Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat
Diabetes increases the possibility of germ cell damage, hypogonadism, and male infertility. Diabetic condition negatively impacts zinc (Zn) and selenium (Se) levels in the body. Zn and Se are among the most important trace elements involved in the regulation of redox reaction, antioxidants enzymes a...
Gespeichert in:
| Veröffentlicht in: | Human & experimental toxicology 2020-09, Vol.39 (9), p.1235-1256 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1256 |
|---|---|
| container_issue | 9 |
| container_start_page | 1235 |
| container_title | Human & experimental toxicology |
| container_volume | 39 |
| creator | Sahu, C Dwivedi, DK Jena, GB |
| description | Diabetes increases the possibility of germ cell damage, hypogonadism, and male infertility. Diabetic condition negatively impacts zinc (Zn) and selenium (Se) levels in the body. Zn and Se are among the most important trace elements involved in the regulation of redox reaction, antioxidants enzymes activities, and DNA expression in a germ cell. The present study aimed to elucidate the combined effects of Zn and Se treatment on diabetes-induced germ cell damage in male Sprague Dawley rats. Type 1 diabetes was induced by the single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) were administered daily for 8 consecutive weeks. All the animals were provided with normal feed and water throughout the study. The effects on germ cell damage were evaluated by body weight, feed-water intake, organ weight, sperm count, motility, sperm head morphology, biochemical analysis, histology, immunohistochemistry, halo assay, germ cell comet assay, testes terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end-labeling (TUNEL) assay, sperm TUNEL assay, serum protein pattern analysis, and subcellular analysis using transmission electron microscopy. Further, the expressions of nuclear erythroid-derived related factor 2, catalase, glutathione peroxidase 4, and glutathione peroxidase 5 were carried out to ascertain the mechanism of protection. The present results demonstrated that 8 weeks combined treatment of Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) reduced diabetes-induced germ cell damage. This study further highlighted that Zn and Se combination treatment might be a better strategy for the germ cell protection in diabetes and deserve further investigation. |
| doi_str_mv | 10.1177/0960327120914963 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_proquest_journals_2425826836</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_0960327120914963</sage_id><sourcerecordid>2425826836</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-44a7a655a384418f404415ed669f75400245f327416f1fbb5d8f07708cf2e9273</originalsourceid><addsrcrecordid>eNp1UE1LxDAQDaK46-rdkwQ8Vyff7VEWv0DwohcvJW0SydKma5oe9t-bdVcFwdMM8968mfcQOidwRYhS11BJYFQRChXhlWQHaE64UgVUwA7RfAsXW3yGTsZxBQCyEuQYzRiljJVQztHqzYcW62DwaDsb_NTjdugbH3TyQ8ApWp16GxJexyHZNlmDjdeNTXYsfDBTmwe5T76dOh2_hOzaG282ve6w0b1-t9gHHHU6RUdOd6M929cFer27fVk-FE_P94_Lm6eiZVKkgnOttBRCs5JzUjoOuQhrpKycEhyAcuGyKU6kI65phCkdKAVl66itqGILdLnTzS9_TPm3ejVMMeSTNeVUlFSWTGYW7FhtHMYxWlevo-913NQE6m249d9w88rFXnhqemt-Fr7TzIRiRxiz69-r_wp-Ak9DgPc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2425826836</pqid></control><display><type>article</type><title>Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat</title><source>Sage Journals GOLD Open Access 2024</source><creator>Sahu, C ; Dwivedi, DK ; Jena, GB</creator><creatorcontrib>Sahu, C ; Dwivedi, DK ; Jena, GB</creatorcontrib><description>Diabetes increases the possibility of germ cell damage, hypogonadism, and male infertility. Diabetic condition negatively impacts zinc (Zn) and selenium (Se) levels in the body. Zn and Se are among the most important trace elements involved in the regulation of redox reaction, antioxidants enzymes activities, and DNA expression in a germ cell. The present study aimed to elucidate the combined effects of Zn and Se treatment on diabetes-induced germ cell damage in male Sprague Dawley rats. Type 1 diabetes was induced by the single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) were administered daily for 8 consecutive weeks. All the animals were provided with normal feed and water throughout the study. The effects on germ cell damage were evaluated by body weight, feed-water intake, organ weight, sperm count, motility, sperm head morphology, biochemical analysis, histology, immunohistochemistry, halo assay, germ cell comet assay, testes terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end-labeling (TUNEL) assay, sperm TUNEL assay, serum protein pattern analysis, and subcellular analysis using transmission electron microscopy. Further, the expressions of nuclear erythroid-derived related factor 2, catalase, glutathione peroxidase 4, and glutathione peroxidase 5 were carried out to ascertain the mechanism of protection. The present results demonstrated that 8 weeks combined treatment of Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) reduced diabetes-induced germ cell damage. This study further highlighted that Zn and Se combination treatment might be a better strategy for the germ cell protection in diabetes and deserve further investigation.</description><identifier>ISSN: 0960-3271</identifier><identifier>EISSN: 1477-0903</identifier><identifier>DOI: 10.1177/0960327120914963</identifier><identifier>PMID: 32233808</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Antioxidants ; Bioassays ; Biochemical analysis ; Body weight ; Catalase ; Catalase - analysis ; Combined treatment ; Comet assay ; Cytology ; Damage assessment ; Damage detection ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (insulin dependent) ; Diabetes Mellitus, Experimental - chemically induced ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Type 1 - chemically induced ; Diabetes Mellitus, Type 1 - complications ; DNA nucleotidylexotransferase ; Epididymis - drug effects ; Epididymis - pathology ; Gene expression ; Glutathione ; Glutathione - analysis ; Glutathione peroxidase ; Histology ; Hypogonadism ; Immunohistochemistry ; Impact damage ; Infertility ; Lipid Peroxidation ; Male ; Morphology ; Organ weight ; Pattern analysis ; Peroxidase ; Rats ; Rats, Sprague-Dawley ; Selenium ; Selenium - pharmacology ; Selenium - therapeutic use ; Serum proteins ; Sperm ; Sperm Count ; Sperm Motility - drug effects ; Spermatozoa - drug effects ; Spermatozoa - pathology ; Streptozocin ; Testis - drug effects ; Testis - pathology ; Toxicity ; Trace elements ; Transmission electron microscopy ; Water intake ; Water intakes ; Zinc ; Zinc - pharmacology ; Zinc - therapeutic use</subject><ispartof>Human & experimental toxicology, 2020-09, Vol.39 (9), p.1235-1256</ispartof><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-44a7a655a384418f404415ed669f75400245f327416f1fbb5d8f07708cf2e9273</citedby><cites>FETCH-LOGICAL-c365t-44a7a655a384418f404415ed669f75400245f327416f1fbb5d8f07708cf2e9273</cites><orcidid>0000-0001-9437-7252</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0960327120914963$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0960327120914963$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21966,27853,27924,27925,44945,45333</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/0960327120914963?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32233808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahu, C</creatorcontrib><creatorcontrib>Dwivedi, DK</creatorcontrib><creatorcontrib>Jena, GB</creatorcontrib><title>Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat</title><title>Human & experimental toxicology</title><addtitle>Hum Exp Toxicol</addtitle><description>Diabetes increases the possibility of germ cell damage, hypogonadism, and male infertility. Diabetic condition negatively impacts zinc (Zn) and selenium (Se) levels in the body. Zn and Se are among the most important trace elements involved in the regulation of redox reaction, antioxidants enzymes activities, and DNA expression in a germ cell. The present study aimed to elucidate the combined effects of Zn and Se treatment on diabetes-induced germ cell damage in male Sprague Dawley rats. Type 1 diabetes was induced by the single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) were administered daily for 8 consecutive weeks. All the animals were provided with normal feed and water throughout the study. The effects on germ cell damage were evaluated by body weight, feed-water intake, organ weight, sperm count, motility, sperm head morphology, biochemical analysis, histology, immunohistochemistry, halo assay, germ cell comet assay, testes terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end-labeling (TUNEL) assay, sperm TUNEL assay, serum protein pattern analysis, and subcellular analysis using transmission electron microscopy. Further, the expressions of nuclear erythroid-derived related factor 2, catalase, glutathione peroxidase 4, and glutathione peroxidase 5 were carried out to ascertain the mechanism of protection. The present results demonstrated that 8 weeks combined treatment of Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) reduced diabetes-induced germ cell damage. This study further highlighted that Zn and Se combination treatment might be a better strategy for the germ cell protection in diabetes and deserve further investigation.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Bioassays</subject><subject>Biochemical analysis</subject><subject>Body weight</subject><subject>Catalase</subject><subject>Catalase - analysis</subject><subject>Combined treatment</subject><subject>Comet assay</subject><subject>Cytology</subject><subject>Damage assessment</subject><subject>Damage detection</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (insulin dependent)</subject><subject>Diabetes Mellitus, Experimental - chemically induced</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Type 1 - chemically induced</subject><subject>Diabetes Mellitus, Type 1 - complications</subject><subject>DNA nucleotidylexotransferase</subject><subject>Epididymis - drug effects</subject><subject>Epididymis - pathology</subject><subject>Gene expression</subject><subject>Glutathione</subject><subject>Glutathione - analysis</subject><subject>Glutathione peroxidase</subject><subject>Histology</subject><subject>Hypogonadism</subject><subject>Immunohistochemistry</subject><subject>Impact damage</subject><subject>Infertility</subject><subject>Lipid Peroxidation</subject><subject>Male</subject><subject>Morphology</subject><subject>Organ weight</subject><subject>Pattern analysis</subject><subject>Peroxidase</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Selenium</subject><subject>Selenium - pharmacology</subject><subject>Selenium - therapeutic use</subject><subject>Serum proteins</subject><subject>Sperm</subject><subject>Sperm Count</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - pathology</subject><subject>Streptozocin</subject><subject>Testis - drug effects</subject><subject>Testis - pathology</subject><subject>Toxicity</subject><subject>Trace elements</subject><subject>Transmission electron microscopy</subject><subject>Water intake</subject><subject>Water intakes</subject><subject>Zinc</subject><subject>Zinc - pharmacology</subject><subject>Zinc - therapeutic use</subject><issn>0960-3271</issn><issn>1477-0903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UE1LxDAQDaK46-rdkwQ8Vyff7VEWv0DwohcvJW0SydKma5oe9t-bdVcFwdMM8968mfcQOidwRYhS11BJYFQRChXhlWQHaE64UgVUwA7RfAsXW3yGTsZxBQCyEuQYzRiljJVQztHqzYcW62DwaDsb_NTjdugbH3TyQ8ApWp16GxJexyHZNlmDjdeNTXYsfDBTmwe5T76dOh2_hOzaG282ve6w0b1-t9gHHHU6RUdOd6M929cFer27fVk-FE_P94_Lm6eiZVKkgnOttBRCs5JzUjoOuQhrpKycEhyAcuGyKU6kI65phCkdKAVl66itqGILdLnTzS9_TPm3ejVMMeSTNeVUlFSWTGYW7FhtHMYxWlevo-913NQE6m249d9w88rFXnhqemt-Fr7TzIRiRxiz69-r_wp-Ak9DgPc</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Sahu, C</creator><creator>Dwivedi, DK</creator><creator>Jena, GB</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-9437-7252</orcidid></search><sort><creationdate>202009</creationdate><title>Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat</title><author>Sahu, C ; Dwivedi, DK ; Jena, GB</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-44a7a655a384418f404415ed669f75400245f327416f1fbb5d8f07708cf2e9273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Bioassays</topic><topic>Biochemical analysis</topic><topic>Body weight</topic><topic>Catalase</topic><topic>Catalase - analysis</topic><topic>Combined treatment</topic><topic>Comet assay</topic><topic>Cytology</topic><topic>Damage assessment</topic><topic>Damage detection</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (insulin dependent)</topic><topic>Diabetes Mellitus, Experimental - chemically induced</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Type 1 - chemically induced</topic><topic>Diabetes Mellitus, Type 1 - complications</topic><topic>DNA nucleotidylexotransferase</topic><topic>Epididymis - drug effects</topic><topic>Epididymis - pathology</topic><topic>Gene expression</topic><topic>Glutathione</topic><topic>Glutathione - analysis</topic><topic>Glutathione peroxidase</topic><topic>Histology</topic><topic>Hypogonadism</topic><topic>Immunohistochemistry</topic><topic>Impact damage</topic><topic>Infertility</topic><topic>Lipid Peroxidation</topic><topic>Male</topic><topic>Morphology</topic><topic>Organ weight</topic><topic>Pattern analysis</topic><topic>Peroxidase</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Selenium</topic><topic>Selenium - pharmacology</topic><topic>Selenium - therapeutic use</topic><topic>Serum proteins</topic><topic>Sperm</topic><topic>Sperm Count</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - pathology</topic><topic>Streptozocin</topic><topic>Testis - drug effects</topic><topic>Testis - pathology</topic><topic>Toxicity</topic><topic>Trace elements</topic><topic>Transmission electron microscopy</topic><topic>Water intake</topic><topic>Water intakes</topic><topic>Zinc</topic><topic>Zinc - pharmacology</topic><topic>Zinc - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahu, C</creatorcontrib><creatorcontrib>Dwivedi, DK</creatorcontrib><creatorcontrib>Jena, GB</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Environment Abstracts</collection><jtitle>Human & experimental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Sahu, C</au><au>Dwivedi, DK</au><au>Jena, GB</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat</atitle><jtitle>Human & experimental toxicology</jtitle><addtitle>Hum Exp Toxicol</addtitle><date>2020-09</date><risdate>2020</risdate><volume>39</volume><issue>9</issue><spage>1235</spage><epage>1256</epage><pages>1235-1256</pages><issn>0960-3271</issn><eissn>1477-0903</eissn><abstract>Diabetes increases the possibility of germ cell damage, hypogonadism, and male infertility. Diabetic condition negatively impacts zinc (Zn) and selenium (Se) levels in the body. Zn and Se are among the most important trace elements involved in the regulation of redox reaction, antioxidants enzymes activities, and DNA expression in a germ cell. The present study aimed to elucidate the combined effects of Zn and Se treatment on diabetes-induced germ cell damage in male Sprague Dawley rats. Type 1 diabetes was induced by the single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) were administered daily for 8 consecutive weeks. All the animals were provided with normal feed and water throughout the study. The effects on germ cell damage were evaluated by body weight, feed-water intake, organ weight, sperm count, motility, sperm head morphology, biochemical analysis, histology, immunohistochemistry, halo assay, germ cell comet assay, testes terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end-labeling (TUNEL) assay, sperm TUNEL assay, serum protein pattern analysis, and subcellular analysis using transmission electron microscopy. Further, the expressions of nuclear erythroid-derived related factor 2, catalase, glutathione peroxidase 4, and glutathione peroxidase 5 were carried out to ascertain the mechanism of protection. The present results demonstrated that 8 weeks combined treatment of Zn (3 mg/kg, i.p.) and Se (0.5 mg/kg, i.p.) reduced diabetes-induced germ cell damage. This study further highlighted that Zn and Se combination treatment might be a better strategy for the germ cell protection in diabetes and deserve further investigation.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>32233808</pmid><doi>10.1177/0960327120914963</doi><tpages>22</tpages><orcidid>https://orcid.org/0000-0001-9437-7252</orcidid></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 0960-3271 |
| ispartof | Human & experimental toxicology, 2020-09, Vol.39 (9), p.1235-1256 |
| issn | 0960-3271 1477-0903 |
| language | eng |
| recordid | cdi_proquest_journals_2425826836 |
| source | Sage Journals GOLD Open Access 2024 |
| subjects | Animals Antioxidants Bioassays Biochemical analysis Body weight Catalase Catalase - analysis Combined treatment Comet assay Cytology Damage assessment Damage detection Diabetes Diabetes mellitus Diabetes mellitus (insulin dependent) Diabetes Mellitus, Experimental - chemically induced Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Type 1 - chemically induced Diabetes Mellitus, Type 1 - complications DNA nucleotidylexotransferase Epididymis - drug effects Epididymis - pathology Gene expression Glutathione Glutathione - analysis Glutathione peroxidase Histology Hypogonadism Immunohistochemistry Impact damage Infertility Lipid Peroxidation Male Morphology Organ weight Pattern analysis Peroxidase Rats Rats, Sprague-Dawley Selenium Selenium - pharmacology Selenium - therapeutic use Serum proteins Sperm Sperm Count Sperm Motility - drug effects Spermatozoa - drug effects Spermatozoa - pathology Streptozocin Testis - drug effects Testis - pathology Toxicity Trace elements Transmission electron microscopy Water intake Water intakes Zinc Zinc - pharmacology Zinc - therapeutic use |
| title | Zinc and selenium combination treatment protected diabetes-induced testicular and epididymal damage in rat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T15%3A50%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_AFRWT&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Zinc%20and%20selenium%20combination%20treatment%20protected%20diabetes-induced%20testicular%20and%20epididymal%20damage%20in%20rat&rft.jtitle=Human%20&%20experimental%20toxicology&rft.au=Sahu,%20C&rft.date=2020-09&rft.volume=39&rft.issue=9&rft.spage=1235&rft.epage=1256&rft.pages=1235-1256&rft.issn=0960-3271&rft.eissn=1477-0903&rft_id=info:doi/10.1177/0960327120914963&rft_dat=%3Cproquest_AFRWT%3E2425826836%3C/proquest_AFRWT%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2425826836&rft_id=info:pmid/32233808&rft_sage_id=10.1177_0960327120914963&rfr_iscdi=true |