Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2
An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2). Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization. HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) c...
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| Veröffentlicht in: | Journal of nanomaterials 2020, Vol.2020 (2020), p.1-6 |
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| container_title | Journal of nanomaterials |
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| creator | Yu, Yi Lu, Xuan-Yi Zhao, Zong-Ya Jiang, Wen-Shuai Wang, Chang Ren, Wu Yang, Fen Ren, Qiong-Qiong Chen, Jun |
| description | An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2). Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization. HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) coated carbon fiber ultramicroelectrodes (CFUMEs). The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode. Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2. The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2. The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM. Furthermore, the biosensor exhibited long-term stability and good reproducibility. Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples. |
| doi_str_mv | 10.1155/2020/9627697 |
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S.</contributor><creatorcontrib>Yu, Yi ; Lu, Xuan-Yi ; Zhao, Zong-Ya ; Jiang, Wen-Shuai ; Wang, Chang ; Ren, Wu ; Yang, Fen ; Ren, Qiong-Qiong ; Chen, Jun ; Chauhan, Bhanu P. S.</creatorcontrib><description>An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2). Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization. HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) coated carbon fiber ultramicroelectrodes (CFUMEs). The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode. Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2. The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2. The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM. Furthermore, the biosensor exhibited long-term stability and good reproducibility. Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples.</description><identifier>ISSN: 1687-4110</identifier><identifier>EISSN: 1687-4129</identifier><identifier>DOI: 10.1155/2020/9627697</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Biological activity ; Biosensors ; Carbon fibers ; Coimmobilization ; Electrical measurement ; Electrodes ; Electron transfer ; Enzymes ; Experiments ; Fluorescence ; Gold ; Hydrogen peroxide ; Multi wall carbon nanotubes ; Nanoclusters ; Nanomaterials ; Nanoparticles ; Peroxidase ; Sensors</subject><ispartof>Journal of nanomaterials, 2020, Vol.2020 (2020), p.1-6</ispartof><rights>Copyright © 2020 Qiong-Qiong Ren et al.</rights><rights>Copyright © 2020 Qiong-Qiong Ren et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-6636-606X ; 0000-0002-8023-9753</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><contributor>Chauhan, Bhanu P. 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The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode. Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2. The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2. The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM. Furthermore, the biosensor exhibited long-term stability and good reproducibility. Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples.</description><subject>Biological activity</subject><subject>Biosensors</subject><subject>Carbon fibers</subject><subject>Coimmobilization</subject><subject>Electrical measurement</subject><subject>Electrodes</subject><subject>Electron transfer</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Fluorescence</subject><subject>Gold</subject><subject>Hydrogen peroxide</subject><subject>Multi wall carbon nanotubes</subject><subject>Nanoclusters</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Peroxidase</subject><subject>Sensors</subject><issn>1687-4110</issn><issn>1687-4129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>BENPR</sourceid><recordid>eNpFkEFP3DAUhKOqSKXQW8_IEkcUsJ-d2DnubmEXibIc2nNkxzZ4lcSL7YjCf-G_4mURPb2n0Tcz0hTFT4LPCamqC8CAL5oaeN3wL8UhqQUvGYHm6-dP8Lfie4wbjFnVVHBYvM6GrQl-MCm4Ds2dj2aMPqC5jEYjP6KFd8Pglevdi0wuC96i31Of3JPs-4wsZFBZvZWjT5MyEclRo5UP0QSpXXxAdzn-n9M5r1z6Xr-TXT_FZMKur_PjZrqXKTtt7v1lkumSG-_RCtZwXBxY2Ufz4-MeFX-vLv8sVuXNenm9mN2UBgROpQXRcNExyRTXWqkOV5RiShTBjRGagLVMVUJLTigFwJZDwy1QiTWoumP0qDjd526Df5xMTO3GT2HMlS0wYILXFcOZOttTD27U8sm12-AGGZ5bgtvd_u1u__Zj_0yf7GmTGWPlf5oIUVOgb863g-8</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Yu, Yi</creator><creator>Lu, Xuan-Yi</creator><creator>Zhao, Zong-Ya</creator><creator>Jiang, Wen-Shuai</creator><creator>Wang, Chang</creator><creator>Ren, Wu</creator><creator>Yang, Fen</creator><creator>Ren, Qiong-Qiong</creator><creator>Chen, Jun</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-6636-606X</orcidid><orcidid>https://orcid.org/0000-0002-8023-9753</orcidid></search><sort><creationdate>2020</creationdate><title>Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2</title><author>Yu, Yi ; Lu, Xuan-Yi ; Zhao, Zong-Ya ; Jiang, Wen-Shuai ; Wang, Chang ; Ren, Wu ; Yang, Fen ; Ren, Qiong-Qiong ; Chen, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e280t-f28978c4a4b7ddbbc0533031b109e8d12ff4b58da7133220f7297f23a0d2b6c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biological activity</topic><topic>Biosensors</topic><topic>Carbon fibers</topic><topic>Coimmobilization</topic><topic>Electrical measurement</topic><topic>Electrodes</topic><topic>Electron transfer</topic><topic>Enzymes</topic><topic>Experiments</topic><topic>Fluorescence</topic><topic>Gold</topic><topic>Hydrogen peroxide</topic><topic>Multi wall carbon nanotubes</topic><topic>Nanoclusters</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Peroxidase</topic><topic>Sensors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Yi</creatorcontrib><creatorcontrib>Lu, Xuan-Yi</creatorcontrib><creatorcontrib>Zhao, Zong-Ya</creatorcontrib><creatorcontrib>Jiang, Wen-Shuai</creatorcontrib><creatorcontrib>Wang, Chang</creatorcontrib><creatorcontrib>Ren, Wu</creatorcontrib><creatorcontrib>Yang, Fen</creatorcontrib><creatorcontrib>Ren, Qiong-Qiong</creatorcontrib><creatorcontrib>Chen, Jun</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of nanomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Yi</au><au>Lu, Xuan-Yi</au><au>Zhao, Zong-Ya</au><au>Jiang, Wen-Shuai</au><au>Wang, Chang</au><au>Ren, Wu</au><au>Yang, Fen</au><au>Ren, Qiong-Qiong</au><au>Chen, Jun</au><au>Chauhan, Bhanu P. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2</atitle><jtitle>Journal of nanomaterials</jtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>1687-4110</issn><eissn>1687-4129</eissn><abstract>An enzyme-based amperometric biosensor was fabricated for detecting hydrogen peroxide (H2O2). Horseradish peroxidase (HRP) was modified using functionalized fluorescent gold nanoclusters (AuNCs) via biomineralization. HRP-AuNCs were successfully immobilized on multiwalled carbon nanotube- (MWCNT-) coated carbon fiber ultramicroelectrodes (CFUMEs). The AuNCs, which act as molecular electric wires, effectively promote the electron transfer between the enzyme active center and the electrode. Additionally, the HRP conjugated with the AuNCs retains its biological activity, which enables the catalytic reaction of H2O2. The HRP-AuNCs/MWCNTs/CFUMEs have been proven as excellent amperometric sensors for H2O2. The sensitivity of the H2O2 biosensor is 3.0×10−4 A/M, and the detection limit is estimated to be 443 nM. Furthermore, the biosensor exhibited long-term stability and good reproducibility. Moreover, the biosensor has been tested by determining the H2O2 concentration in calf serum samples.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><doi>10.1155/2020/9627697</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-6636-606X</orcidid><orcidid>https://orcid.org/0000-0002-8023-9753</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biological activity Biosensors Carbon fibers Coimmobilization Electrical measurement Electrodes Electron transfer Enzymes Experiments Fluorescence Gold Hydrogen peroxide Multi wall carbon nanotubes Nanoclusters Nanomaterials Nanoparticles Peroxidase Sensors |
| title | Amperometric Biosensor Based on Coimmobilization of Multiwalled Carbon Nanotubes and Horseradish Peroxidase-Gold Nanocluster Bioconjugates for Detecting H2O2 |
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