Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk
Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine mil...
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| Veröffentlicht in: | Food analytical methods 2020-08, Vol.13 (8), p.1539-1547 |
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| creator | Kergaravat, Silvina V. Nagel, Orlando G. Althaus, Rafael L. Hernández, Silvia R. |
| description | Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine milk. The assay is based on an indirect competitive method where the quinolone in the milk sample competed with the quinolone immobilized on magnetic beads for the anti-quinolone antibody. Then, an anti-IgG antibody–labeled peroxidase and their respective substrates were added and the fluorescent signal was indirectly related with the quinolone concentration. Recoveries of quinolones from 82 to 102% were obtained in skimmed, whole, and raw milk samples in concordance with the results obtained by a reference method. Seven quinolones were evaluated quantitatively by the method, obtaining limits of detection of 10 μg L
−1
for ciprofloxacin and marbofloxacin, 13 μg L
−1
for enrofloxacin and danofloxacin, 22 μg L
−1
for norfloxacin, 29 μg L
−1
for sarafloxacin, and 30 μg L
−1
for ofloxacin. For the screening detection of quinolones, ciprofloxacin was used as model with decision limit (CCα) and detection capability (CCβ) of 103 and 106 μg L
−1
, respectively. High cross-reactivity (> 100%) was obtained to all assayed quinolones while negligible cross-reactivity ( |
| doi_str_mv | 10.1007/s12161-020-01749-9 |
| format | Article |
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−1
for ciprofloxacin and marbofloxacin, 13 μg L
−1
for enrofloxacin and danofloxacin, 22 μg L
−1
for norfloxacin, 29 μg L
−1
for sarafloxacin, and 30 μg L
−1
for ofloxacin. For the screening detection of quinolones, ciprofloxacin was used as model with decision limit (CCα) and detection capability (CCβ) of 103 and 106 μg L
−1
, respectively. High cross-reactivity (> 100%) was obtained to all assayed quinolones while negligible cross-reactivity (< 0.1%) to other antibiotics (β-lactams, aminoglycosides, macrolides, tetracyclines, and sulfamides) was observed. The validation provided evidence that the method is suitable to be applied in routine analysis for the detection of quinolones in bovine milk.</description><identifier>ISSN: 1936-9751</identifier><identifier>EISSN: 1936-976X</identifier><identifier>DOI: 10.1007/s12161-020-01749-9</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aminoglycosides ; Analytical Chemistry ; Antibiotics ; Assaying ; Beads ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Ciprofloxacin ; Cow's milk ; Cross-reactivity ; Enrofloxacin ; Fluorescence ; Food Science ; IgG antibody ; Immunofluorescence ; Immunoglobulin G ; Microbiology ; Milk ; Norfloxacin ; Ofloxacin ; Peroxidase ; Quinolones ; Substrates ; Tetracyclines ; Veterinary medicine ; β-Lactam antibiotics</subject><ispartof>Food analytical methods, 2020-08, Vol.13 (8), p.1539-1547</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020. corrected publication May/2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020. corrected publication May/2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-ae1355cdd413c71a4e641424ed328261b1d9a5fae80c24842b26ca83be8cfdcd3</citedby><cites>FETCH-LOGICAL-c319t-ae1355cdd413c71a4e641424ed328261b1d9a5fae80c24842b26ca83be8cfdcd3</cites><orcidid>0000-0002-9765-6860</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12161-020-01749-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12161-020-01749-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Kergaravat, Silvina V.</creatorcontrib><creatorcontrib>Nagel, Orlando G.</creatorcontrib><creatorcontrib>Althaus, Rafael L.</creatorcontrib><creatorcontrib>Hernández, Silvia R.</creatorcontrib><title>Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk</title><title>Food analytical methods</title><addtitle>Food Anal. Methods</addtitle><description>Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine milk. The assay is based on an indirect competitive method where the quinolone in the milk sample competed with the quinolone immobilized on magnetic beads for the anti-quinolone antibody. Then, an anti-IgG antibody–labeled peroxidase and their respective substrates were added and the fluorescent signal was indirectly related with the quinolone concentration. Recoveries of quinolones from 82 to 102% were obtained in skimmed, whole, and raw milk samples in concordance with the results obtained by a reference method. Seven quinolones were evaluated quantitatively by the method, obtaining limits of detection of 10 μg L
−1
for ciprofloxacin and marbofloxacin, 13 μg L
−1
for enrofloxacin and danofloxacin, 22 μg L
−1
for norfloxacin, 29 μg L
−1
for sarafloxacin, and 30 μg L
−1
for ofloxacin. For the screening detection of quinolones, ciprofloxacin was used as model with decision limit (CCα) and detection capability (CCβ) of 103 and 106 μg L
−1
, respectively. High cross-reactivity (> 100%) was obtained to all assayed quinolones while negligible cross-reactivity (< 0.1%) to other antibiotics (β-lactams, aminoglycosides, macrolides, tetracyclines, and sulfamides) was observed. The validation provided evidence that the method is suitable to be applied in routine analysis for the detection of quinolones in bovine milk.</description><subject>Aminoglycosides</subject><subject>Analytical Chemistry</subject><subject>Antibiotics</subject><subject>Assaying</subject><subject>Beads</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Ciprofloxacin</subject><subject>Cow's milk</subject><subject>Cross-reactivity</subject><subject>Enrofloxacin</subject><subject>Fluorescence</subject><subject>Food Science</subject><subject>IgG antibody</subject><subject>Immunofluorescence</subject><subject>Immunoglobulin G</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Norfloxacin</subject><subject>Ofloxacin</subject><subject>Peroxidase</subject><subject>Quinolones</subject><subject>Substrates</subject><subject>Tetracyclines</subject><subject>Veterinary medicine</subject><subject>β-Lactam antibiotics</subject><issn>1936-9751</issn><issn>1936-976X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kNFKwzAUhoMoOKcv4FXB62hOkqbt5ZxuDjZEUPAuZOnp6OySmbTC3t5qRe-8OofD9_8HPkIugV0DY9lNBA4KKOOMMshkQYsjMoJCKFpk6vX4d0_hlJzFuGVMMQl8ROYrs3HY-mSx23XOV03nA0aLzmIyidEcksqH5KmrnW-8w-QOW7Rt7V1Su-TWf9T9bVU3b-fkpDJNxIufOSYvs_vn6QNdPs4X08mSWgFFSw2CSFNblhKEzcBIVBIkl1gKnnMFaygLk1YGc2a5zCVfc2VNLtaY26q0pRiTq6F3H_x7h7HVW98F17_UXHKRqpQp1VN8oGzwMQas9D7UOxMOGpj-EqYHYboXpr-F6aIPiSEUe9htMPxV_5P6BOtvbjA</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Kergaravat, Silvina V.</creator><creator>Nagel, Orlando G.</creator><creator>Althaus, Rafael L.</creator><creator>Hernández, Silvia R.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-9765-6860</orcidid></search><sort><creationdate>20200801</creationdate><title>Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk</title><author>Kergaravat, Silvina V. ; Nagel, Orlando G. ; Althaus, Rafael L. ; Hernández, Silvia R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-ae1355cdd413c71a4e641424ed328261b1d9a5fae80c24842b26ca83be8cfdcd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aminoglycosides</topic><topic>Analytical Chemistry</topic><topic>Antibiotics</topic><topic>Assaying</topic><topic>Beads</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Ciprofloxacin</topic><topic>Cow's milk</topic><topic>Cross-reactivity</topic><topic>Enrofloxacin</topic><topic>Fluorescence</topic><topic>Food Science</topic><topic>IgG antibody</topic><topic>Immunofluorescence</topic><topic>Immunoglobulin G</topic><topic>Microbiology</topic><topic>Milk</topic><topic>Norfloxacin</topic><topic>Ofloxacin</topic><topic>Peroxidase</topic><topic>Quinolones</topic><topic>Substrates</topic><topic>Tetracyclines</topic><topic>Veterinary medicine</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kergaravat, Silvina V.</creatorcontrib><creatorcontrib>Nagel, Orlando G.</creatorcontrib><creatorcontrib>Althaus, Rafael L.</creatorcontrib><creatorcontrib>Hernández, Silvia R.</creatorcontrib><collection>CrossRef</collection><jtitle>Food analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kergaravat, Silvina V.</au><au>Nagel, Orlando G.</au><au>Althaus, Rafael L.</au><au>Hernández, Silvia R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk</atitle><jtitle>Food analytical methods</jtitle><stitle>Food Anal. Methods</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>13</volume><issue>8</issue><spage>1539</spage><epage>1547</epage><pages>1539-1547</pages><issn>1936-9751</issn><eissn>1936-976X</eissn><abstract>Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine milk. The assay is based on an indirect competitive method where the quinolone in the milk sample competed with the quinolone immobilized on magnetic beads for the anti-quinolone antibody. Then, an anti-IgG antibody–labeled peroxidase and their respective substrates were added and the fluorescent signal was indirectly related with the quinolone concentration. Recoveries of quinolones from 82 to 102% were obtained in skimmed, whole, and raw milk samples in concordance with the results obtained by a reference method. Seven quinolones were evaluated quantitatively by the method, obtaining limits of detection of 10 μg L
−1
for ciprofloxacin and marbofloxacin, 13 μg L
−1
for enrofloxacin and danofloxacin, 22 μg L
−1
for norfloxacin, 29 μg L
−1
for sarafloxacin, and 30 μg L
−1
for ofloxacin. For the screening detection of quinolones, ciprofloxacin was used as model with decision limit (CCα) and detection capability (CCβ) of 103 and 106 μg L
−1
, respectively. High cross-reactivity (> 100%) was obtained to all assayed quinolones while negligible cross-reactivity (< 0.1%) to other antibiotics (β-lactams, aminoglycosides, macrolides, tetracyclines, and sulfamides) was observed. The validation provided evidence that the method is suitable to be applied in routine analysis for the detection of quinolones in bovine milk.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12161-020-01749-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9765-6860</orcidid></addata></record> |
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| subjects | Aminoglycosides Analytical Chemistry Antibiotics Assaying Beads Chemistry Chemistry and Materials Science Chemistry/Food Science Ciprofloxacin Cow's milk Cross-reactivity Enrofloxacin Fluorescence Food Science IgG antibody Immunofluorescence Immunoglobulin G Microbiology Milk Norfloxacin Ofloxacin Peroxidase Quinolones Substrates Tetracyclines Veterinary medicine β-Lactam antibiotics |
| title | Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk |
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