A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decay
EDC4 is a core component of processing (P)-bodies that binds the DCP2 decapping enzyme and stimulates mRNA decay. EDC4 also interacts with mammalian MARF1, a recently identified endoribonuclease that promotes oogenesis and contains a number of RNA binding domains, including two RRMs and multiple LOT...
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| description | EDC4 is a core component of processing (P)-bodies that binds the DCP2 decapping enzyme and stimulates mRNA decay. EDC4 also interacts with mammalian MARF1, a recently identified endoribonuclease that promotes oogenesis and contains a number of RNA binding domains, including two RRMs and multiple LOTUS domains. How EDC4 regulates MARF1 action and the identity of MARF1 target mRNAs is not known. Our transcriptome-wide analysis identifies bona fide MARF1 target mRNAs and indicates that MARF1 predominantly binds their 3' UTRs via its LOTUS domains to promote their decay. We also show that a MARF1 RRM plays an essential role in enhancing its endonuclease activity. Importantly, we establish that EDC4 impairs MARF1 activity by preventing its LOTUS domains from binding target mRNAs. Thus, EDC4 not only serves as an enhancer of mRNA turnover that binds DCP2, but also as a repressor that binds MARF1 to prevent the decay of MARF1 target mRNAs. |
| doi_str_mv | 10.7554/eLife.54995 |
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EDC4 also interacts with mammalian MARF1, a recently identified endoribonuclease that promotes oogenesis and contains a number of RNA binding domains, including two RRMs and multiple LOTUS domains. How EDC4 regulates MARF1 action and the identity of MARF1 target mRNAs is not known. Our transcriptome-wide analysis identifies bona fide MARF1 target mRNAs and indicates that MARF1 predominantly binds their 3' UTRs via its LOTUS domains to promote their decay. We also show that a MARF1 RRM plays an essential role in enhancing its endonuclease activity. Importantly, we establish that EDC4 impairs MARF1 activity by preventing its LOTUS domains from binding target mRNAs. Thus, EDC4 not only serves as an enhancer of mRNA turnover that binds DCP2, but also as a repressor that binds MARF1 to prevent the decay of MARF1 target mRNAs.</abstract><cop>CAMBRIDGE</cop><pub>Elife Sciences Publications Ltd</pub><pmid>32510323</pmid><doi>10.7554/eLife.54995</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0003-3700-7604</orcidid><orcidid>https://orcid.org/0000-0001-8962-7974</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biology Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Chromosomes and Gene Expression Endonuclease Endoribonucleases - chemistry Endoribonucleases - genetics Endoribonucleases - metabolism Experiments Gene expression HEK293 Cells Humans Life Sciences & Biomedicine Life Sciences & Biomedicine - Other Topics mRNA decapping mRNA decay mRNA turnover Oogenesis Proteins Proteins - chemistry Proteins - genetics Proteins - metabolism RNA binding protein RNA Caps - metabolism RNA Stability - genetics RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism Science & Technology Transcriptome - genetics |
| title | A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decay |
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