Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples
Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes ( mcr-like ) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to...
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| Veröffentlicht in: | Archives of microbiology 2020-09, Vol.202 (7), p.1795-1800 |
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| creator | dos Santos, Lucas David Rodrigues Furlan, João Pedro Rueda Ramos, Micaela Santana Gallo, Inara Fernanda Lage de Freitas, Lucas Victor Pereira Stehling, Eliana Guedes |
| description | Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes (
mcr-like
) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of
mcr-like
genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs [
mcr-1
,
mcr-3
,
mcr-7.1
,
bla
CTX-M-Gp1
,
bla
CTX-M-Gp2
,
bla
CTX-M-Gp9
,
bla
VEB
,
bla
PER
,
bla
CMY
,
tetA, tetB, tetC, aadA, aac(6′)-Ib, aph(3′)-Ia, ant(2′')-Ia
,
qnrA
,
qnrB, qnrS, oqxA, oqxB
,
sul1, sul2, sul3, cmlA, mefAE
,
ermB
] and 21 amplicons from eight plasmid families (IncY, ColE-
like
, IncF
repB
, IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including
mcr-like
, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of
mcr-1
,
mcr-3
and
mcr-7.1
in environmental samples from the zoo. |
| doi_str_mv | 10.1007/s00203-020-01890-3 |
| format | Article |
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mcr-like
) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of
mcr-like
genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs [
mcr-1
,
mcr-3
,
mcr-7.1
,
bla
CTX-M-Gp1
,
bla
CTX-M-Gp2
,
bla
CTX-M-Gp9
,
bla
VEB
,
bla
PER
,
bla
CMY
,
tetA, tetB, tetC, aadA, aac(6′)-Ib, aph(3′)-Ia, ant(2′')-Ia
,
qnrA
,
qnrB, qnrS, oqxA, oqxB
,
sul1, sul2, sul3, cmlA, mefAE
,
ermB
] and 21 amplicons from eight plasmid families (IncY, ColE-
like
, IncF
repB
, IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including
mcr-like
, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of
mcr-1
,
mcr-3
and
mcr-7.1
in environmental samples from the zoo.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-020-01890-3</identifier><identifier>PMID: 32382766</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aerobic bacteria ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Colistin ; Deoxyribonucleic acid ; DNA ; Drug resistance ; Ecology ; Genes ; Life Sciences ; Microbial Ecology ; Microbiology ; Multidrug resistance ; Original Paper ; Plasmids ; Polymerase chain reaction ; Soil water</subject><ispartof>Archives of microbiology, 2020-09, Vol.202 (7), p.1795-1800</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-92ccec1d0e22a58de467eb55ea61d39f00c685efa80bcf27f18f6a1ecd600e293</citedby><cites>FETCH-LOGICAL-c419t-92ccec1d0e22a58de467eb55ea61d39f00c685efa80bcf27f18f6a1ecd600e293</cites><orcidid>0000-0003-2230-9028 ; 0000-0002-4362-3203 ; 0000-0003-2516-1129</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-020-01890-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-020-01890-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32382766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>dos Santos, Lucas David Rodrigues</creatorcontrib><creatorcontrib>Furlan, João Pedro Rueda</creatorcontrib><creatorcontrib>Ramos, Micaela Santana</creatorcontrib><creatorcontrib>Gallo, Inara Fernanda Lage</creatorcontrib><creatorcontrib>de Freitas, Lucas Victor Pereira</creatorcontrib><creatorcontrib>Stehling, Eliana Guedes</creatorcontrib><title>Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes (
mcr-like
) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of
mcr-like
genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs [
mcr-1
,
mcr-3
,
mcr-7.1
,
bla
CTX-M-Gp1
,
bla
CTX-M-Gp2
,
bla
CTX-M-Gp9
,
bla
VEB
,
bla
PER
,
bla
CMY
,
tetA, tetB, tetC, aadA, aac(6′)-Ib, aph(3′)-Ia, ant(2′')-Ia
,
qnrA
,
qnrB, qnrS, oqxA, oqxB
,
sul1, sul2, sul3, cmlA, mefAE
,
ermB
] and 21 amplicons from eight plasmid families (IncY, ColE-
like
, IncF
repB
, IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including
mcr-like
, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of
mcr-1
,
mcr-3
and
mcr-7.1
in environmental samples from the zoo.</description><subject>Aerobic bacteria</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Colistin</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Ecology</subject><subject>Genes</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Original Paper</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Soil water</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEtLAzEUhYMotj7-gAsZcGv0JmknmaUUX1Bwo-AupJkbicxkajItdONvN-342Lm5B27O-S45hJwxuGIA8joBcBA0DwpMVUDFHhmzieAUJH_dJ2MQwKmqhBiRo5TeARhXSh2SkeBCcVmWY_I562hn7SpGDBaLzhWtjZRd7kQMIgsT6sI2PnhrmmZTRGxwbUKf971vvY3dwpsmr5NPvdly3jBgKnwoMKx97EKLoc-OLcdhhhTJtMsG0wk5cKZJePqtx-Tl7vZ59kDnT_ePs5s5tRNW9bTi1qJlNSDnZqpqnJQSF9MpmpLVonIAtlRTdEbBwjouHVOuNAxtXULOVOKYXAzcZew-Vph6_d6tYsgnNZ9wKSqQcuvigyv_KKWITi-jb03caAZ6W7keKtd56F3lWuTQ-Td6tWix_o38dJwNYjCk_BTeMP7d_gf7BaiKjVs</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>dos Santos, Lucas David Rodrigues</creator><creator>Furlan, João Pedro Rueda</creator><creator>Ramos, Micaela Santana</creator><creator>Gallo, Inara Fernanda Lage</creator><creator>de Freitas, Lucas Victor Pereira</creator><creator>Stehling, Eliana Guedes</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-2230-9028</orcidid><orcidid>https://orcid.org/0000-0002-4362-3203</orcidid><orcidid>https://orcid.org/0000-0003-2516-1129</orcidid></search><sort><creationdate>20200901</creationdate><title>Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples</title><author>dos Santos, Lucas David Rodrigues ; Furlan, João Pedro Rueda ; Ramos, Micaela Santana ; Gallo, Inara Fernanda Lage ; de Freitas, Lucas Victor Pereira ; Stehling, Eliana Guedes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-92ccec1d0e22a58de467eb55ea61d39f00c685efa80bcf27f18f6a1ecd600e293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aerobic bacteria</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Colistin</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Ecology</topic><topic>Genes</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Original Paper</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Soil water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>dos Santos, Lucas David Rodrigues</creatorcontrib><creatorcontrib>Furlan, João Pedro Rueda</creatorcontrib><creatorcontrib>Ramos, Micaela Santana</creatorcontrib><creatorcontrib>Gallo, Inara Fernanda Lage</creatorcontrib><creatorcontrib>de Freitas, Lucas Victor Pereira</creatorcontrib><creatorcontrib>Stehling, Eliana Guedes</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>dos Santos, Lucas David Rodrigues</au><au>Furlan, João Pedro Rueda</au><au>Ramos, Micaela Santana</au><au>Gallo, Inara Fernanda Lage</au><au>de Freitas, Lucas Victor Pereira</au><au>Stehling, Eliana Guedes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>202</volume><issue>7</issue><spage>1795</spage><epage>1800</epage><pages>1795-1800</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes (
mcr-like
) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of
mcr-like
genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs [
mcr-1
,
mcr-3
,
mcr-7.1
,
bla
CTX-M-Gp1
,
bla
CTX-M-Gp2
,
bla
CTX-M-Gp9
,
bla
VEB
,
bla
PER
,
bla
CMY
,
tetA, tetB, tetC, aadA, aac(6′)-Ib, aph(3′)-Ia, ant(2′')-Ia
,
qnrA
,
qnrB, qnrS, oqxA, oqxB
,
sul1, sul2, sul3, cmlA, mefAE
,
ermB
] and 21 amplicons from eight plasmid families (IncY, ColE-
like
, IncF
repB
, IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including
mcr-like
, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of
mcr-1
,
mcr-3
and
mcr-7.1
in environmental samples from the zoo.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32382766</pmid><doi>10.1007/s00203-020-01890-3</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-2230-9028</orcidid><orcidid>https://orcid.org/0000-0002-4362-3203</orcidid><orcidid>https://orcid.org/0000-0003-2516-1129</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Archives of microbiology, 2020-09, Vol.202 (7), p.1795-1800 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_journals_2427390779 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Aerobic bacteria Antimicrobial agents Antimicrobial resistance Bacteria Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Colistin Deoxyribonucleic acid DNA Drug resistance Ecology Genes Life Sciences Microbial Ecology Microbiology Multidrug resistance Original Paper Plasmids Polymerase chain reaction Soil water |
| title | Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples |
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