Biochanin-A elicits relaxation in coronary artery of goat through different mechanisms
Flavonoids have shown beneficial effects in various disease conditions as reported by various previous studies. Biochanin-A is a flavonoid present in various plants in nature. Present investigation was done to assess the vasorelaxant potential of biochanin-A on isolated coronary artery of goat and i...
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| Veröffentlicht in: | Research in veterinary science 2020-08, Vol.131, p.206-214 |
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| creator | Kumar, Tarun Sharma, Meemansha Rana, Abhinav Lingaraju, Madhu Cholenahalli Parida, Subhashree Kumar, Dinesh Singh, Thakur Uttam |
| description | Flavonoids have shown beneficial effects in various disease conditions as reported by various previous studies. Biochanin-A is a flavonoid present in various plants in nature. Present investigation was done to assess the vasorelaxant potential of biochanin-A on isolated coronary artery of goat and its possible mechanism of action. Vascular reactivity experiments were done on circumflex coronary artery of goats using the tension experiments. Goat coronary arterial rings were relaxed with biochanin-A in concentration (0.1–100 μM)-dependent manner. Endothelium had no effect on biochanin-A-induced relaxation. Maximum relaxation induced by biochanin-A was 116.54 ± 12.21% in endothelium-intact artery and it was not significantly different with maximal relaxation (108.22 ± 1.88%) of endothelium-denuded vessel. L-NAME (100 μM) did not show any effect on biochanin-A-induced relaxation. TEA (BKCa channel blocker), and BaCl2 (KIR blocker) had no effect on biochanin-A-induced relaxation. However, biochanin-A-induced maximal relaxation (71.72 ± 4.50%) was reduced significantly (P |
| doi_str_mv | 10.1016/j.rvsc.2020.05.003 |
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•Biochanin-A showed concentration-dependent relaxation in the isolated coronary artery of goat.•Relaxation response induced by the biochanin-A was mediated through the voltage gated potassium channels.•Cav1.2 channels were also involved in the biochanin-A-induced relaxation in the goat coronary artery.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2020.05.003</identifier><identifier>PMID: 32408231</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Attenuation ; Barium chloride ; Biochanin-A ; Blood vessels ; Calcium channels (voltage-gated) ; Calcium chloride ; Cardiovascular disease ; Cav1.2 channels ; Coronary artery ; Depolarization ; Endothelium ; Enzyme inhibitors ; Estrogen receptors ; Estrogens ; Flavonoids ; Goat ; Goats ; Hormone replacement therapy ; Kinases ; KV channels ; NG-Nitroarginine methyl ester ; Potassium ; Protein kinase A ; Receptors ; Rho-associated kinase ; Software ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2020-08, Vol.131, p.206-214</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>2020. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-6bccd890a7f8a0defffe89b8d77e7cb422c3b7cbbd302934a90de9b57d0a47c03</citedby><cites>FETCH-LOGICAL-c384t-6bccd890a7f8a0defffe89b8d77e7cb422c3b7cbbd302934a90de9b57d0a47c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0034528819302358$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32408231$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Tarun</creatorcontrib><creatorcontrib>Sharma, Meemansha</creatorcontrib><creatorcontrib>Rana, Abhinav</creatorcontrib><creatorcontrib>Lingaraju, Madhu Cholenahalli</creatorcontrib><creatorcontrib>Parida, Subhashree</creatorcontrib><creatorcontrib>Kumar, Dinesh</creatorcontrib><creatorcontrib>Singh, Thakur Uttam</creatorcontrib><title>Biochanin-A elicits relaxation in coronary artery of goat through different mechanisms</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Flavonoids have shown beneficial effects in various disease conditions as reported by various previous studies. Biochanin-A is a flavonoid present in various plants in nature. Present investigation was done to assess the vasorelaxant potential of biochanin-A on isolated coronary artery of goat and its possible mechanism of action. Vascular reactivity experiments were done on circumflex coronary artery of goats using the tension experiments. Goat coronary arterial rings were relaxed with biochanin-A in concentration (0.1–100 μM)-dependent manner. Endothelium had no effect on biochanin-A-induced relaxation. Maximum relaxation induced by biochanin-A was 116.54 ± 12.21% in endothelium-intact artery and it was not significantly different with maximal relaxation (108.22 ± 1.88%) of endothelium-denuded vessel. L-NAME (100 μM) did not show any effect on biochanin-A-induced relaxation. TEA (BKCa channel blocker), and BaCl2 (KIR blocker) had no effect on biochanin-A-induced relaxation. However, biochanin-A-induced maximal relaxation (71.72 ± 4.50%) was reduced significantly (P < .001) in the presence of 4-aminopyridine (KV channel blocker, 3 mM) in comparison with control (114.07 ± 4.33%). Glibenclaminde (KATP channel blocker), H89 (PKA inhibitor), ICI182780 (estrogen receptor antagonist) showed partial attenuation in the biochanin-A-induced relaxation. ODQ (sGC blocker) and HC067047 (TRPV4 channel blocker) had no effect on biochanin-A-induced relaxation. In K+-depolarized endothelium-denuded arterial rings, biochanin-A (30 μM) significantly (P < .05; P < .001) decreased CaCl2-induced contractions (0.02 ± 0.01 g vs. control 0.73 ± 0.30 g). Biochanin-A did not influence the fasudil (rho kinase inhibitor) and SNP (NO-donor)-induced relaxation in this vessel. Biochanin-A showed relaxation in goat coronary artery in endothelium-independent pathways and showed the partial involvement of KATP, protein kinase A and estrogen receptors and full involvement of Cav1.2 channels.
•Biochanin-A showed concentration-dependent relaxation in the isolated coronary artery of goat.•Relaxation response induced by the biochanin-A was mediated through the voltage gated potassium channels.•Cav1.2 channels were also involved in the biochanin-A-induced relaxation in the goat coronary artery.</description><subject>Attenuation</subject><subject>Barium chloride</subject><subject>Biochanin-A</subject><subject>Blood vessels</subject><subject>Calcium channels (voltage-gated)</subject><subject>Calcium chloride</subject><subject>Cardiovascular disease</subject><subject>Cav1.2 channels</subject><subject>Coronary artery</subject><subject>Depolarization</subject><subject>Endothelium</subject><subject>Enzyme inhibitors</subject><subject>Estrogen receptors</subject><subject>Estrogens</subject><subject>Flavonoids</subject><subject>Goat</subject><subject>Goats</subject><subject>Hormone replacement therapy</subject><subject>Kinases</subject><subject>KV channels</subject><subject>NG-Nitroarginine methyl ester</subject><subject>Potassium</subject><subject>Protein kinase A</subject><subject>Receptors</subject><subject>Rho-associated kinase</subject><subject>Software</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWj_-gAcJeN51kuxHFrxo8QsEL-o1ZJNZm9JuNElF_72pVY-eZhjeeYZ5CDlmUDJgzdm8DO_RlBw4lFCXAGKLTFgteMGbhm2TSZ5URc2l3CP7Mc4BoGKs3SV7glcguWAT8nzpvJnp0Y3FBcWFMy5FGnChP3RyfqRupMYHP-rwSXVImIsf6IvXiaZZ8KuXGbVuGDDgmOgSv1FxGQ_JzqAXEY9-6gF5ur56nN4W9w83d9OL-8IIWaWi6Y2xsgPdDlKDxSGTZNdL27bYmr7i3Ig-N70VwDtR6S6Hur5uLeiqNSAOyOmG-xr82wpjUnO_CmM-qXjF60byVoic4puUCT7GgIN6DW6ZX1IM1Fqlmqu1SrVWqaBWWVxeOvlBr_ol2r-VX3c5cL4JYH7w3WFQ0TgcDVoX0CRlvfuP_wXn4Iaj</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Kumar, Tarun</creator><creator>Sharma, Meemansha</creator><creator>Rana, Abhinav</creator><creator>Lingaraju, Madhu Cholenahalli</creator><creator>Parida, Subhashree</creator><creator>Kumar, Dinesh</creator><creator>Singh, Thakur Uttam</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20200801</creationdate><title>Biochanin-A elicits relaxation in coronary artery of goat through different mechanisms</title><author>Kumar, Tarun ; Sharma, Meemansha ; Rana, Abhinav ; Lingaraju, Madhu Cholenahalli ; Parida, Subhashree ; Kumar, Dinesh ; Singh, Thakur Uttam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-6bccd890a7f8a0defffe89b8d77e7cb422c3b7cbbd302934a90de9b57d0a47c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Attenuation</topic><topic>Barium chloride</topic><topic>Biochanin-A</topic><topic>Blood vessels</topic><topic>Calcium channels (voltage-gated)</topic><topic>Calcium chloride</topic><topic>Cardiovascular disease</topic><topic>Cav1.2 channels</topic><topic>Coronary artery</topic><topic>Depolarization</topic><topic>Endothelium</topic><topic>Enzyme inhibitors</topic><topic>Estrogen receptors</topic><topic>Estrogens</topic><topic>Flavonoids</topic><topic>Goat</topic><topic>Goats</topic><topic>Hormone replacement therapy</topic><topic>Kinases</topic><topic>KV channels</topic><topic>NG-Nitroarginine methyl ester</topic><topic>Potassium</topic><topic>Protein kinase A</topic><topic>Receptors</topic><topic>Rho-associated kinase</topic><topic>Software</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Tarun</creatorcontrib><creatorcontrib>Sharma, Meemansha</creatorcontrib><creatorcontrib>Rana, Abhinav</creatorcontrib><creatorcontrib>Lingaraju, Madhu Cholenahalli</creatorcontrib><creatorcontrib>Parida, Subhashree</creatorcontrib><creatorcontrib>Kumar, Dinesh</creatorcontrib><creatorcontrib>Singh, Thakur Uttam</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Tarun</au><au>Sharma, Meemansha</au><au>Rana, Abhinav</au><au>Lingaraju, Madhu Cholenahalli</au><au>Parida, Subhashree</au><au>Kumar, Dinesh</au><au>Singh, Thakur Uttam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochanin-A elicits relaxation in coronary artery of goat through different mechanisms</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>131</volume><spage>206</spage><epage>214</epage><pages>206-214</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Flavonoids have shown beneficial effects in various disease conditions as reported by various previous studies. Biochanin-A is a flavonoid present in various plants in nature. Present investigation was done to assess the vasorelaxant potential of biochanin-A on isolated coronary artery of goat and its possible mechanism of action. Vascular reactivity experiments were done on circumflex coronary artery of goats using the tension experiments. Goat coronary arterial rings were relaxed with biochanin-A in concentration (0.1–100 μM)-dependent manner. Endothelium had no effect on biochanin-A-induced relaxation. Maximum relaxation induced by biochanin-A was 116.54 ± 12.21% in endothelium-intact artery and it was not significantly different with maximal relaxation (108.22 ± 1.88%) of endothelium-denuded vessel. L-NAME (100 μM) did not show any effect on biochanin-A-induced relaxation. TEA (BKCa channel blocker), and BaCl2 (KIR blocker) had no effect on biochanin-A-induced relaxation. However, biochanin-A-induced maximal relaxation (71.72 ± 4.50%) was reduced significantly (P < .001) in the presence of 4-aminopyridine (KV channel blocker, 3 mM) in comparison with control (114.07 ± 4.33%). Glibenclaminde (KATP channel blocker), H89 (PKA inhibitor), ICI182780 (estrogen receptor antagonist) showed partial attenuation in the biochanin-A-induced relaxation. ODQ (sGC blocker) and HC067047 (TRPV4 channel blocker) had no effect on biochanin-A-induced relaxation. In K+-depolarized endothelium-denuded arterial rings, biochanin-A (30 μM) significantly (P < .05; P < .001) decreased CaCl2-induced contractions (0.02 ± 0.01 g vs. control 0.73 ± 0.30 g). Biochanin-A did not influence the fasudil (rho kinase inhibitor) and SNP (NO-donor)-induced relaxation in this vessel. Biochanin-A showed relaxation in goat coronary artery in endothelium-independent pathways and showed the partial involvement of KATP, protein kinase A and estrogen receptors and full involvement of Cav1.2 channels.
•Biochanin-A showed concentration-dependent relaxation in the isolated coronary artery of goat.•Relaxation response induced by the biochanin-A was mediated through the voltage gated potassium channels.•Cav1.2 channels were also involved in the biochanin-A-induced relaxation in the goat coronary artery.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32408231</pmid><doi>10.1016/j.rvsc.2020.05.003</doi><tpages>9</tpages></addata></record> |
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| subjects | Attenuation Barium chloride Biochanin-A Blood vessels Calcium channels (voltage-gated) Calcium chloride Cardiovascular disease Cav1.2 channels Coronary artery Depolarization Endothelium Enzyme inhibitors Estrogen receptors Estrogens Flavonoids Goat Goats Hormone replacement therapy Kinases KV channels NG-Nitroarginine methyl ester Potassium Protein kinase A Receptors Rho-associated kinase Software Veterinary medicine |
| title | Biochanin-A elicits relaxation in coronary artery of goat through different mechanisms |
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