Investigation of the Effects of Octreotide Agent on Oxidative Stress, 8-Hydroxy Deoxyguanosine in Experimental Hepatic Carcinogenesis Rat Model
2-AAF and DEN are well-known liver toxicants commonly used to stimulate tumors in laboratory animals. The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats. In this study, 64 Wistar albino rats were divided...
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| Veröffentlicht in: | Folia Medica 2020-03, Vol.62 (1), p.70-75 |
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| description | 2-AAF and DEN are well-known liver toxicants commonly used to stimulate tumors in laboratory animals.
The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats.
In this study, 64 Wistar albino rats were divided into 8 groups. DEN (175 mg/kg) initiated and 2-AAF (20 mg/kg) promoted liver carcinogenesis in rats. The tumor growth inhibitor octreotide (300 μg/kg) was used. Rats were sacrificed at the end of experiment and their liver tissues were taken for the study. SOD, GSH-Px, CAT activities, NO and MDA levels were measured spectrophotometrically. Also, Hsp70 and 8-OHdG was measured by the ELISA method.
In group 7, MDA, 8-OHdG, and Hsp70 levels were significantly increased. In addition, SOD, GSH-Px activity was significantly reduced in this group. MDA, 8-OHdG and Hsp70 levels were significantly reduced in Group 8, which received octreotide for treatment.
DEN and 2-AAF cause very serious liver damage. Octreotide protects the liver from carcinogenesis, increases the activity of cellular antioxidant enzymes and helps reduce DNA damage. Therefore, octreotide may be an inhibitor in tumor cells and may reduce oxidative stress. |
| doi_str_mv | 10.3897/folmed.62.e47735 |
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The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats.
In this study, 64 Wistar albino rats were divided into 8 groups. DEN (175 mg/kg) initiated and 2-AAF (20 mg/kg) promoted liver carcinogenesis in rats. The tumor growth inhibitor octreotide (300 μg/kg) was used. Rats were sacrificed at the end of experiment and their liver tissues were taken for the study. SOD, GSH-Px, CAT activities, NO and MDA levels were measured spectrophotometrically. Also, Hsp70 and 8-OHdG was measured by the ELISA method.
In group 7, MDA, 8-OHdG, and Hsp70 levels were significantly increased. In addition, SOD, GSH-Px activity was significantly reduced in this group. MDA, 8-OHdG and Hsp70 levels were significantly reduced in Group 8, which received octreotide for treatment.
DEN and 2-AAF cause very serious liver damage. Octreotide protects the liver from carcinogenesis, increases the activity of cellular antioxidant enzymes and helps reduce DNA damage. Therefore, octreotide may be an inhibitor in tumor cells and may reduce oxidative stress.</description><identifier>ISSN: 0204-8043</identifier><identifier>EISSN: 1314-2143</identifier><identifier>DOI: 10.3897/folmed.62.e47735</identifier><identifier>PMID: 32337899</identifier><language>eng</language><publisher>Bulgaria: MEDICAL UNIVERSITY- PLOVDIV</publisher><subject>2-AAF ; 2-Acetylaminofluorene - toxicity ; 8-Hydroxy-2'-Deoxyguanosine - metabolism ; Animals ; Antineoplastic Agents, Hormonal - pharmacology ; Antioxidants ; Carcinogens - toxicity ; Carcinoma, Hepatocellular - chemically induced ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Catalase - drug effects ; Catalase - metabolism ; DEN ; Deoxyribonucleic acid ; Diethylnitrosamine - toxicity ; DNA ; DNA damage ; Glutathione Peroxidase - drug effects ; Glutathione Peroxidase - metabolism ; Heat shock proteins ; Hsp70 ; HSP70 Heat-Shock Proteins ; Laboratory animals ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Lipids ; Liver - drug effects ; Liver - metabolism ; Liver - pathology ; Liver cancer ; Liver Neoplasms, Experimental - chemically induced ; Liver Neoplasms, Experimental - metabolism ; Liver Neoplasms, Experimental - pathology ; Malondialdehyde - metabolism ; Methods ; Nitric Oxide - metabolism ; octreotide ; Octreotide - pharmacology ; Oxidative stress ; Oxidative Stress - drug effects ; Rats ; Rats, Wistar ; Statistical analysis ; Studies ; Superoxide Dismutase - drug effects ; Superoxide Dismutase - metabolism ; Tumors</subject><ispartof>Folia Medica, 2020-03, Vol.62 (1), p.70-75</ispartof><rights>This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>Copyright MEDICAL UNIVERSITY- PLOVDIV 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-1415-0563</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32337899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Unsal, Velid</creatorcontrib><creatorcontrib>Kurutaş, Ergül Belge</creatorcontrib><title>Investigation of the Effects of Octreotide Agent on Oxidative Stress, 8-Hydroxy Deoxyguanosine in Experimental Hepatic Carcinogenesis Rat Model</title><title>Folia Medica</title><addtitle>Folia Med (Plovdiv)</addtitle><description>2-AAF and DEN are well-known liver toxicants commonly used to stimulate tumors in laboratory animals.
The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats.
In this study, 64 Wistar albino rats were divided into 8 groups. DEN (175 mg/kg) initiated and 2-AAF (20 mg/kg) promoted liver carcinogenesis in rats. The tumor growth inhibitor octreotide (300 μg/kg) was used. Rats were sacrificed at the end of experiment and their liver tissues were taken for the study. SOD, GSH-Px, CAT activities, NO and MDA levels were measured spectrophotometrically. Also, Hsp70 and 8-OHdG was measured by the ELISA method.
In group 7, MDA, 8-OHdG, and Hsp70 levels were significantly increased. In addition, SOD, GSH-Px activity was significantly reduced in this group. MDA, 8-OHdG and Hsp70 levels were significantly reduced in Group 8, which received octreotide for treatment.
DEN and 2-AAF cause very serious liver damage. Octreotide protects the liver from carcinogenesis, increases the activity of cellular antioxidant enzymes and helps reduce DNA damage. Therefore, octreotide may be an inhibitor in tumor cells and may reduce oxidative stress.</description><subject>2-AAF</subject><subject>2-Acetylaminofluorene - toxicity</subject><subject>8-Hydroxy-2'-Deoxyguanosine - metabolism</subject><subject>Animals</subject><subject>Antineoplastic Agents, Hormonal - pharmacology</subject><subject>Antioxidants</subject><subject>Carcinogens - toxicity</subject><subject>Carcinoma, Hepatocellular - chemically induced</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Catalase - drug effects</subject><subject>Catalase - metabolism</subject><subject>DEN</subject><subject>Deoxyribonucleic acid</subject><subject>Diethylnitrosamine - toxicity</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Glutathione Peroxidase - drug effects</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Heat shock proteins</subject><subject>Hsp70</subject><subject>HSP70 Heat-Shock Proteins</subject><subject>Laboratory animals</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Lipids</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver cancer</subject><subject>Liver Neoplasms, Experimental - chemically induced</subject><subject>Liver Neoplasms, Experimental - metabolism</subject><subject>Liver Neoplasms, Experimental - pathology</subject><subject>Malondialdehyde - metabolism</subject><subject>Methods</subject><subject>Nitric Oxide - metabolism</subject><subject>octreotide</subject><subject>Octreotide - pharmacology</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Statistical analysis</subject><subject>Studies</subject><subject>Superoxide Dismutase - drug effects</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tumors</subject><issn>0204-8043</issn><issn>1314-2143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNo9kUtvEzEUhUcIRNPCnhWyxJYJ16_xzLIKoYlUFInH2vLY18FROg72pEp-BX8Zh2nZ2LLvOZ-PfKrqHYU5bzv1ycf9A7p5w-YolOLyRTWjnIqaUcFfVjNgIOoWBL-qrnPeATQSQL6urjjjXLVdN6v-rIdHzGPYmjHEgURPxl9Ilt6jHfPluLFjwjgGh-R2i8NIimpzCq7oH5F8L8OcP5K2Xp1diqcz-Yxl3R7NEHMYkISBLE8HTOGheM2erPBQnJYsTLJhiIWIOWTyzYzka3S4f1O98maf8e3TflP9_LL8sVjV95u79eL2vrYcuKx7J73vfd_xHhvb9kiNtJwy3jNmLe0sU-C9UQDOA_SgmhaANo4pCdj2lt9U64nrotnpQ8ln0llHE_S_i5i22qQSdI-aGQAFRjAphWAWDYjGOWWNkr1zrC2sDxPrkOLvY_lNvYvHNJT4mgnaKUlBqqKCSWVTzDmh__8qBX1pU09t6obpqc1ief8EPvaXybPhuT7-F-Emnlw</recordid><startdate>20200331</startdate><enddate>20200331</enddate><creator>Unsal, Velid</creator><creator>Kurutaş, Ergül Belge</creator><general>MEDICAL UNIVERSITY- PLOVDIV</general><general>Pensoft Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1415-0563</orcidid></search><sort><creationdate>20200331</creationdate><title>Investigation of the Effects of Octreotide Agent on Oxidative Stress, 8-Hydroxy Deoxyguanosine in Experimental Hepatic Carcinogenesis Rat Model</title><author>Unsal, Velid ; Kurutaş, Ergül Belge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3035-bd5ffbfb93be6c8be1a5c3123b22cc19c270ffa700df00b07680016d2750e8bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>2-AAF</topic><topic>2-Acetylaminofluorene - toxicity</topic><topic>8-Hydroxy-2'-Deoxyguanosine - metabolism</topic><topic>Animals</topic><topic>Antineoplastic Agents, Hormonal - pharmacology</topic><topic>Antioxidants</topic><topic>Carcinogens - toxicity</topic><topic>Carcinoma, Hepatocellular - chemically induced</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Catalase - drug effects</topic><topic>Catalase - metabolism</topic><topic>DEN</topic><topic>Deoxyribonucleic acid</topic><topic>Diethylnitrosamine - toxicity</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Glutathione Peroxidase - drug effects</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Heat shock proteins</topic><topic>Hsp70</topic><topic>HSP70 Heat-Shock Proteins</topic><topic>Laboratory animals</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Lipids</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver cancer</topic><topic>Liver Neoplasms, Experimental - chemically induced</topic><topic>Liver Neoplasms, Experimental - metabolism</topic><topic>Liver Neoplasms, Experimental - pathology</topic><topic>Malondialdehyde - metabolism</topic><topic>Methods</topic><topic>Nitric Oxide - metabolism</topic><topic>octreotide</topic><topic>Octreotide - pharmacology</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Statistical analysis</topic><topic>Studies</topic><topic>Superoxide Dismutase - drug effects</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Unsal, Velid</creatorcontrib><creatorcontrib>Kurutaş, Ergül Belge</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Folia Medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Unsal, Velid</au><au>Kurutaş, Ergül Belge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the Effects of Octreotide Agent on Oxidative Stress, 8-Hydroxy Deoxyguanosine in Experimental Hepatic Carcinogenesis Rat Model</atitle><jtitle>Folia Medica</jtitle><addtitle>Folia Med (Plovdiv)</addtitle><date>2020-03-31</date><risdate>2020</risdate><volume>62</volume><issue>1</issue><spage>70</spage><epage>75</epage><pages>70-75</pages><issn>0204-8043</issn><eissn>1314-2143</eissn><abstract>2-AAF and DEN are well-known liver toxicants commonly used to stimulate tumors in laboratory animals.
The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats.
In this study, 64 Wistar albino rats were divided into 8 groups. DEN (175 mg/kg) initiated and 2-AAF (20 mg/kg) promoted liver carcinogenesis in rats. The tumor growth inhibitor octreotide (300 μg/kg) was used. Rats were sacrificed at the end of experiment and their liver tissues were taken for the study. SOD, GSH-Px, CAT activities, NO and MDA levels were measured spectrophotometrically. Also, Hsp70 and 8-OHdG was measured by the ELISA method.
In group 7, MDA, 8-OHdG, and Hsp70 levels were significantly increased. In addition, SOD, GSH-Px activity was significantly reduced in this group. MDA, 8-OHdG and Hsp70 levels were significantly reduced in Group 8, which received octreotide for treatment.
DEN and 2-AAF cause very serious liver damage. Octreotide protects the liver from carcinogenesis, increases the activity of cellular antioxidant enzymes and helps reduce DNA damage. Therefore, octreotide may be an inhibitor in tumor cells and may reduce oxidative stress.</abstract><cop>Bulgaria</cop><pub>MEDICAL UNIVERSITY- PLOVDIV</pub><pmid>32337899</pmid><doi>10.3897/folmed.62.e47735</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-1415-0563</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 2-AAF 2-Acetylaminofluorene - toxicity 8-Hydroxy-2'-Deoxyguanosine - metabolism Animals Antineoplastic Agents, Hormonal - pharmacology Antioxidants Carcinogens - toxicity Carcinoma, Hepatocellular - chemically induced Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Catalase - drug effects Catalase - metabolism DEN Deoxyribonucleic acid Diethylnitrosamine - toxicity DNA DNA damage Glutathione Peroxidase - drug effects Glutathione Peroxidase - metabolism Heat shock proteins Hsp70 HSP70 Heat-Shock Proteins Laboratory animals Lipid peroxidation Lipid Peroxidation - drug effects Lipids Liver - drug effects Liver - metabolism Liver - pathology Liver cancer Liver Neoplasms, Experimental - chemically induced Liver Neoplasms, Experimental - metabolism Liver Neoplasms, Experimental - pathology Malondialdehyde - metabolism Methods Nitric Oxide - metabolism octreotide Octreotide - pharmacology Oxidative stress Oxidative Stress - drug effects Rats Rats, Wistar Statistical analysis Studies Superoxide Dismutase - drug effects Superoxide Dismutase - metabolism Tumors |
| title | Investigation of the Effects of Octreotide Agent on Oxidative Stress, 8-Hydroxy Deoxyguanosine in Experimental Hepatic Carcinogenesis Rat Model |
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