Active-modulated, random-illumination, super-resolution optical fluctuation imaging
Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations. However, conventional SOFI imaging relies on the intrinsic blinking properties of fluorescent markers and suffers from severe artifacts and sign...
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| Veröffentlicht in: | Nanoscale 2020-08, Vol.12 (32), p.16864-16874 |
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| creator | Wang, Baoju Liu, Zhijia Zhou, Li Fei, Yiyan Yang, Chengliang Mi, Lan Mu, Quanquan Ma, Jiong |
| description | Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations. However, conventional SOFI imaging relies on the intrinsic blinking properties of fluorescent markers and suffers from severe artifacts and signal losses owing to the unmatched blinking on-time ratio. Herein, we propose active-modulated, random-illumination, super-resolution optical fluctuation imaging that allows the traditional SOFI to overcome the effect of the intrinsic impertinent blinking characteristic of fluorescent markers. We demonstrate theoretically and experimentally that this method of active-modulated random illumination can generate random illumination patterns with a controllable blinking on-time ratio to match the high-order SOFI reconstruction considerably reducing the generated artifacts and signal losses. High-order, high-quality images can be obtained with increased lateral resolution.
Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations. |
| doi_str_mv | 10.1039/d0nr03255g |
| format | Article |
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Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations.</description><subject>Blinking</subject><subject>Fluorescent indicators</subject><subject>Illumination</subject><subject>Image quality</subject><subject>Image reconstruction</subject><subject>Image resolution</subject><subject>Markers</subject><subject>Signal quality</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp90M9LwzAUB_AgCs7pxbtQ8SKyatIkbXMcU6cwFPxxLmmajIy0qUkj-N-bbTLBg-TwwnsfwssXgFMErxHE7KaBnYM4o3S5B0YZJDDFuMj2d_ecHIIj71cQ5gzneARep2LQnzJtbRMMH2QzSRzvGtum2pjQ6o4P2naTxIdeutRJb01YdxLbD1pwkygTxBA2KtEtX-pueQwOFDdenvzUMXi_v3ubPaSL5_njbLpIBSZwSEkR92Q8lw2VvKQKEVaXQhWMk0wwihEUtFas5kRFVaKMKFSyuoaINLRABI_B5fbd3tmPIP1QtdoLaQzvpA2-yghGZTwFi_TiD13Z4Lq43VpRinKa0aiutko4672Tqupd_JP7qhCs1vlWt_DpZZPvPOKzLXZe7Nxv_nF-_t-86huFvwH7dYLl</recordid><startdate>20200820</startdate><enddate>20200820</enddate><creator>Wang, Baoju</creator><creator>Liu, Zhijia</creator><creator>Zhou, Li</creator><creator>Fei, Yiyan</creator><creator>Yang, Chengliang</creator><creator>Mi, Lan</creator><creator>Mu, Quanquan</creator><creator>Ma, Jiong</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3632-4624</orcidid><orcidid>https://orcid.org/0000-0002-2320-2225</orcidid></search><sort><creationdate>20200820</creationdate><title>Active-modulated, random-illumination, super-resolution optical fluctuation imaging</title><author>Wang, Baoju ; Liu, Zhijia ; Zhou, Li ; Fei, Yiyan ; Yang, Chengliang ; Mi, Lan ; Mu, Quanquan ; Ma, Jiong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-472559a6ed5ea85f149b8cf79a42c95310c5bf9ba4f9a68124f189bb014d57143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Blinking</topic><topic>Fluorescent indicators</topic><topic>Illumination</topic><topic>Image quality</topic><topic>Image reconstruction</topic><topic>Image resolution</topic><topic>Markers</topic><topic>Signal quality</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Baoju</creatorcontrib><creatorcontrib>Liu, Zhijia</creatorcontrib><creatorcontrib>Zhou, Li</creatorcontrib><creatorcontrib>Fei, Yiyan</creatorcontrib><creatorcontrib>Yang, Chengliang</creatorcontrib><creatorcontrib>Mi, Lan</creatorcontrib><creatorcontrib>Mu, Quanquan</creatorcontrib><creatorcontrib>Ma, Jiong</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Baoju</au><au>Liu, Zhijia</au><au>Zhou, Li</au><au>Fei, Yiyan</au><au>Yang, Chengliang</au><au>Mi, Lan</au><au>Mu, Quanquan</au><au>Ma, Jiong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Active-modulated, random-illumination, super-resolution optical fluctuation imaging</atitle><jtitle>Nanoscale</jtitle><date>2020-08-20</date><risdate>2020</risdate><volume>12</volume><issue>32</issue><spage>16864</spage><epage>16874</epage><pages>16864-16874</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations. However, conventional SOFI imaging relies on the intrinsic blinking properties of fluorescent markers and suffers from severe artifacts and signal losses owing to the unmatched blinking on-time ratio. Herein, we propose active-modulated, random-illumination, super-resolution optical fluctuation imaging that allows the traditional SOFI to overcome the effect of the intrinsic impertinent blinking characteristic of fluorescent markers. We demonstrate theoretically and experimentally that this method of active-modulated random illumination can generate random illumination patterns with a controllable blinking on-time ratio to match the high-order SOFI reconstruction considerably reducing the generated artifacts and signal losses. High-order, high-quality images can be obtained with increased lateral resolution.
Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d0nr03255g</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3632-4624</orcidid><orcidid>https://orcid.org/0000-0002-2320-2225</orcidid></addata></record> |
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| identifier | ISSN: 2040-3364 |
| ispartof | Nanoscale, 2020-08, Vol.12 (32), p.16864-16874 |
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| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Blinking Fluorescent indicators Illumination Image quality Image reconstruction Image resolution Markers Signal quality |
| title | Active-modulated, random-illumination, super-resolution optical fluctuation imaging |
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