Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure
The excessive use of pesticides at different stages of crop production can pose a great danger to the aquatic environment, and particularly to fish. The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of m...
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| Veröffentlicht in: | Water (Basel) 2020-06, Vol.12 (6), p.1837 |
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| creator | Stoyanova, Stela Georgieva, Elenka Velcheva, Iliana Iliev, Ilia Vasileva, Tonka Bivolarski, Veselin Tomov, Stoil Nyeste, Krisztian Antal, Laszlo Yancheva, Vesela |
| description | The excessive use of pesticides at different stages of crop production can pose a great danger to the aquatic environment, and particularly to fish. The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of marker enzymes in common carp (Cyprinus carpio Linnaeus, 1758), by applying a multi-biomarker technique. The tested insecticide is categorized as a priority pollutant in surface waters in terms of Directive 2013/39/EU. The carps were exposed to different and environmentally relevant CPF concentrations for 72 h (a short-term acute experiment). The results showed that the tested insecticide alters the liver histological structure, causing degenerative lesions, such as granular and vacuolar degeneration; necrobiotic alterations and necrosis, as well as changes in the circulatory system. In addition, CPF induces changes in the enzymatic activity of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), cholinesterase (ChE), glutathione peroxidase (GPx) and catalase (CAT). The results from such experimental set ups could be successfully used in the legislation related to the protection of water bodies from contamination, in areas with intensive application of plant protection products used in agricultural practices, and also in implementing the Water Frame Directive by using multi-biomarker approaches. |
| doi_str_mv | 10.3390/w12061837 |
| format | Article |
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The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of marker enzymes in common carp (Cyprinus carpio Linnaeus, 1758), by applying a multi-biomarker technique. The tested insecticide is categorized as a priority pollutant in surface waters in terms of Directive 2013/39/EU. The carps were exposed to different and environmentally relevant CPF concentrations for 72 h (a short-term acute experiment). The results showed that the tested insecticide alters the liver histological structure, causing degenerative lesions, such as granular and vacuolar degeneration; necrobiotic alterations and necrosis, as well as changes in the circulatory system. In addition, CPF induces changes in the enzymatic activity of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), cholinesterase (ChE), glutathione peroxidase (GPx) and catalase (CAT). The results from such experimental set ups could be successfully used in the legislation related to the protection of water bodies from contamination, in areas with intensive application of plant protection products used in agricultural practices, and also in implementing the Water Frame Directive by using multi-biomarker approaches.</description><identifier>ISSN: 2073-4441</identifier><identifier>EISSN: 2073-4441</identifier><identifier>DOI: 10.3390/w12061837</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Agricultural practices ; Agricultural production ; Alanine ; Alanine transaminase ; Antioxidants ; Aquaculture ; Aquatic ecosystems ; Aquatic environment ; Aspartate aminotransferase ; Biological markers ; Biomarkers ; Carp ; Catalase ; Chemical contaminants ; Chemicals ; Chlorpyrifos ; Cholinesterase ; Circulatory system ; Contamination ; Crop production ; Cyprinus carpio ; Degeneration ; Dehydrogenases ; Drinking water ; Environmental aspects ; Enzymatic activity ; Enzymes ; Fish ; Fishing ; Glutathione peroxidase ; Health aspects ; Insecticides ; L-Lactate dehydrogenase ; Lactate dehydrogenase ; Lactic acid ; Legislation ; Liver ; Necrosis ; Oxidative stress ; Peroxidase ; Pesticides ; Physiology ; Plant protection ; Pollutants ; Surface water ; Toxicity ; Toxicology ; Water pollution</subject><ispartof>Water (Basel), 2020-06, Vol.12 (6), p.1837</ispartof><rights>COPYRIGHT 2020 MDPI AG</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-3b2da1c94fab36792f7a88f059fd9c6780bc74c17286f5d326d16aea0a6ecc553</citedby><cites>FETCH-LOGICAL-c359t-3b2da1c94fab36792f7a88f059fd9c6780bc74c17286f5d326d16aea0a6ecc553</cites><orcidid>0000-0001-9831-1429</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Stoyanova, Stela</creatorcontrib><creatorcontrib>Georgieva, Elenka</creatorcontrib><creatorcontrib>Velcheva, Iliana</creatorcontrib><creatorcontrib>Iliev, Ilia</creatorcontrib><creatorcontrib>Vasileva, Tonka</creatorcontrib><creatorcontrib>Bivolarski, Veselin</creatorcontrib><creatorcontrib>Tomov, Stoil</creatorcontrib><creatorcontrib>Nyeste, Krisztian</creatorcontrib><creatorcontrib>Antal, Laszlo</creatorcontrib><creatorcontrib>Yancheva, Vesela</creatorcontrib><title>Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure</title><title>Water (Basel)</title><description>The excessive use of pesticides at different stages of crop production can pose a great danger to the aquatic environment, and particularly to fish. The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of marker enzymes in common carp (Cyprinus carpio Linnaeus, 1758), by applying a multi-biomarker technique. The tested insecticide is categorized as a priority pollutant in surface waters in terms of Directive 2013/39/EU. The carps were exposed to different and environmentally relevant CPF concentrations for 72 h (a short-term acute experiment). The results showed that the tested insecticide alters the liver histological structure, causing degenerative lesions, such as granular and vacuolar degeneration; necrobiotic alterations and necrosis, as well as changes in the circulatory system. In addition, CPF induces changes in the enzymatic activity of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), cholinesterase (ChE), glutathione peroxidase (GPx) and catalase (CAT). The results from such experimental set ups could be successfully used in the legislation related to the protection of water bodies from contamination, in areas with intensive application of plant protection products used in agricultural practices, and also in implementing the Water Frame Directive by using multi-biomarker approaches.</description><subject>Agricultural practices</subject><subject>Agricultural production</subject><subject>Alanine</subject><subject>Alanine transaminase</subject><subject>Antioxidants</subject><subject>Aquaculture</subject><subject>Aquatic ecosystems</subject><subject>Aquatic environment</subject><subject>Aspartate aminotransferase</subject><subject>Biological markers</subject><subject>Biomarkers</subject><subject>Carp</subject><subject>Catalase</subject><subject>Chemical contaminants</subject><subject>Chemicals</subject><subject>Chlorpyrifos</subject><subject>Cholinesterase</subject><subject>Circulatory system</subject><subject>Contamination</subject><subject>Crop production</subject><subject>Cyprinus carpio</subject><subject>Degeneration</subject><subject>Dehydrogenases</subject><subject>Drinking water</subject><subject>Environmental aspects</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Fish</subject><subject>Fishing</subject><subject>Glutathione peroxidase</subject><subject>Health aspects</subject><subject>Insecticides</subject><subject>L-Lactate dehydrogenase</subject><subject>Lactate dehydrogenase</subject><subject>Lactic acid</subject><subject>Legislation</subject><subject>Liver</subject><subject>Necrosis</subject><subject>Oxidative stress</subject><subject>Peroxidase</subject><subject>Pesticides</subject><subject>Physiology</subject><subject>Plant protection</subject><subject>Pollutants</subject><subject>Surface water</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Water pollution</subject><issn>2073-4441</issn><issn>2073-4441</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptUU1LAzEQXUTBoj34DwJeLLg1X5uPY13qB1S86HlJs4mmdjdrsqv235tSkQrOHGbm8eYNvMmyMwSnhEh49YkwZEgQfpCNMOQkp5Siw73-OBvHuIIpqBSigKMsPAzr3uXXzjcqvJkAZjGaGBvT9sC1oPRN41NRoQMX5aYLrh0i0Gl0_hIsXNsqkwDECzFJ40cSULbfyuihN6B8XfvQbYKzPoL5V-fjEMxpdmTVOprxTz3Jnm_mT-Vdvni8vS9ni1yTQvY5WeJaIS2pVUvCuMSWKyEsLKStpWZcwKXmVCOOBbNFTTCrEVNGQcWM1kVBTrLznW4X_PtgYl-t_BDadLLCFAlZILzPelFrU7nW-j4o3bioqxmjjErOOUus6T-slLVpnPatsS7hfxYmuwUdfIzB2Cp5lyzeVAhW22dVv88i3xKrhL8</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Stoyanova, Stela</creator><creator>Georgieva, Elenka</creator><creator>Velcheva, Iliana</creator><creator>Iliev, Ilia</creator><creator>Vasileva, Tonka</creator><creator>Bivolarski, Veselin</creator><creator>Tomov, Stoil</creator><creator>Nyeste, Krisztian</creator><creator>Antal, Laszlo</creator><creator>Yancheva, Vesela</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-9831-1429</orcidid></search><sort><creationdate>20200601</creationdate><title>Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure</title><author>Stoyanova, Stela ; Georgieva, Elenka ; Velcheva, Iliana ; Iliev, Ilia ; Vasileva, Tonka ; Bivolarski, Veselin ; Tomov, Stoil ; Nyeste, Krisztian ; Antal, Laszlo ; Yancheva, Vesela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-3b2da1c94fab36792f7a88f059fd9c6780bc74c17286f5d326d16aea0a6ecc553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agricultural practices</topic><topic>Agricultural production</topic><topic>Alanine</topic><topic>Alanine transaminase</topic><topic>Antioxidants</topic><topic>Aquaculture</topic><topic>Aquatic ecosystems</topic><topic>Aquatic environment</topic><topic>Aspartate aminotransferase</topic><topic>Biological markers</topic><topic>Biomarkers</topic><topic>Carp</topic><topic>Catalase</topic><topic>Chemical contaminants</topic><topic>Chemicals</topic><topic>Chlorpyrifos</topic><topic>Cholinesterase</topic><topic>Circulatory system</topic><topic>Contamination</topic><topic>Crop production</topic><topic>Cyprinus carpio</topic><topic>Degeneration</topic><topic>Dehydrogenases</topic><topic>Drinking water</topic><topic>Environmental aspects</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Fish</topic><topic>Fishing</topic><topic>Glutathione peroxidase</topic><topic>Health aspects</topic><topic>Insecticides</topic><topic>L-Lactate dehydrogenase</topic><topic>Lactate dehydrogenase</topic><topic>Lactic acid</topic><topic>Legislation</topic><topic>Liver</topic><topic>Necrosis</topic><topic>Oxidative stress</topic><topic>Peroxidase</topic><topic>Pesticides</topic><topic>Physiology</topic><topic>Plant protection</topic><topic>Pollutants</topic><topic>Surface water</topic><topic>Toxicity</topic><topic>Toxicology</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stoyanova, Stela</creatorcontrib><creatorcontrib>Georgieva, Elenka</creatorcontrib><creatorcontrib>Velcheva, Iliana</creatorcontrib><creatorcontrib>Iliev, Ilia</creatorcontrib><creatorcontrib>Vasileva, Tonka</creatorcontrib><creatorcontrib>Bivolarski, Veselin</creatorcontrib><creatorcontrib>Tomov, Stoil</creatorcontrib><creatorcontrib>Nyeste, Krisztian</creatorcontrib><creatorcontrib>Antal, Laszlo</creatorcontrib><creatorcontrib>Yancheva, Vesela</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Water (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stoyanova, Stela</au><au>Georgieva, Elenka</au><au>Velcheva, Iliana</au><au>Iliev, Ilia</au><au>Vasileva, Tonka</au><au>Bivolarski, Veselin</au><au>Tomov, Stoil</au><au>Nyeste, Krisztian</au><au>Antal, Laszlo</au><au>Yancheva, Vesela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure</atitle><jtitle>Water (Basel)</jtitle><date>2020-06-01</date><risdate>2020</risdate><volume>12</volume><issue>6</issue><spage>1837</spage><pages>1837-</pages><issn>2073-4441</issn><eissn>2073-4441</eissn><abstract>The excessive use of pesticides at different stages of crop production can pose a great danger to the aquatic environment, and particularly to fish. The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of marker enzymes in common carp (Cyprinus carpio Linnaeus, 1758), by applying a multi-biomarker technique. The tested insecticide is categorized as a priority pollutant in surface waters in terms of Directive 2013/39/EU. The carps were exposed to different and environmentally relevant CPF concentrations for 72 h (a short-term acute experiment). The results showed that the tested insecticide alters the liver histological structure, causing degenerative lesions, such as granular and vacuolar degeneration; necrobiotic alterations and necrosis, as well as changes in the circulatory system. In addition, CPF induces changes in the enzymatic activity of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), cholinesterase (ChE), glutathione peroxidase (GPx) and catalase (CAT). The results from such experimental set ups could be successfully used in the legislation related to the protection of water bodies from contamination, in areas with intensive application of plant protection products used in agricultural practices, and also in implementing the Water Frame Directive by using multi-biomarker approaches.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/w12061837</doi><orcidid>https://orcid.org/0000-0001-9831-1429</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Agricultural practices Agricultural production Alanine Alanine transaminase Antioxidants Aquaculture Aquatic ecosystems Aquatic environment Aspartate aminotransferase Biological markers Biomarkers Carp Catalase Chemical contaminants Chemicals Chlorpyrifos Cholinesterase Circulatory system Contamination Crop production Cyprinus carpio Degeneration Dehydrogenases Drinking water Environmental aspects Enzymatic activity Enzymes Fish Fishing Glutathione peroxidase Health aspects Insecticides L-Lactate dehydrogenase Lactate dehydrogenase Lactic acid Legislation Liver Necrosis Oxidative stress Peroxidase Pesticides Physiology Plant protection Pollutants Surface water Toxicity Toxicology Water pollution |
| title | Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure |
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