Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway
Carnitine palmitoyltransferase 1A (CPT1A) is a potential therapeutic target for several cancers. The inhibition of CPT1A has anticancer effects. Teglicar is a new reversible CPT1A inhibitor. The aim of this research was to expound the effect of teglicar in cervical cancer cells. Cell proliferation w...
Gespeichert in:
| Veröffentlicht in: | Iranian journal of science and technology. Transaction A, Science Science, 2020-08, Vol.44 (4), p.973-979 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 979 |
|---|---|
| container_issue | 4 |
| container_start_page | 973 |
| container_title | Iranian journal of science and technology. Transaction A, Science |
| container_volume | 44 |
| creator | Zhang, Rui Nie, Dunli Chen, Yan Zeng, Yunlan Huang, Yu Xiao, Ning |
| description | Carnitine palmitoyltransferase 1A (CPT1A) is a potential therapeutic target for several cancers. The inhibition of CPT1A has anticancer effects. Teglicar is a new reversible CPT1A inhibitor. The aim of this research was to expound the effect of teglicar in cervical cancer cells. Cell proliferation was conducted using the CCK8 assay. The apoptotic rate was examined by Annexin V-APC/PI staining and flow cytometry. The expression levels of apoptosis-related proteins and endoplasmic reticulum (ER) stress-related proteins were determined by western blot assay. Teglicar was found to inhibit the proliferation of HeLa cells in a dose-dependent manner, with an IC
50
value of 18.38 µM at 24 h. The apoptotic rate of HeLa cells was significantly increased following treatment with teglicar. Western blot analysis indicated that the treatment of teglicar enhanced ER stress and apoptosis in HeLa cells. However, inhibition of proliferation and enhancement of apoptosis in teglicar-treated HeLa cells was reversed by 4-PBA. These results revealed that teglicar promoted apoptosis of HeLa cells via ER stress. |
| doi_str_mv | 10.1007/s40995-020-00940-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2429456183</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2429456183</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-f28eb2638b8d6556d370ded90487fd9a8596e909bb3d034d89051bf93620a5123</originalsourceid><addsrcrecordid>eNp9kFFLwzAUhYMoOOb-gE8Bn6M3SZsmj2NMN5goOp9D2qQzo2tr0k72761W8M2ny4HvnAsfQtcUbilAdhcTUColwIAAqAQIPUMTxkVCqKTqHE0oMEkEy8QlmsXoc-CUiowlYoL2W7erfGECXte2L1zE87Zpuyb6iH2NV25j8MKF44BUeGHqwoUhV1XER2_wsrZNW5l48AV-cZ0v-qo_4NcuuBjJo7PedM7iZ9O9f5rTFbooTRXd7PdO0dv9crtYkc3Tw3ox35CCU9WRkkmXM8FlLq1IU2F5BtZZBYnMSquMTJVwClSecws8sVJBSvNSccHApJTxKboZd9vQfPQudnrf9KEeXmqWMJWkgko-UGykitDEGFyp2-APJpw0Bf2tVY9a9aBV_2jVdCjxsRQHuN658Df9T-sLUD56Fg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2429456183</pqid></control><display><type>article</type><title>Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway</title><source>Alma/SFX Local Collection</source><creator>Zhang, Rui ; Nie, Dunli ; Chen, Yan ; Zeng, Yunlan ; Huang, Yu ; Xiao, Ning</creator><creatorcontrib>Zhang, Rui ; Nie, Dunli ; Chen, Yan ; Zeng, Yunlan ; Huang, Yu ; Xiao, Ning</creatorcontrib><description>Carnitine palmitoyltransferase 1A (CPT1A) is a potential therapeutic target for several cancers. The inhibition of CPT1A has anticancer effects. Teglicar is a new reversible CPT1A inhibitor. The aim of this research was to expound the effect of teglicar in cervical cancer cells. Cell proliferation was conducted using the CCK8 assay. The apoptotic rate was examined by Annexin V-APC/PI staining and flow cytometry. The expression levels of apoptosis-related proteins and endoplasmic reticulum (ER) stress-related proteins were determined by western blot assay. Teglicar was found to inhibit the proliferation of HeLa cells in a dose-dependent manner, with an IC
50
value of 18.38 µM at 24 h. The apoptotic rate of HeLa cells was significantly increased following treatment with teglicar. Western blot analysis indicated that the treatment of teglicar enhanced ER stress and apoptosis in HeLa cells. However, inhibition of proliferation and enhancement of apoptosis in teglicar-treated HeLa cells was reversed by 4-PBA. These results revealed that teglicar promoted apoptosis of HeLa cells via ER stress.</description><identifier>ISSN: 1028-6276</identifier><identifier>EISSN: 2364-1819</identifier><identifier>DOI: 10.1007/s40995-020-00940-1</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adenomatous polyposis coli ; Annexin V ; Anticancer properties ; Apoptosis ; Biology ; Carnitine ; Carnitine palmitoyltransferase ; Cell proliferation ; Cervical cancer ; Cervix ; Chemistry/Food Science ; Earth Sciences ; Endoplasmic reticulum ; Engineering ; Flow cytometry ; HeLa cells ; Life Sciences ; Materials Science ; Palmitoyltransferase ; Physics ; Proteins ; Research Paper ; Stress</subject><ispartof>Iranian journal of science and technology. Transaction A, Science, 2020-08, Vol.44 (4), p.973-979</ispartof><rights>Shiraz University 2020</rights><rights>Shiraz University 2020.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-f28eb2638b8d6556d370ded90487fd9a8596e909bb3d034d89051bf93620a5123</citedby><cites>FETCH-LOGICAL-c319t-f28eb2638b8d6556d370ded90487fd9a8596e909bb3d034d89051bf93620a5123</cites><orcidid>0000-0002-9315-4417</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Nie, Dunli</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><creatorcontrib>Zeng, Yunlan</creatorcontrib><creatorcontrib>Huang, Yu</creatorcontrib><creatorcontrib>Xiao, Ning</creatorcontrib><title>Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway</title><title>Iranian journal of science and technology. Transaction A, Science</title><addtitle>Iran J Sci Technol Trans Sci</addtitle><description>Carnitine palmitoyltransferase 1A (CPT1A) is a potential therapeutic target for several cancers. The inhibition of CPT1A has anticancer effects. Teglicar is a new reversible CPT1A inhibitor. The aim of this research was to expound the effect of teglicar in cervical cancer cells. Cell proliferation was conducted using the CCK8 assay. The apoptotic rate was examined by Annexin V-APC/PI staining and flow cytometry. The expression levels of apoptosis-related proteins and endoplasmic reticulum (ER) stress-related proteins were determined by western blot assay. Teglicar was found to inhibit the proliferation of HeLa cells in a dose-dependent manner, with an IC
50
value of 18.38 µM at 24 h. The apoptotic rate of HeLa cells was significantly increased following treatment with teglicar. Western blot analysis indicated that the treatment of teglicar enhanced ER stress and apoptosis in HeLa cells. However, inhibition of proliferation and enhancement of apoptosis in teglicar-treated HeLa cells was reversed by 4-PBA. These results revealed that teglicar promoted apoptosis of HeLa cells via ER stress.</description><subject>Adenomatous polyposis coli</subject><subject>Annexin V</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Biology</subject><subject>Carnitine</subject><subject>Carnitine palmitoyltransferase</subject><subject>Cell proliferation</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Chemistry/Food Science</subject><subject>Earth Sciences</subject><subject>Endoplasmic reticulum</subject><subject>Engineering</subject><subject>Flow cytometry</subject><subject>HeLa cells</subject><subject>Life Sciences</subject><subject>Materials Science</subject><subject>Palmitoyltransferase</subject><subject>Physics</subject><subject>Proteins</subject><subject>Research Paper</subject><subject>Stress</subject><issn>1028-6276</issn><issn>2364-1819</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kFFLwzAUhYMoOOb-gE8Bn6M3SZsmj2NMN5goOp9D2qQzo2tr0k72761W8M2ny4HvnAsfQtcUbilAdhcTUColwIAAqAQIPUMTxkVCqKTqHE0oMEkEy8QlmsXoc-CUiowlYoL2W7erfGECXte2L1zE87Zpuyb6iH2NV25j8MKF44BUeGHqwoUhV1XER2_wsrZNW5l48AV-cZ0v-qo_4NcuuBjJo7PedM7iZ9O9f5rTFbooTRXd7PdO0dv9crtYkc3Tw3ox35CCU9WRkkmXM8FlLq1IU2F5BtZZBYnMSquMTJVwClSecws8sVJBSvNSccHApJTxKboZd9vQfPQudnrf9KEeXmqWMJWkgko-UGykitDEGFyp2-APJpw0Bf2tVY9a9aBV_2jVdCjxsRQHuN658Df9T-sLUD56Fg</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Zhang, Rui</creator><creator>Nie, Dunli</creator><creator>Chen, Yan</creator><creator>Zeng, Yunlan</creator><creator>Huang, Yu</creator><creator>Xiao, Ning</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SC</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>H8D</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><orcidid>https://orcid.org/0000-0002-9315-4417</orcidid></search><sort><creationdate>20200801</creationdate><title>Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway</title><author>Zhang, Rui ; Nie, Dunli ; Chen, Yan ; Zeng, Yunlan ; Huang, Yu ; Xiao, Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-f28eb2638b8d6556d370ded90487fd9a8596e909bb3d034d89051bf93620a5123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenomatous polyposis coli</topic><topic>Annexin V</topic><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Biology</topic><topic>Carnitine</topic><topic>Carnitine palmitoyltransferase</topic><topic>Cell proliferation</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Chemistry/Food Science</topic><topic>Earth Sciences</topic><topic>Endoplasmic reticulum</topic><topic>Engineering</topic><topic>Flow cytometry</topic><topic>HeLa cells</topic><topic>Life Sciences</topic><topic>Materials Science</topic><topic>Palmitoyltransferase</topic><topic>Physics</topic><topic>Proteins</topic><topic>Research Paper</topic><topic>Stress</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Rui</creatorcontrib><creatorcontrib>Nie, Dunli</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><creatorcontrib>Zeng, Yunlan</creatorcontrib><creatorcontrib>Huang, Yu</creatorcontrib><creatorcontrib>Xiao, Ning</creatorcontrib><collection>CrossRef</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Iranian journal of science and technology. Transaction A, Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Rui</au><au>Nie, Dunli</au><au>Chen, Yan</au><au>Zeng, Yunlan</au><au>Huang, Yu</au><au>Xiao, Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway</atitle><jtitle>Iranian journal of science and technology. Transaction A, Science</jtitle><stitle>Iran J Sci Technol Trans Sci</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>44</volume><issue>4</issue><spage>973</spage><epage>979</epage><pages>973-979</pages><issn>1028-6276</issn><eissn>2364-1819</eissn><abstract>Carnitine palmitoyltransferase 1A (CPT1A) is a potential therapeutic target for several cancers. The inhibition of CPT1A has anticancer effects. Teglicar is a new reversible CPT1A inhibitor. The aim of this research was to expound the effect of teglicar in cervical cancer cells. Cell proliferation was conducted using the CCK8 assay. The apoptotic rate was examined by Annexin V-APC/PI staining and flow cytometry. The expression levels of apoptosis-related proteins and endoplasmic reticulum (ER) stress-related proteins were determined by western blot assay. Teglicar was found to inhibit the proliferation of HeLa cells in a dose-dependent manner, with an IC
50
value of 18.38 µM at 24 h. The apoptotic rate of HeLa cells was significantly increased following treatment with teglicar. Western blot analysis indicated that the treatment of teglicar enhanced ER stress and apoptosis in HeLa cells. However, inhibition of proliferation and enhancement of apoptosis in teglicar-treated HeLa cells was reversed by 4-PBA. These results revealed that teglicar promoted apoptosis of HeLa cells via ER stress.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40995-020-00940-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9315-4417</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1028-6276 |
| ispartof | Iranian journal of science and technology. Transaction A, Science, 2020-08, Vol.44 (4), p.973-979 |
| issn | 1028-6276 2364-1819 |
| language | eng |
| recordid | cdi_proquest_journals_2429456183 |
| source | Alma/SFX Local Collection |
| subjects | Adenomatous polyposis coli Annexin V Anticancer properties Apoptosis Biology Carnitine Carnitine palmitoyltransferase Cell proliferation Cervical cancer Cervix Chemistry/Food Science Earth Sciences Endoplasmic reticulum Engineering Flow cytometry HeLa cells Life Sciences Materials Science Palmitoyltransferase Physics Proteins Research Paper Stress |
| title | Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T08%3A04%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Teglicar%20Induces%20Apoptosis%20in%20HeLa%20Cervical%20Cancer%20Cells%20via%20Endoplasmic%20Reticulum%20Stress-Mediated%20Pathway&rft.jtitle=Iranian%20journal%20of%20science%20and%20technology.%20Transaction%20A,%20Science&rft.au=Zhang,%20Rui&rft.date=2020-08-01&rft.volume=44&rft.issue=4&rft.spage=973&rft.epage=979&rft.pages=973-979&rft.issn=1028-6276&rft.eissn=2364-1819&rft_id=info:doi/10.1007/s40995-020-00940-1&rft_dat=%3Cproquest_cross%3E2429456183%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2429456183&rft_id=info:pmid/&rfr_iscdi=true |