoleta Panaitescu, Denis Mihaela Nicolae, Cristian Andi Gabor, Augusta Raluca Trusca, Roxana Casarica, Angela Stanescu, Paul Octavian Baciu, Dora Domnica Salageanu, Aurora |
| description | Three-dimensional (3D) porous structures with controlled pore size and interconnected pores, good mechanical properties and biocompatibility are of great interest for tissue engineering. In this work we propose a new strategy to obtain highly porous 3D structures with improved properties using bacterial cellulose (BC) and eco-friendly additives and processes. Glucose, vanillin and citric acid were used as non-toxic and cheap cross-linkers and γ-aminopropyltriethoxysilane was used to partially replace the surface OH groups of cellulose with amino groups. The efficiency of grafting and cross-linking reactions was confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The morphological investigation of BC sponges revealed a multi-hierarchical organization after functionalization and cross-linking. Micro-computed tomography analysis showed 80–90% open porosity in modified BC sponges. The thermal and mechanical properties of the sponges were influenced by the cross-linker type and concentration. The strength-to-weight ratio of BC sponges cross-linked with glucose and citric acid was 150% and 120% higher compared to that of unmodified BC sponge. In vitro assays revealed that the modified BC sponges are non-cytotoxic and do not trigger an inflammatory response in macrophages. This study provides a simple and green method to obtain highly porous cellulose sponges with hierarchical design, biocompatibility and good mechanical properties.
•Nanocellulose was obtained from bacterial cellulose (BC) membranes by defibrillation.•Green cross-linkers (vanillin, glucose, citric acid) were used to obtain BC sponges.•Improved mechanical properties obtained with citric acid and glucose-G crosslinkers•Crosslinked BC sponges are non-cytotoxic and have no inflammatory response except for G. |
| doi_str_mv | 10.1016/j.msec.2020.110740 |
| format | Article |
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•Nanocellulose was obtained from bacterial cellulose (BC) membranes by defibrillation.•Green cross-linkers (vanillin, glucose, citric acid) were used to obtain BC sponges.•Improved mechanical properties obtained with citric acid and glucose-G crosslinkers•Crosslinked BC sponges are non-cytotoxic and have no inflammatory response except for G.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2020.110740</identifier><identifier>PMID: 32204048</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Additives ; Amino groups ; Aminopropyltriethoxysilane ; Animals ; Bacteria - chemistry ; Bacterial cellulose ; Biocompatibility ; Cell Line ; Cellulose ; Cellulose - chemistry ; Citric acid ; Computed tomography ; Cross-Linking Reagents - chemistry ; Crosslinking ; Cytotoxicity ; Fourier analysis ; Fourier transforms ; Glucose ; Inflammation ; Inflammatory response ; Infrared spectroscopy ; Macrophages ; Materials science ; Materials Testing ; Mechanical properties ; Mice ; Nanofibrous sponge ; Photoelectron spectroscopy ; Photoelectrons ; Pore size ; Porosity ; Spectrum analysis ; Sponges ; Strength to weight ratio ; Surface functionalization ; Thermodynamic properties ; Tissue Engineering ; Tissue Scaffolds - chemistry ; Vanillin</subject><ispartof>Materials Science & Engineering C, 2020-05, Vol.110, p.110740, Article 110740</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV May 2020</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-cb4abdc6428317d0874687f6552d891dd31a1c64fc7dbb0d6bbc29d881f13b333</citedby><cites>FETCH-LOGICAL-c384t-cb4abdc6428317d0874687f6552d891dd31a1c64fc7dbb0d6bbc29d881f13b333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.msec.2020.110740$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32204048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frone, Adriana Nicoleta</creatorcontrib><creatorcontrib>Panaitescu, Denis Mihaela</creatorcontrib><creatorcontrib>Nicolae, Cristian Andi</creatorcontrib><creatorcontrib>Gabor, Augusta Raluca</creatorcontrib><creatorcontrib>Trusca, Roxana</creatorcontrib><creatorcontrib>Casarica, Angela</creatorcontrib><creatorcontrib>Stanescu, Paul Octavian</creatorcontrib><creatorcontrib>Baciu, Dora Domnica</creatorcontrib><creatorcontrib>Salageanu, Aurora</creatorcontrib><title>Bacterial cellulose sponges obtained with green cross-linkers for tissue engineering</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>Three-dimensional (3D) porous structures with controlled pore size and interconnected pores, good mechanical properties and biocompatibility are of great interest for tissue engineering. In this work we propose a new strategy to obtain highly porous 3D structures with improved properties using bacterial cellulose (BC) and eco-friendly additives and processes. Glucose, vanillin and citric acid were used as non-toxic and cheap cross-linkers and γ-aminopropyltriethoxysilane was used to partially replace the surface OH groups of cellulose with amino groups. The efficiency of grafting and cross-linking reactions was confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The morphological investigation of BC sponges revealed a multi-hierarchical organization after functionalization and cross-linking. Micro-computed tomography analysis showed 80–90% open porosity in modified BC sponges. The thermal and mechanical properties of the sponges were influenced by the cross-linker type and concentration. The strength-to-weight ratio of BC sponges cross-linked with glucose and citric acid was 150% and 120% higher compared to that of unmodified BC sponge. In vitro assays revealed that the modified BC sponges are non-cytotoxic and do not trigger an inflammatory response in macrophages. This study provides a simple and green method to obtain highly porous cellulose sponges with hierarchical design, biocompatibility and good mechanical properties.
•Nanocellulose was obtained from bacterial cellulose (BC) membranes by defibrillation.•Green cross-linkers (vanillin, glucose, citric acid) were used to obtain BC sponges.•Improved mechanical properties obtained with citric acid and glucose-G crosslinkers•Crosslinked BC sponges are non-cytotoxic and have no inflammatory response except for G.</description><subject>Additives</subject><subject>Amino groups</subject><subject>Aminopropyltriethoxysilane</subject><subject>Animals</subject><subject>Bacteria - chemistry</subject><subject>Bacterial cellulose</subject><subject>Biocompatibility</subject><subject>Cell Line</subject><subject>Cellulose</subject><subject>Cellulose - chemistry</subject><subject>Citric acid</subject><subject>Computed tomography</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Crosslinking</subject><subject>Cytotoxicity</subject><subject>Fourier analysis</subject><subject>Fourier transforms</subject><subject>Glucose</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Infrared spectroscopy</subject><subject>Macrophages</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Mechanical properties</subject><subject>Mice</subject><subject>Nanofibrous sponge</subject><subject>Photoelectron spectroscopy</subject><subject>Photoelectrons</subject><subject>Pore size</subject><subject>Porosity</subject><subject>Spectrum analysis</subject><subject>Sponges</subject><subject>Strength to weight ratio</subject><subject>Surface functionalization</subject><subject>Thermodynamic properties</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Vanillin</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwAyyQJdYpfjVxJDZQ8ZIqsSlrK7YnwSFNip2A-HtcUliyGmnmzp25B6FzSuaU0PSqnm8CmDkjLDYoyQQ5QFMqM54QmtNDNCU5k4nIOZ2gkxBqQlLJM3aMJpwxIoiQU7S-LUwP3hUNNtA0Q9MFwGHbtRUE3Om-cC1Y_On6V1x5gBYb34WQNK59Ax9w2XncuxAGwNBWURut2uoUHZVFE-BsX2fo5f5uvXxMVs8PT8ubVWK4FH1itCi0NalgktPMEpmJVGZlulgwK3NqLacFjePSZFZrYlOtDcutlLSkXHPOZ-hy9N367n2A0Ku6G3wbTyomuIwhWUw8Q2xU_bzuoVRb7zaF_1KUqB1IVasdSLUDqUaQcelibz3oDdi_lV9yUXA9CiAG_HDgVTAOWgPWeTC9sp37z_8bFD-EoQ</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Frone, Adriana Nicoleta</creator><creator>Panaitescu, Denis Mihaela</creator><creator>Nicolae, Cristian Andi</creator><creator>Gabor, Augusta Raluca</creator><creator>Trusca, Roxana</creator><creator>Casarica, Angela</creator><creator>Stanescu, Paul Octavian</creator><creator>Baciu, Dora Domnica</creator><creator>Salageanu, Aurora</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>202005</creationdate><title>Bacterial cellulose sponges obtained with green cross-linkers for tissue engineering</title><author>Frone, Adriana Nicoleta ; 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In this work we propose a new strategy to obtain highly porous 3D structures with improved properties using bacterial cellulose (BC) and eco-friendly additives and processes. Glucose, vanillin and citric acid were used as non-toxic and cheap cross-linkers and γ-aminopropyltriethoxysilane was used to partially replace the surface OH groups of cellulose with amino groups. The efficiency of grafting and cross-linking reactions was confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The morphological investigation of BC sponges revealed a multi-hierarchical organization after functionalization and cross-linking. Micro-computed tomography analysis showed 80–90% open porosity in modified BC sponges. The thermal and mechanical properties of the sponges were influenced by the cross-linker type and concentration. The strength-to-weight ratio of BC sponges cross-linked with glucose and citric acid was 150% and 120% higher compared to that of unmodified BC sponge. In vitro assays revealed that the modified BC sponges are non-cytotoxic and do not trigger an inflammatory response in macrophages. This study provides a simple and green method to obtain highly porous cellulose sponges with hierarchical design, biocompatibility and good mechanical properties.
•Nanocellulose was obtained from bacterial cellulose (BC) membranes by defibrillation.•Green cross-linkers (vanillin, glucose, citric acid) were used to obtain BC sponges.•Improved mechanical properties obtained with citric acid and glucose-G crosslinkers•Crosslinked BC sponges are non-cytotoxic and have no inflammatory response except for G.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>32204048</pmid><doi>10.1016/j.msec.2020.110740</doi></addata></record> |
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| subjects | Additives Amino groups Aminopropyltriethoxysilane Animals Bacteria - chemistry Bacterial cellulose Biocompatibility Cell Line Cellulose Cellulose - chemistry Citric acid Computed tomography Cross-Linking Reagents - chemistry Crosslinking Cytotoxicity Fourier analysis Fourier transforms Glucose Inflammation Inflammatory response Infrared spectroscopy Macrophages Materials science Materials Testing Mechanical properties Mice Nanofibrous sponge Photoelectron spectroscopy Photoelectrons Pore size Porosity Spectrum analysis Sponges Strength to weight ratio Surface functionalization Thermodynamic properties Tissue Engineering Tissue Scaffolds - chemistry Vanillin |
| title | Bacterial cellulose sponges obtained with green cross-linkers for tissue engineering |
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