HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis
Key message Overexpression of HbWRKY40 induces ROS burst in tobacco and increases disease resistance in Arabidopsis; RNA-seq and ChIP assays revealed the regulatory network of HbWRKY40 in plant defense. WRKY, a family of plant transcription factors, are involved in the regulation of numerous biologi...
Gespeichert in:
| Veröffentlicht in: | Plant cell reports 2020-08, Vol.39 (8), p.1095-1107 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1107 |
|---|---|
| container_issue | 8 |
| container_start_page | 1095 |
| container_title | Plant cell reports |
| container_volume | 39 |
| creator | Yang, Jie Wang, Qiannan Luo, Hongli He, Chaozu An, Bang |
| description | Key message
Overexpression of HbWRKY40 induces ROS burst in tobacco and increases disease resistance in
Arabidopsis;
RNA-seq and ChIP assays revealed the regulatory network of HbWRKY40 in plant defense.
WRKY, a family of plant transcription factors, are involved in the regulation of numerous biological processes. In rubber tree
Hevea brasiliensis
, the roles of WRKYs remain poorly understood. In the present study, a total of 111 genes encoding putative HbWRKY proteins were identified in the
H. brasiliensis
genome. Among these genes,
HbWRKY40
transcripts were significantly induced by
Colletotrichum gloeosporioides
and salicylic acid. To assess its roles in plant defense, HbWRKY40 was over-expressed in
Nicotiana benthamiana
and
Arabidopsis thaliana
. The results showed that HbWRKY40 significantly induced reactive oxygen species burst in
N. benthamiana
and increased resistance of
Arabidopsis
against
Botrytis cinerea
. Transient expression in mesophyll cell protoplasts of
H. brasiliensis
showed that HbWRKY40 localizes at nuclei. In addition, transcripts of 145 genes were significantly up-regulated and 6 genes were down-regulated in the protoplasts over-expressing HbWRKY40 based on the RNA-seq analysis. Among these potential downstream targets, 12 genes contain potential WRKY-binding sites at the promoter regions. Further analysis through chromatin immunoprecipitation revealed that 10 of these 12 genes were the downstream targets of HbWRKY40. Taken together, our findings indicate that HbWRKY40 plays an important role in the disease resistance by regulating defense-associated genes in
H. brasiliensis. |
| doi_str_mv | 10.1007/s00299-020-02551-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2423342404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2423342404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-73eb6c544ef5d6e8663b8276ebf980b1b5a11b10f452f903e41368bf0ce7277c3</originalsourceid><addsrcrecordid>eNp9kF1LwzAUhoMobk7_gBcS8Lp68tFmvRRRJw4EUdSrkHSnW0fX1qSV7d8b16l3XoQDeZ_zHngIOWVwwQDUpQfgaRoBh_DimEXrPTJkUvCIg3jbJ0NQnEVKMTkgR94vAUKokkMyEFykaaLEkOiJfX16eJdAm9JsPDUVLVZN7VpTtdTVJdKiou0CqcN5V5q2qCta57Qx7aKeYxW-feEDnG3BCX6iodYZX5QFViE6Jge5KT2e7OaIvNzePF9Pounj3f311TTKhIrbSAm0SRZLiXk8S3CcJMKOuUrQ5ukYLLOxYcwyyGXM8xQESiaSsc0hQ8WVysSInPe9jas_OvStXtadq8JJzSUXQnIJMlC8pzJXe-8w140rVsZtNAP97VT3TnVwqrdO9Tosne2qO7vC2e_Kj8QAiB7wIarm6P5u_1P7BdZKgig</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2423342404</pqid></control><display><type>article</type><title>HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Yang, Jie ; Wang, Qiannan ; Luo, Hongli ; He, Chaozu ; An, Bang</creator><creatorcontrib>Yang, Jie ; Wang, Qiannan ; Luo, Hongli ; He, Chaozu ; An, Bang</creatorcontrib><description>Key message
Overexpression of HbWRKY40 induces ROS burst in tobacco and increases disease resistance in
Arabidopsis;
RNA-seq and ChIP assays revealed the regulatory network of HbWRKY40 in plant defense.
WRKY, a family of plant transcription factors, are involved in the regulation of numerous biological processes. In rubber tree
Hevea brasiliensis
, the roles of WRKYs remain poorly understood. In the present study, a total of 111 genes encoding putative HbWRKY proteins were identified in the
H. brasiliensis
genome. Among these genes,
HbWRKY40
transcripts were significantly induced by
Colletotrichum gloeosporioides
and salicylic acid. To assess its roles in plant defense, HbWRKY40 was over-expressed in
Nicotiana benthamiana
and
Arabidopsis thaliana
. The results showed that HbWRKY40 significantly induced reactive oxygen species burst in
N. benthamiana
and increased resistance of
Arabidopsis
against
Botrytis cinerea
. Transient expression in mesophyll cell protoplasts of
H. brasiliensis
showed that HbWRKY40 localizes at nuclei. In addition, transcripts of 145 genes were significantly up-regulated and 6 genes were down-regulated in the protoplasts over-expressing HbWRKY40 based on the RNA-seq analysis. Among these potential downstream targets, 12 genes contain potential WRKY-binding sites at the promoter regions. Further analysis through chromatin immunoprecipitation revealed that 10 of these 12 genes were the downstream targets of HbWRKY40. Taken together, our findings indicate that HbWRKY40 plays an important role in the disease resistance by regulating defense-associated genes in
H. brasiliensis.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-020-02551-x</identifier><identifier>PMID: 32399673</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Binding sites ; Biological activity ; Biomedical and Life Sciences ; Biotechnology ; Botrytis - drug effects ; Botrytis - physiology ; Botrytis cinerea ; Cell Biology ; Chromatin ; Colletotrichum - drug effects ; Colletotrichum - physiology ; Colletotrichum gloeosporioides ; Disease resistance ; Disease Resistance - genetics ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Genes ; Genes, Plant ; Genomes ; Hevea - drug effects ; Hevea - genetics ; Hevea - metabolism ; Hevea - microbiology ; Hevea brasiliensis ; Hydrogen Peroxide - metabolism ; Immunoprecipitation ; Life Sciences ; Mesophyll ; Nicotiana - genetics ; Original Article ; Phylogeny ; Plant Biochemistry ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Growth Regulators - pharmacology ; Plant Leaves - drug effects ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Protoplasts ; Protoplasts - drug effects ; Protoplasts - metabolism ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Ribonucleic acid ; RNA ; Rubber trees ; Salicylic acid ; Subcellular Fractions - metabolism ; Superoxides - metabolism ; Tobacco ; Transcription factors</subject><ispartof>Plant cell reports, 2020-08, Vol.39 (8), p.1095-1107</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-73eb6c544ef5d6e8663b8276ebf980b1b5a11b10f452f903e41368bf0ce7277c3</citedby><cites>FETCH-LOGICAL-c375t-73eb6c544ef5d6e8663b8276ebf980b1b5a11b10f452f903e41368bf0ce7277c3</cites><orcidid>0000-0003-4200-5777</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-020-02551-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-020-02551-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32399673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Jie</creatorcontrib><creatorcontrib>Wang, Qiannan</creatorcontrib><creatorcontrib>Luo, Hongli</creatorcontrib><creatorcontrib>He, Chaozu</creatorcontrib><creatorcontrib>An, Bang</creatorcontrib><title>HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
Overexpression of HbWRKY40 induces ROS burst in tobacco and increases disease resistance in
Arabidopsis;
RNA-seq and ChIP assays revealed the regulatory network of HbWRKY40 in plant defense.
WRKY, a family of plant transcription factors, are involved in the regulation of numerous biological processes. In rubber tree
Hevea brasiliensis
, the roles of WRKYs remain poorly understood. In the present study, a total of 111 genes encoding putative HbWRKY proteins were identified in the
H. brasiliensis
genome. Among these genes,
HbWRKY40
transcripts were significantly induced by
Colletotrichum gloeosporioides
and salicylic acid. To assess its roles in plant defense, HbWRKY40 was over-expressed in
Nicotiana benthamiana
and
Arabidopsis thaliana
. The results showed that HbWRKY40 significantly induced reactive oxygen species burst in
N. benthamiana
and increased resistance of
Arabidopsis
against
Botrytis cinerea
. Transient expression in mesophyll cell protoplasts of
H. brasiliensis
showed that HbWRKY40 localizes at nuclei. In addition, transcripts of 145 genes were significantly up-regulated and 6 genes were down-regulated in the protoplasts over-expressing HbWRKY40 based on the RNA-seq analysis. Among these potential downstream targets, 12 genes contain potential WRKY-binding sites at the promoter regions. Further analysis through chromatin immunoprecipitation revealed that 10 of these 12 genes were the downstream targets of HbWRKY40. Taken together, our findings indicate that HbWRKY40 plays an important role in the disease resistance by regulating defense-associated genes in
H. brasiliensis.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Binding sites</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Botrytis - drug effects</subject><subject>Botrytis - physiology</subject><subject>Botrytis cinerea</subject><subject>Cell Biology</subject><subject>Chromatin</subject><subject>Colletotrichum - drug effects</subject><subject>Colletotrichum - physiology</subject><subject>Colletotrichum gloeosporioides</subject><subject>Disease resistance</subject><subject>Disease Resistance - genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genomes</subject><subject>Hevea - drug effects</subject><subject>Hevea - genetics</subject><subject>Hevea - metabolism</subject><subject>Hevea - microbiology</subject><subject>Hevea brasiliensis</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Immunoprecipitation</subject><subject>Life Sciences</subject><subject>Mesophyll</subject><subject>Nicotiana - genetics</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Plant Biochemistry</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protoplasts</subject><subject>Protoplasts - drug effects</subject><subject>Protoplasts - metabolism</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Rubber trees</subject><subject>Salicylic acid</subject><subject>Subcellular Fractions - metabolism</subject><subject>Superoxides - metabolism</subject><subject>Tobacco</subject><subject>Transcription factors</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kF1LwzAUhoMobk7_gBcS8Lp68tFmvRRRJw4EUdSrkHSnW0fX1qSV7d8b16l3XoQDeZ_zHngIOWVwwQDUpQfgaRoBh_DimEXrPTJkUvCIg3jbJ0NQnEVKMTkgR94vAUKokkMyEFykaaLEkOiJfX16eJdAm9JsPDUVLVZN7VpTtdTVJdKiou0CqcN5V5q2qCta57Qx7aKeYxW-feEDnG3BCX6iodYZX5QFViE6Jge5KT2e7OaIvNzePF9Pounj3f311TTKhIrbSAm0SRZLiXk8S3CcJMKOuUrQ5ukYLLOxYcwyyGXM8xQESiaSsc0hQ8WVysSInPe9jas_OvStXtadq8JJzSUXQnIJMlC8pzJXe-8w140rVsZtNAP97VT3TnVwqrdO9Tosne2qO7vC2e_Kj8QAiB7wIarm6P5u_1P7BdZKgig</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Yang, Jie</creator><creator>Wang, Qiannan</creator><creator>Luo, Hongli</creator><creator>He, Chaozu</creator><creator>An, Bang</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-4200-5777</orcidid></search><sort><creationdate>20200801</creationdate><title>HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis</title><author>Yang, Jie ; Wang, Qiannan ; Luo, Hongli ; He, Chaozu ; An, Bang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-73eb6c544ef5d6e8663b8276ebf980b1b5a11b10f452f903e41368bf0ce7277c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Binding sites</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Botrytis - drug effects</topic><topic>Botrytis - physiology</topic><topic>Botrytis cinerea</topic><topic>Cell Biology</topic><topic>Chromatin</topic><topic>Colletotrichum - drug effects</topic><topic>Colletotrichum - physiology</topic><topic>Colletotrichum gloeosporioides</topic><topic>Disease resistance</topic><topic>Disease Resistance - genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genomes</topic><topic>Hevea - drug effects</topic><topic>Hevea - genetics</topic><topic>Hevea - metabolism</topic><topic>Hevea - microbiology</topic><topic>Hevea brasiliensis</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Immunoprecipitation</topic><topic>Life Sciences</topic><topic>Mesophyll</topic><topic>Nicotiana - genetics</topic><topic>Original Article</topic><topic>Phylogeny</topic><topic>Plant Biochemistry</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protoplasts</topic><topic>Protoplasts - drug effects</topic><topic>Protoplasts - metabolism</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Rubber trees</topic><topic>Salicylic acid</topic><topic>Subcellular Fractions - metabolism</topic><topic>Superoxides - metabolism</topic><topic>Tobacco</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Jie</creatorcontrib><creatorcontrib>Wang, Qiannan</creatorcontrib><creatorcontrib>Luo, Hongli</creatorcontrib><creatorcontrib>He, Chaozu</creatorcontrib><creatorcontrib>An, Bang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Jie</au><au>Wang, Qiannan</au><au>Luo, Hongli</au><au>He, Chaozu</au><au>An, Bang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>39</volume><issue>8</issue><spage>1095</spage><epage>1107</epage><pages>1095-1107</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
Overexpression of HbWRKY40 induces ROS burst in tobacco and increases disease resistance in
Arabidopsis;
RNA-seq and ChIP assays revealed the regulatory network of HbWRKY40 in plant defense.
WRKY, a family of plant transcription factors, are involved in the regulation of numerous biological processes. In rubber tree
Hevea brasiliensis
, the roles of WRKYs remain poorly understood. In the present study, a total of 111 genes encoding putative HbWRKY proteins were identified in the
H. brasiliensis
genome. Among these genes,
HbWRKY40
transcripts were significantly induced by
Colletotrichum gloeosporioides
and salicylic acid. To assess its roles in plant defense, HbWRKY40 was over-expressed in
Nicotiana benthamiana
and
Arabidopsis thaliana
. The results showed that HbWRKY40 significantly induced reactive oxygen species burst in
N. benthamiana
and increased resistance of
Arabidopsis
against
Botrytis cinerea
. Transient expression in mesophyll cell protoplasts of
H. brasiliensis
showed that HbWRKY40 localizes at nuclei. In addition, transcripts of 145 genes were significantly up-regulated and 6 genes were down-regulated in the protoplasts over-expressing HbWRKY40 based on the RNA-seq analysis. Among these potential downstream targets, 12 genes contain potential WRKY-binding sites at the promoter regions. Further analysis through chromatin immunoprecipitation revealed that 10 of these 12 genes were the downstream targets of HbWRKY40. Taken together, our findings indicate that HbWRKY40 plays an important role in the disease resistance by regulating defense-associated genes in
H. brasiliensis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32399673</pmid><doi>10.1007/s00299-020-02551-x</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-4200-5777</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2020-08, Vol.39 (8), p.1095-1107 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_journals_2423342404 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Arabidopsis Arabidopsis - genetics Binding sites Biological activity Biomedical and Life Sciences Biotechnology Botrytis - drug effects Botrytis - physiology Botrytis cinerea Cell Biology Chromatin Colletotrichum - drug effects Colletotrichum - physiology Colletotrichum gloeosporioides Disease resistance Disease Resistance - genetics Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Genes Genes, Plant Genomes Hevea - drug effects Hevea - genetics Hevea - metabolism Hevea - microbiology Hevea brasiliensis Hydrogen Peroxide - metabolism Immunoprecipitation Life Sciences Mesophyll Nicotiana - genetics Original Article Phylogeny Plant Biochemistry Plant Diseases - genetics Plant Diseases - microbiology Plant Growth Regulators - pharmacology Plant Leaves - drug effects Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified Promoter Regions, Genetic - genetics Protoplasts Protoplasts - drug effects Protoplasts - metabolism Reactive oxygen species Reactive Oxygen Species - metabolism Ribonucleic acid RNA Rubber trees Salicylic acid Subcellular Fractions - metabolism Superoxides - metabolism Tobacco Transcription factors |
| title | HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A00%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HbWRKY40%20plays%20an%20important%20role%20in%20the%20regulation%20of%20pathogen%20resistance%20in%20Hevea%20brasiliensis&rft.jtitle=Plant%20cell%20reports&rft.au=Yang,%20Jie&rft.date=2020-08-01&rft.volume=39&rft.issue=8&rft.spage=1095&rft.epage=1107&rft.pages=1095-1107&rft.issn=0721-7714&rft.eissn=1432-203X&rft_id=info:doi/10.1007/s00299-020-02551-x&rft_dat=%3Cproquest_cross%3E2423342404%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2423342404&rft_id=info:pmid/32399673&rfr_iscdi=true |