Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm
Infections due to Bombyx mori nucleopolyhedro virus ( Bm NPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes...
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| Veröffentlicht in: | International journal of tropical insect science 2020, Vol.40 (3), p.483-491 |
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| creator | Varada, Burdekar Pradeep, Appukuttan Nair R. Awasthi, Arvind K. Ponnuvel, Kangayam M. |
| description | Infections due to
Bombyx mori
nucleopolyhedro virus (
Bm
NPV) in commercially important silkworm,
Bombyx mori
L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in
B. mori
. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic
B. mori
. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of
gp-41
gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes,
ie-1, lef1
and
p74
enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. |
| doi_str_mv | 10.1007/s42690-020-00201-z |
| format | Article |
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Bombyx mori
nucleopolyhedro virus (
Bm
NPV) in commercially important silkworm,
Bombyx mori
L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in
B. mori
. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic
B. mori
. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of
gp-41
gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes,
ie-1, lef1
and
p74
enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes.</description><identifier>ISSN: 1742-7592</identifier><identifier>ISSN: 1742-7584</identifier><identifier>EISSN: 1742-7592</identifier><identifier>DOI: 10.1007/s42690-020-00201-z</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Antiviral activity ; Biomedical and Life Sciences ; Bombyx mori ; Copy number ; Double-stranded RNA ; Entomology ; Gene expression ; Genes ; Inbreeding ; Larvae ; Life Sciences ; Multiplication ; Original Research Article ; Ribonucleic acid ; RNA ; RNA-mediated interference ; Signs and symptoms ; Silk ; Silkworms ; Transgenes ; Viruses</subject><ispartof>International journal of tropical insect science, 2020, Vol.40 (3), p.483-491</ispartof><rights>African Association of Insect Scientists 2020</rights><rights>African Association of Insect Scientists 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-294a992ae91ccf987da94d2bf960da5d1ca007d9c98eb87817490a499957fdfc3</citedby><cites>FETCH-LOGICAL-c319t-294a992ae91ccf987da94d2bf960da5d1ca007d9c98eb87817490a499957fdfc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42690-020-00201-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s42690-020-00201-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Varada, Burdekar</creatorcontrib><creatorcontrib>Pradeep, Appukuttan Nair R.</creatorcontrib><creatorcontrib>Awasthi, Arvind K.</creatorcontrib><creatorcontrib>Ponnuvel, Kangayam M.</creatorcontrib><title>Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm</title><title>International journal of tropical insect science</title><addtitle>Int J Trop Insect Sci</addtitle><description>Infections due to
Bombyx mori
nucleopolyhedro virus (
Bm
NPV) in commercially important silkworm,
Bombyx mori
L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in
B. mori
. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic
B. mori
. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of
gp-41
gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes,
ie-1, lef1
and
p74
enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes.</description><subject>Antiviral activity</subject><subject>Biomedical and Life Sciences</subject><subject>Bombyx mori</subject><subject>Copy number</subject><subject>Double-stranded RNA</subject><subject>Entomology</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Inbreeding</subject><subject>Larvae</subject><subject>Life Sciences</subject><subject>Multiplication</subject><subject>Original Research Article</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Signs and symptoms</subject><subject>Silk</subject><subject>Silkworms</subject><subject>Transgenes</subject><subject>Viruses</subject><issn>1742-7592</issn><issn>1742-7584</issn><issn>1742-7592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9UE1LAzEQDaJgrf4BTwHPq0l2280cS_ELahVRryGbZEvqNrsmqbX99aZW0ZOQyQwz771hHkKnlJxTQsqLULAhkIywFOmj2WYP9WhZsKwcANv_Ux-ioxDmhOQ057SHVnetXjYy2tbhtsbThxc8M85g89F5E8K23ckYjXdYOo29icb9gB-nI5vhSgaj0zDad-tlg6XaVnGNrUuv8mkYvXQhyVqFg21eV61fHKODWjbBnHznPnq-unwa32ST--vb8WiSqZxCzBgUEoBJA1SpGnipJRSaVTUMiZYDTZVM52tQwE3FS57OBCILABiUta5V3kdnO93Ot29LE6KYt0vv0krBinzIgXFOE4rtUMq3IXhTi87bhfRrQYnYGix2BovkrfgyWGwSKd-RQgK7mfG_0v-wPgFRIoB7</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Varada, Burdekar</creator><creator>Pradeep, Appukuttan Nair R.</creator><creator>Awasthi, Arvind K.</creator><creator>Ponnuvel, Kangayam M.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>2020</creationdate><title>Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm</title><author>Varada, Burdekar ; Pradeep, Appukuttan Nair R. ; Awasthi, Arvind K. ; Ponnuvel, Kangayam M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-294a992ae91ccf987da94d2bf960da5d1ca007d9c98eb87817490a499957fdfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antiviral activity</topic><topic>Biomedical and Life Sciences</topic><topic>Bombyx mori</topic><topic>Copy number</topic><topic>Double-stranded RNA</topic><topic>Entomology</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Inbreeding</topic><topic>Larvae</topic><topic>Life Sciences</topic><topic>Multiplication</topic><topic>Original Research Article</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Signs and symptoms</topic><topic>Silk</topic><topic>Silkworms</topic><topic>Transgenes</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Varada, Burdekar</creatorcontrib><creatorcontrib>Pradeep, Appukuttan Nair R.</creatorcontrib><creatorcontrib>Awasthi, Arvind K.</creatorcontrib><creatorcontrib>Ponnuvel, Kangayam M.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of tropical insect science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Varada, Burdekar</au><au>Pradeep, Appukuttan Nair R.</au><au>Awasthi, Arvind K.</au><au>Ponnuvel, Kangayam M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm</atitle><jtitle>International journal of tropical insect science</jtitle><stitle>Int J Trop Insect Sci</stitle><date>2020</date><risdate>2020</risdate><volume>40</volume><issue>3</issue><spage>483</spage><epage>491</epage><pages>483-491</pages><issn>1742-7592</issn><issn>1742-7584</issn><eissn>1742-7592</eissn><abstract>Infections due to
Bombyx mori
nucleopolyhedro virus (
Bm
NPV) in commercially important silkworm,
Bombyx mori
L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in
B. mori
. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic
B. mori
. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of
gp-41
gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes,
ie-1, lef1
and
p74
enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s42690-020-00201-z</doi><tpages>9</tpages></addata></record> |
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| ispartof | International journal of tropical insect science, 2020, Vol.40 (3), p.483-491 |
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| subjects | Antiviral activity Biomedical and Life Sciences Bombyx mori Copy number Double-stranded RNA Entomology Gene expression Genes Inbreeding Larvae Life Sciences Multiplication Original Research Article Ribonucleic acid RNA RNA-mediated interference Signs and symptoms Silk Silkworms Transgenes Viruses |
| title | Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
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