Formulation and Evaluation of Nano-fiber-based Transdermal patch of Cephalexin

The nano-fibers were prepared by electro spinning technique by using polyvinyl alcohol (PVA) as a polymer and fibers were characterized on the basis of entrapment efficiency, fiber diameter, morphology, x-ray diffraction analysis, drug release behavior and in vitro antimicrobial test. The final solution was subjected to electro spinning using rotating cylindrical drum as a collector placed at a distance of 20cm from the needle under an applied voltage of 20 KV and flow rate of 0.3 ml/h. Surface morphology and fiber diameter of the optimized nano-fibers was analyzed by using scanning electron microscope (SEM)6. The receptor compartment was filled with 20 ml of phosphate buffer pH 7.4 and maintained at 37°C±0.5°C. Optimized nanofiber sample was kept in donor compartment over a dialysis membrane.