The Expression of Cytoplasmic and Membrane Proteins in Dog Adipose-Derived Stem Cells on Different Passages During Cultivation in Vitro

ABSTRACT The objective of the study was immunophenotypic analysis of mesenchymal stem cells from dog adipose tissue during in vitro cultivation. Materials and Methods: Mesenchymal stem cells were obtained from dog adipose tissue by explant method. The cells were cultured in CO incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IVth and Xth passages was examined by immunohistochemical method using monoclonal antibodies. Determination the index of proliferation dogs adipose-derived stem cells(DADSCs) on IVth and Xth passages. Established that DADSCs contains multipotent stem cells, that are characterized by an almost homogenous fibroblast-like cells n the IVth and th passages. It was difined, that fibroblast-like cells n the IVth and th passages was expressed cytoplasmic and membrane proteins, which are specific to proliferating cells. Proliferation index in adipose-derived stem cells culture was significantly greater at the IVth passage - 1.90±0.02, compared to cells the Xth passage - 1,52±0,04 **(P