In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?
Purpose Our purpose is to investigate the reasons why Lactobacillus iners is detected in abnormal vaginal microbial flora. Methods In this study, in vitro characteristics of four type strains ( L. crispatus , L. iners , L. gasseri , and L. jensenii) were examined by measuring the growth speed by OD...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2020-09, Vol.302 (3), p.671-677 |
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| creator | Yoshimura, Kazuaki Ogawa, Midori Saito, Mitsumasa |
| description | Purpose
Our purpose is to investigate the reasons why
Lactobacillus iners
is detected in abnormal vaginal microbial flora.
Methods
In this study, in vitro characteristics of four type strains (
L. crispatus
,
L. iners
,
L. gasseri
, and
L. jensenii)
were examined by measuring the growth speed by OD
660
, and acid resistance, with gram stain and Live/Dead stain.
Results
The growth speed was
L. gasseri
>
L. jensenii
>
L. crispatus
>
L. iners
. Bacterial counts of all
Lactobacilli
in MRS medium began to decrease at the middle of the log-phase of the growth curve. In addition,
L. iners
grew to 10
6
CFU/mL and the others grew to 10
8
CFU/mL.
L. iners
was mostly Gram-negative with very short rod, while the others were mostly Gram-positive rods.
L. iners
was completely killed in the pH 3 medium, however, the others grew (in pH 3 medium) in 1/100 order compared with those in the pH 6 medium.
Conclusion
L. iners
was not a typical gram-positive long rod
Lactobacilli
and presented weak acid-resistance. The reasons why
L. iners
is detected in abnormal vaginal microbial flora were presumed to be due to the unique morphologic and microbiologic characteristics. |
| doi_str_mv | 10.1007/s00404-020-05634-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2436978449</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2436978449</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-8ab2485e63a8f80f8fdf1f1a1086b6d7c15773ef95ece34a711c45f92e4dd44c3</originalsourceid><addsrcrecordid>eNp9kM1OwzAQhC0EoqXwAhyQJc4p69iJHXFAqOKnUiUucLYcx25dpUmx06K8PS5t4cbJa-18s7uD0DWBMQHgdwGAAUsghQSynLKkP0FDwmiaACfkFA2h2NWQ8wG6CGEJQFIh8nM0oGkGvBB8iLppg7eu8y3WC-WV7ox3oXM64NZi13RebdXcNarGs9hsS6VdXbt7_LXosQt4No4i4wOuTGciXMUvVmXT-lVEjujKad-WLla2br16uERnVtXBXB3eEfp4fnqfvCazt5fp5HGWaMqzLhGqTJnITE6VsAKssJUlligCIi_zimuScU6NLTKjDWUqHq1ZZovUsKpiTNMRut37rn37uTGhk8t24-NGQaaM5gUXjBVRle5VcckQvLFy7d1K-V4SkLug5T5oGYOWP0HLPkI3B-tNuTLVL3JMNgroXhBiq5kb_zf7H9tvqiGKuQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2436978449</pqid></control><display><type>article</type><title>In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Yoshimura, Kazuaki ; Ogawa, Midori ; Saito, Mitsumasa</creator><creatorcontrib>Yoshimura, Kazuaki ; Ogawa, Midori ; Saito, Mitsumasa</creatorcontrib><description>Purpose
Our purpose is to investigate the reasons why
Lactobacillus iners
is detected in abnormal vaginal microbial flora.
Methods
In this study, in vitro characteristics of four type strains (
L. crispatus
,
L. iners
,
L. gasseri
, and
L. jensenii)
were examined by measuring the growth speed by OD
660
, and acid resistance, with gram stain and Live/Dead stain.
Results
The growth speed was
L. gasseri
>
L. jensenii
>
L. crispatus
>
L. iners
. Bacterial counts of all
Lactobacilli
in MRS medium began to decrease at the middle of the log-phase of the growth curve. In addition,
L. iners
grew to 10
6
CFU/mL and the others grew to 10
8
CFU/mL.
L. iners
was mostly Gram-negative with very short rod, while the others were mostly Gram-positive rods.
L. iners
was completely killed in the pH 3 medium, however, the others grew (in pH 3 medium) in 1/100 order compared with those in the pH 6 medium.
Conclusion
L. iners
was not a typical gram-positive long rod
Lactobacilli
and presented weak acid-resistance. The reasons why
L. iners
is detected in abnormal vaginal microbial flora were presumed to be due to the unique morphologic and microbiologic characteristics.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-020-05634-y</identifier><identifier>PMID: 32507987</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacteria ; Cloning ; Drinking water ; Endocrinology ; Female ; General Gynecology ; Gynecology ; Human Genetics ; Humans ; Lactobacillus - pathogenicity ; Medicine ; Medicine & Public Health ; Methods ; Microscopy ; Obstetrics/Perinatology/Midwifery ; Principal components analysis ; Stains & staining ; Vagina ; Vagina - microbiology</subject><ispartof>Archives of gynecology and obstetrics, 2020-09, Vol.302 (3), p.671-677</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-8ab2485e63a8f80f8fdf1f1a1086b6d7c15773ef95ece34a711c45f92e4dd44c3</citedby><cites>FETCH-LOGICAL-c375t-8ab2485e63a8f80f8fdf1f1a1086b6d7c15773ef95ece34a711c45f92e4dd44c3</cites><orcidid>0000-0002-7463-1708</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-020-05634-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-020-05634-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32507987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshimura, Kazuaki</creatorcontrib><creatorcontrib>Ogawa, Midori</creatorcontrib><creatorcontrib>Saito, Mitsumasa</creatorcontrib><title>In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
Our purpose is to investigate the reasons why
Lactobacillus iners
is detected in abnormal vaginal microbial flora.
Methods
In this study, in vitro characteristics of four type strains (
L. crispatus
,
L. iners
,
L. gasseri
, and
L. jensenii)
were examined by measuring the growth speed by OD
660
, and acid resistance, with gram stain and Live/Dead stain.
Results
The growth speed was
L. gasseri
>
L. jensenii
>
L. crispatus
>
L. iners
. Bacterial counts of all
Lactobacilli
in MRS medium began to decrease at the middle of the log-phase of the growth curve. In addition,
L. iners
grew to 10
6
CFU/mL and the others grew to 10
8
CFU/mL.
L. iners
was mostly Gram-negative with very short rod, while the others were mostly Gram-positive rods.
L. iners
was completely killed in the pH 3 medium, however, the others grew (in pH 3 medium) in 1/100 order compared with those in the pH 6 medium.
Conclusion
L. iners
was not a typical gram-positive long rod
Lactobacilli
and presented weak acid-resistance. The reasons why
L. iners
is detected in abnormal vaginal microbial flora were presumed to be due to the unique morphologic and microbiologic characteristics.</description><subject>Bacteria</subject><subject>Cloning</subject><subject>Drinking water</subject><subject>Endocrinology</subject><subject>Female</subject><subject>General Gynecology</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Lactobacillus - pathogenicity</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Methods</subject><subject>Microscopy</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Principal components analysis</subject><subject>Stains & staining</subject><subject>Vagina</subject><subject>Vagina - microbiology</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kM1OwzAQhC0EoqXwAhyQJc4p69iJHXFAqOKnUiUucLYcx25dpUmx06K8PS5t4cbJa-18s7uD0DWBMQHgdwGAAUsghQSynLKkP0FDwmiaACfkFA2h2NWQ8wG6CGEJQFIh8nM0oGkGvBB8iLppg7eu8y3WC-WV7ox3oXM64NZi13RebdXcNarGs9hsS6VdXbt7_LXosQt4No4i4wOuTGciXMUvVmXT-lVEjujKad-WLla2br16uERnVtXBXB3eEfp4fnqfvCazt5fp5HGWaMqzLhGqTJnITE6VsAKssJUlligCIi_zimuScU6NLTKjDWUqHq1ZZovUsKpiTNMRut37rn37uTGhk8t24-NGQaaM5gUXjBVRle5VcckQvLFy7d1K-V4SkLug5T5oGYOWP0HLPkI3B-tNuTLVL3JMNgroXhBiq5kb_zf7H9tvqiGKuQ</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Yoshimura, Kazuaki</creator><creator>Ogawa, Midori</creator><creator>Saito, Mitsumasa</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0002-7463-1708</orcidid></search><sort><creationdate>20200901</creationdate><title>In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?</title><author>Yoshimura, Kazuaki ; Ogawa, Midori ; Saito, Mitsumasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-8ab2485e63a8f80f8fdf1f1a1086b6d7c15773ef95ece34a711c45f92e4dd44c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacteria</topic><topic>Cloning</topic><topic>Drinking water</topic><topic>Endocrinology</topic><topic>Female</topic><topic>General Gynecology</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Lactobacillus - pathogenicity</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Methods</topic><topic>Microscopy</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Principal components analysis</topic><topic>Stains & staining</topic><topic>Vagina</topic><topic>Vagina - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshimura, Kazuaki</creatorcontrib><creatorcontrib>Ogawa, Midori</creatorcontrib><creatorcontrib>Saito, Mitsumasa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshimura, Kazuaki</au><au>Ogawa, Midori</au><au>Saito, Mitsumasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>302</volume><issue>3</issue><spage>671</spage><epage>677</epage><pages>671-677</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
Our purpose is to investigate the reasons why
Lactobacillus iners
is detected in abnormal vaginal microbial flora.
Methods
In this study, in vitro characteristics of four type strains (
L. crispatus
,
L. iners
,
L. gasseri
, and
L. jensenii)
were examined by measuring the growth speed by OD
660
, and acid resistance, with gram stain and Live/Dead stain.
Results
The growth speed was
L. gasseri
>
L. jensenii
>
L. crispatus
>
L. iners
. Bacterial counts of all
Lactobacilli
in MRS medium began to decrease at the middle of the log-phase of the growth curve. In addition,
L. iners
grew to 10
6
CFU/mL and the others grew to 10
8
CFU/mL.
L. iners
was mostly Gram-negative with very short rod, while the others were mostly Gram-positive rods.
L. iners
was completely killed in the pH 3 medium, however, the others grew (in pH 3 medium) in 1/100 order compared with those in the pH 6 medium.
Conclusion
L. iners
was not a typical gram-positive long rod
Lactobacilli
and presented weak acid-resistance. The reasons why
L. iners
is detected in abnormal vaginal microbial flora were presumed to be due to the unique morphologic and microbiologic characteristics.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32507987</pmid><doi>10.1007/s00404-020-05634-y</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7463-1708</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0067 |
| ispartof | Archives of gynecology and obstetrics, 2020-09, Vol.302 (3), p.671-677 |
| issn | 0932-0067 1432-0711 |
| language | eng |
| recordid | cdi_proquest_journals_2436978449 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Bacteria Cloning Drinking water Endocrinology Female General Gynecology Gynecology Human Genetics Humans Lactobacillus - pathogenicity Medicine Medicine & Public Health Methods Microscopy Obstetrics/Perinatology/Midwifery Principal components analysis Stains & staining Vagina Vagina - microbiology |
| title | In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora? |
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