Complete Genome Sequence of Kosakonia radicincitans GXGL-4A, a Nitrogen-Fixing Bacterium with Capability to Degrade TEX
Kosakonia radicincitans GXGL-4A, a free-living nitrogen-fixing (NF) bacterial strain isolated from maize ( Zea mays L.) roots was found to have ability to degrade aromatic hydrocarbons. In this study, we describe the main morphological characteristics of bacterium, aromatic hydrocarbon-degrading cap...
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description | Kosakonia radicincitans
GXGL-4A, a free-living nitrogen-fixing (NF) bacterial strain isolated from maize (
Zea mays
L.) roots was found to have ability to degrade aromatic hydrocarbons. In this study, we describe the main morphological characteristics of bacterium, aromatic hydrocarbon-degrading capability, and the complete genome of
K
.
radicincitans
GXGL-4A. The genome is consisted of only one 5,687,681 bp linear chromosome with a G + C content of 53.96%. The strain has two genetically distinct nitrogenase systems, one based on molybdenum (Mo) similar to nitrogenase isolated from a wide range of nitrogen-fixing organisms, and the other contains iron (Fe). The differences in transcriptional level of several important nitrogen fixation (
nif
) genes between LB (nitrogen-rich, NR) and A15 nitrogen-free (nitrogen-limited, NL) culture conditions were detected using Real-time Quantitative Reverse Transcription PCR (qRT-PCR). The bacterial cells of GXGL-4A can grow well in LB liquid medium containing 1% toluene, ethylbenzene or xylene, suggesting a good resistance to the tested aromatic hydrocarbons. The results of GC–MS analysis showed that
K
.
radicincitans
GXGL-4A has a good capability to degrade toluene, ethylbenzene, and xylene (TEX). Completion of the genome sequencing will no doubt contribute to the deep exploration and comprehensive utilization of this NF bacterium in sustainable agriculture and bioremediation of aromatic pollutants. |
doi_str_mv | 10.1007/s00284-020-01942-3 |
format | Article |
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GXGL-4A, a free-living nitrogen-fixing (NF) bacterial strain isolated from maize (
Zea mays
L.) roots was found to have ability to degrade aromatic hydrocarbons. In this study, we describe the main morphological characteristics of bacterium, aromatic hydrocarbon-degrading capability, and the complete genome of
K
.
radicincitans
GXGL-4A. The genome is consisted of only one 5,687,681 bp linear chromosome with a G + C content of 53.96%. The strain has two genetically distinct nitrogenase systems, one based on molybdenum (Mo) similar to nitrogenase isolated from a wide range of nitrogen-fixing organisms, and the other contains iron (Fe). The differences in transcriptional level of several important nitrogen fixation (
nif
) genes between LB (nitrogen-rich, NR) and A15 nitrogen-free (nitrogen-limited, NL) culture conditions were detected using Real-time Quantitative Reverse Transcription PCR (qRT-PCR). The bacterial cells of GXGL-4A can grow well in LB liquid medium containing 1% toluene, ethylbenzene or xylene, suggesting a good resistance to the tested aromatic hydrocarbons. The results of GC–MS analysis showed that
K
.
radicincitans
GXGL-4A has a good capability to degrade toluene, ethylbenzene, and xylene (TEX). Completion of the genome sequencing will no doubt contribute to the deep exploration and comprehensive utilization of this NF bacterium in sustainable agriculture and bioremediation of aromatic pollutants.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-020-01942-3</identifier><identifier>PMID: 32170407</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aromatic hydrocarbons ; Bacteria ; Base Composition ; Benzene Derivatives - metabolism ; Biodegradation, Environmental ; Biomedical and Life Sciences ; Bioremediation ; Biotechnology ; Cell culture ; China ; Chromosomes ; Culture Media - chemistry ; Degradation ; Enterobacteriaceae - genetics ; Enterobacteriaceae - metabolism ; Ethylbenzene ; Gene sequencing ; Genome, Bacterial ; Genomes ; Hydrocarbon-degrading bacteria ; Hydrocarbons ; Hydrocarbons, Aromatic - metabolism ; Iron ; Kosakonia radicincitans ; Life Sciences ; Microbiology ; Molybdenum ; Nitrogen ; Nitrogen Fixation ; Nitrogenase ; Nitrogenase - metabolism ; Nitrogenation ; Nucleotide sequence ; Physical characteristics ; Plant Roots - microbiology ; Pollutants ; Reverse transcription ; Sequence Analysis, DNA ; Sustainable agriculture ; Toluene ; Toluene - metabolism ; Whole Genome Sequencing ; Xylene ; Xylenes - metabolism ; Zea mays ; Zea mays - microbiology</subject><ispartof>Current microbiology, 2020-08, Vol.77 (8), p.1848-1857</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a8fb99c6b18838dec78e05fed83160ff0a45a47b3aad373a2eedff038670dc0d3</citedby><cites>FETCH-LOGICAL-c375t-a8fb99c6b18838dec78e05fed83160ff0a45a47b3aad373a2eedff038670dc0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-020-01942-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-020-01942-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32170407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yunpeng</creatorcontrib><creatorcontrib>Huang, Zhibo</creatorcontrib><creatorcontrib>Li, Jiaoyong</creatorcontrib><creatorcontrib>Su, Guoxun</creatorcontrib><creatorcontrib>Feng, Baoyun</creatorcontrib><title>Complete Genome Sequence of Kosakonia radicincitans GXGL-4A, a Nitrogen-Fixing Bacterium with Capability to Degrade TEX</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Kosakonia radicincitans
GXGL-4A, a free-living nitrogen-fixing (NF) bacterial strain isolated from maize (
Zea mays
L.) roots was found to have ability to degrade aromatic hydrocarbons. In this study, we describe the main morphological characteristics of bacterium, aromatic hydrocarbon-degrading capability, and the complete genome of
K
.
radicincitans
GXGL-4A. The genome is consisted of only one 5,687,681 bp linear chromosome with a G + C content of 53.96%. The strain has two genetically distinct nitrogenase systems, one based on molybdenum (Mo) similar to nitrogenase isolated from a wide range of nitrogen-fixing organisms, and the other contains iron (Fe). The differences in transcriptional level of several important nitrogen fixation (
nif
) genes between LB (nitrogen-rich, NR) and A15 nitrogen-free (nitrogen-limited, NL) culture conditions were detected using Real-time Quantitative Reverse Transcription PCR (qRT-PCR). The bacterial cells of GXGL-4A can grow well in LB liquid medium containing 1% toluene, ethylbenzene or xylene, suggesting a good resistance to the tested aromatic hydrocarbons. The results of GC–MS analysis showed that
K
.
radicincitans
GXGL-4A has a good capability to degrade toluene, ethylbenzene, and xylene (TEX). Completion of the genome sequencing will no doubt contribute to the deep exploration and comprehensive utilization of this NF bacterium in sustainable agriculture and bioremediation of aromatic pollutants.</description><subject>Aromatic hydrocarbons</subject><subject>Bacteria</subject><subject>Base Composition</subject><subject>Benzene Derivatives - metabolism</subject><subject>Biodegradation, Environmental</subject><subject>Biomedical and Life Sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>China</subject><subject>Chromosomes</subject><subject>Culture Media - chemistry</subject><subject>Degradation</subject><subject>Enterobacteriaceae - genetics</subject><subject>Enterobacteriaceae - metabolism</subject><subject>Ethylbenzene</subject><subject>Gene sequencing</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Hydrocarbon-degrading bacteria</subject><subject>Hydrocarbons</subject><subject>Hydrocarbons, Aromatic - metabolism</subject><subject>Iron</subject><subject>Kosakonia radicincitans</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molybdenum</subject><subject>Nitrogen</subject><subject>Nitrogen Fixation</subject><subject>Nitrogenase</subject><subject>Nitrogenase - metabolism</subject><subject>Nitrogenation</subject><subject>Nucleotide sequence</subject><subject>Physical characteristics</subject><subject>Plant Roots - microbiology</subject><subject>Pollutants</subject><subject>Reverse transcription</subject><subject>Sequence Analysis, DNA</subject><subject>Sustainable agriculture</subject><subject>Toluene</subject><subject>Toluene - metabolism</subject><subject>Whole Genome Sequencing</subject><subject>Xylene</subject><subject>Xylenes - metabolism</subject><subject>Zea mays</subject><subject>Zea mays - microbiology</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kE1vEzEQhi1ERUPhD3BAlrhiOv5IbB9LaAMiKgeK1Jvl3Z0NLlk7tR2V_nsMKXDraaSZ90PzEPKKwzsOoE8LgDCKgQAG3CrB5BMy40oKBtbyp2QGUklmFnN-TJ6XcgPAhQX-jBxLwTUo0DNyt0zTbosV6QpjmpB-xds9xh5pGunnVPyPFIOn2Q-hD7EP1cdCV9erNVNnb6mnl6HmtMHILsLPEDf0ve8r5rCf6F2o3-nS73wXtqHe05roB9y0IKRX59cvyNHotwVfPswT8u3i_Gr5ka2_rD4tz9asl3pemTdjZ22_6Lgx0gzYa4MwH3Ewki9gHMGruVe6k94PUksvEIe2lWahYehhkCfkzSF3l1N7rFR3k_Y5tkonFLdaW2lNU4mDqs-plIyj2-Uw-XzvOLjfrN2BtWus3R_WTjbT64fofTfh8M_yF24TyIOgtFPcYP7f_UjsLzuRidg</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Chen, Yunpeng</creator><creator>Huang, Zhibo</creator><creator>Li, Jiaoyong</creator><creator>Su, Guoxun</creator><creator>Feng, Baoyun</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20200801</creationdate><title>Complete Genome Sequence of Kosakonia radicincitans GXGL-4A, a Nitrogen-Fixing Bacterium with Capability to Degrade TEX</title><author>Chen, Yunpeng ; Huang, Zhibo ; Li, Jiaoyong ; Su, Guoxun ; Feng, Baoyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a8fb99c6b18838dec78e05fed83160ff0a45a47b3aad373a2eedff038670dc0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aromatic hydrocarbons</topic><topic>Bacteria</topic><topic>Base Composition</topic><topic>Benzene Derivatives - metabolism</topic><topic>Biodegradation, Environmental</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>China</topic><topic>Chromosomes</topic><topic>Culture Media - chemistry</topic><topic>Degradation</topic><topic>Enterobacteriaceae - genetics</topic><topic>Enterobacteriaceae - metabolism</topic><topic>Ethylbenzene</topic><topic>Gene sequencing</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Hydrocarbon-degrading bacteria</topic><topic>Hydrocarbons</topic><topic>Hydrocarbons, Aromatic - metabolism</topic><topic>Iron</topic><topic>Kosakonia radicincitans</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molybdenum</topic><topic>Nitrogen</topic><topic>Nitrogen Fixation</topic><topic>Nitrogenase</topic><topic>Nitrogenase - metabolism</topic><topic>Nitrogenation</topic><topic>Nucleotide sequence</topic><topic>Physical characteristics</topic><topic>Plant Roots - microbiology</topic><topic>Pollutants</topic><topic>Reverse transcription</topic><topic>Sequence Analysis, DNA</topic><topic>Sustainable agriculture</topic><topic>Toluene</topic><topic>Toluene - metabolism</topic><topic>Whole Genome Sequencing</topic><topic>Xylene</topic><topic>Xylenes - metabolism</topic><topic>Zea mays</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yunpeng</creatorcontrib><creatorcontrib>Huang, Zhibo</creatorcontrib><creatorcontrib>Li, Jiaoyong</creatorcontrib><creatorcontrib>Su, Guoxun</creatorcontrib><creatorcontrib>Feng, Baoyun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yunpeng</au><au>Huang, Zhibo</au><au>Li, Jiaoyong</au><au>Su, Guoxun</au><au>Feng, Baoyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete Genome Sequence of Kosakonia radicincitans GXGL-4A, a Nitrogen-Fixing Bacterium with Capability to Degrade TEX</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>77</volume><issue>8</issue><spage>1848</spage><epage>1857</epage><pages>1848-1857</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Kosakonia radicincitans
GXGL-4A, a free-living nitrogen-fixing (NF) bacterial strain isolated from maize (
Zea mays
L.) roots was found to have ability to degrade aromatic hydrocarbons. In this study, we describe the main morphological characteristics of bacterium, aromatic hydrocarbon-degrading capability, and the complete genome of
K
.
radicincitans
GXGL-4A. The genome is consisted of only one 5,687,681 bp linear chromosome with a G + C content of 53.96%. The strain has two genetically distinct nitrogenase systems, one based on molybdenum (Mo) similar to nitrogenase isolated from a wide range of nitrogen-fixing organisms, and the other contains iron (Fe). The differences in transcriptional level of several important nitrogen fixation (
nif
) genes between LB (nitrogen-rich, NR) and A15 nitrogen-free (nitrogen-limited, NL) culture conditions were detected using Real-time Quantitative Reverse Transcription PCR (qRT-PCR). The bacterial cells of GXGL-4A can grow well in LB liquid medium containing 1% toluene, ethylbenzene or xylene, suggesting a good resistance to the tested aromatic hydrocarbons. The results of GC–MS analysis showed that
K
.
radicincitans
GXGL-4A has a good capability to degrade toluene, ethylbenzene, and xylene (TEX). Completion of the genome sequencing will no doubt contribute to the deep exploration and comprehensive utilization of this NF bacterium in sustainable agriculture and bioremediation of aromatic pollutants.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32170407</pmid><doi>10.1007/s00284-020-01942-3</doi><tpages>10</tpages></addata></record> |
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subjects | Aromatic hydrocarbons Bacteria Base Composition Benzene Derivatives - metabolism Biodegradation, Environmental Biomedical and Life Sciences Bioremediation Biotechnology Cell culture China Chromosomes Culture Media - chemistry Degradation Enterobacteriaceae - genetics Enterobacteriaceae - metabolism Ethylbenzene Gene sequencing Genome, Bacterial Genomes Hydrocarbon-degrading bacteria Hydrocarbons Hydrocarbons, Aromatic - metabolism Iron Kosakonia radicincitans Life Sciences Microbiology Molybdenum Nitrogen Nitrogen Fixation Nitrogenase Nitrogenase - metabolism Nitrogenation Nucleotide sequence Physical characteristics Plant Roots - microbiology Pollutants Reverse transcription Sequence Analysis, DNA Sustainable agriculture Toluene Toluene - metabolism Whole Genome Sequencing Xylene Xylenes - metabolism Zea mays Zea mays - microbiology |
title | Complete Genome Sequence of Kosakonia radicincitans GXGL-4A, a Nitrogen-Fixing Bacterium with Capability to Degrade TEX |
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