Cell-free protein synthesis in hydrogel materials
We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthes...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2020-07, Vol.56 (52), p.718-7111 |
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| container_issue | 52 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Whitfield, Colette J Banks, Alice M Dura, Gema Love, John Fieldsend, Jonathan E Goodchild, Sarah A Fulton, David A Howard, Thomas P |
| description | We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.
Fabrication of macro-scale polysaccharide, proteinaceous, micellular and covalently crosslinked hydrogels for housing cell-free protein synthesis reactions. |
| doi_str_mv | 10.1039/d0cc02582h |
| format | Article |
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This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biocompatible Materials - chemistry Cell Extracts Cell Line Chemical synthesis Chemistry Chemistry, Multidisciplinary DNA - metabolism Hydrogels Hydrogels - chemistry Hydrogels - metabolism Liquid phases Physical Sciences Polyethylene Glycols - chemistry Polyethylene Glycols - metabolism Polymers - chemistry Polymers - metabolism Protein Biosynthesis - genetics Protein synthesis Proteins Proteins - genetics Science & Technology Sepharose - chemistry Sepharose - metabolism Tissue Scaffolds - chemistry |
| title | Cell-free protein synthesis in hydrogel materials |
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