Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia
Entamoeba suis and E . polecki subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196...
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| Veröffentlicht in: | Parasitology research (1987) 2020-09, Vol.119 (9), p.2983-2990 |
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| creator | Wardhana, April Hari Sawitri, Dyah Haryuningtyas Ekawasti, Fitrine Martindah, Eny Apritadewi, Dias Shibahara, Tomoyuki Kusumoto, Masahiro Tokoro, Masaharu Sasai, Kazumi Matsubayashi, Makoto |
| description | Entamoeba suis
and
E
.
polecki
subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine
Entamoeba
-specific primers.
E
.
suis
was the more frequently detected species, observed in 81.1% of samples, while
E
.
polecki
ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of
Entamoeba
) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of
E
.
polecki
ST3. The prevalences of
Eimeria
spp. and/or
Cystoisospora suis
(79.1%), strongyles (55.6%), and
Strongyloides
spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the
Entamoeba
parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms. |
| doi_str_mv | 10.1007/s00436-020-06806-0 |
| format | Article |
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and
E
.
polecki
subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine
Entamoeba
-specific primers.
E
.
suis
was the more frequently detected species, observed in 81.1% of samples, while
E
.
polecki
ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of
Entamoeba
) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of
E
.
polecki
ST3. The prevalences of
Eimeria
spp. and/or
Cystoisospora suis
(79.1%), strongyles (55.6%), and
Strongyloides
spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the
Entamoeba
parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-020-06806-0</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Entamoeba ; Entamoeba polecki ; Epidemiology ; Health aspects ; Hogs ; Immunology ; Infection ; Medical Microbiology ; Microbiology ; Prevalence studies (Epidemiology) ; Protozoology - Original Paper ; Species ; Statistical analysis ; Swine ; Virulence ; Virulence (Microbiology)</subject><ispartof>Parasitology research (1987), 2020-09, Vol.119 (9), p.2983-2990</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-3d05a9f8798a7d1d0d6135fc2eb3825cdb1c5de29069a0d100546b5adae789bf3</citedby><cites>FETCH-LOGICAL-c419t-3d05a9f8798a7d1d0d6135fc2eb3825cdb1c5de29069a0d100546b5adae789bf3</cites><orcidid>0000-0003-3692-8946</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-020-06806-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-020-06806-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Wardhana, April Hari</creatorcontrib><creatorcontrib>Sawitri, Dyah Haryuningtyas</creatorcontrib><creatorcontrib>Ekawasti, Fitrine</creatorcontrib><creatorcontrib>Martindah, Eny</creatorcontrib><creatorcontrib>Apritadewi, Dias</creatorcontrib><creatorcontrib>Shibahara, Tomoyuki</creatorcontrib><creatorcontrib>Kusumoto, Masahiro</creatorcontrib><creatorcontrib>Tokoro, Masaharu</creatorcontrib><creatorcontrib>Sasai, Kazumi</creatorcontrib><creatorcontrib>Matsubayashi, Makoto</creatorcontrib><title>Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>Entamoeba suis
and
E
.
polecki
subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine
Entamoeba
-specific primers.
E
.
suis
was the more frequently detected species, observed in 81.1% of samples, while
E
.
polecki
ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of
Entamoeba
) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of
E
.
polecki
ST3. The prevalences of
Eimeria
spp. and/or
Cystoisospora suis
(79.1%), strongyles (55.6%), and
Strongyloides
spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the
Entamoeba
parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Entamoeba</subject><subject>Entamoeba polecki</subject><subject>Epidemiology</subject><subject>Health aspects</subject><subject>Hogs</subject><subject>Immunology</subject><subject>Infection</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Protozoology - Original Paper</subject><subject>Species</subject><subject>Statistical analysis</subject><subject>Swine</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kVFLHDEUhUNpwa3tH_Ap0FdnvZlMZiaPImurCL4ofQx3kpsldjfZJrOC_77RLYggEkgO4Xw3N_cwdiJgKQCGswLQyb6BFhroR6jqE1uITraN0Ep9ZgvQVYMQ8oh9LeUBQAx91y3Y0621-5wpWuIYHV9TpDlYHhzFOfhgcQ4pFp4836VsQyS-ijNuE014yldLXvahvJBV79KG7J9wynHmdxjXlOvGQ-S_qcz8Gh8rchVdilQCfmNfPG4Kff9_HrP7y9Xdxa_m5vbn1cX5TWM7oedGOlCo_TjoEQcnHLheSOVtS5McW2XdJKxy1GroNYKr01BdPyl0SMOoJy-P2Y9D3V1Of_e1EfOQ9jnWJ03byW6Uus7p1bXGDZkQfZoz2m0o1pz3sh2FUmNfXct3XHU52gZb_-VDvX8DtAfA5lRKJm92OWwxPxkB5jk5c0jO1OTMS3IGKiQPUKnm5ym-dvwB9Q8j5pmh</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Wardhana, April Hari</creator><creator>Sawitri, Dyah Haryuningtyas</creator><creator>Ekawasti, Fitrine</creator><creator>Martindah, Eny</creator><creator>Apritadewi, Dias</creator><creator>Shibahara, Tomoyuki</creator><creator>Kusumoto, Masahiro</creator><creator>Tokoro, Masaharu</creator><creator>Sasai, Kazumi</creator><creator>Matsubayashi, Makoto</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-3692-8946</orcidid></search><sort><creationdate>20200901</creationdate><title>Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia</title><author>Wardhana, April Hari ; Sawitri, Dyah Haryuningtyas ; Ekawasti, Fitrine ; Martindah, Eny ; Apritadewi, Dias ; Shibahara, Tomoyuki ; Kusumoto, Masahiro ; Tokoro, Masaharu ; Sasai, Kazumi ; Matsubayashi, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-3d05a9f8798a7d1d0d6135fc2eb3825cdb1c5de29069a0d100546b5adae789bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Entamoeba</topic><topic>Entamoeba polecki</topic><topic>Epidemiology</topic><topic>Health aspects</topic><topic>Hogs</topic><topic>Immunology</topic><topic>Infection</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Protozoology - Original Paper</topic><topic>Species</topic><topic>Statistical analysis</topic><topic>Swine</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wardhana, April Hari</creatorcontrib><creatorcontrib>Sawitri, Dyah Haryuningtyas</creatorcontrib><creatorcontrib>Ekawasti, Fitrine</creatorcontrib><creatorcontrib>Martindah, Eny</creatorcontrib><creatorcontrib>Apritadewi, Dias</creatorcontrib><creatorcontrib>Shibahara, Tomoyuki</creatorcontrib><creatorcontrib>Kusumoto, Masahiro</creatorcontrib><creatorcontrib>Tokoro, Masaharu</creatorcontrib><creatorcontrib>Sasai, Kazumi</creatorcontrib><creatorcontrib>Matsubayashi, Makoto</creatorcontrib><collection>CrossRef</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wardhana, April Hari</au><au>Sawitri, Dyah Haryuningtyas</au><au>Ekawasti, Fitrine</au><au>Martindah, Eny</au><au>Apritadewi, Dias</au><au>Shibahara, Tomoyuki</au><au>Kusumoto, Masahiro</au><au>Tokoro, Masaharu</au><au>Sasai, Kazumi</au><au>Matsubayashi, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>119</volume><issue>9</issue><spage>2983</spage><epage>2990</epage><pages>2983-2990</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Entamoeba suis
and
E
.
polecki
subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine
Entamoeba
-specific primers.
E
.
suis
was the more frequently detected species, observed in 81.1% of samples, while
E
.
polecki
ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of
Entamoeba
) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of
E
.
polecki
ST3. The prevalences of
Eimeria
spp. and/or
Cystoisospora suis
(79.1%), strongyles (55.6%), and
Strongyloides
spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the
Entamoeba
parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00436-020-06806-0</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3692-8946</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2020-09, Vol.119 (9), p.2983-2990 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_journals_2434839143 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Biomedicine Entamoeba Entamoeba polecki Epidemiology Health aspects Hogs Immunology Infection Medical Microbiology Microbiology Prevalence studies (Epidemiology) Protozoology - Original Paper Species Statistical analysis Swine Virulence Virulence (Microbiology) |
| title | Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia |
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