Optimisation of PD-FcY veterinary antigen secretion from Nicotiana benthamiana hairy roots and purification from the culture medium

Hairy roots (HR) are a promising plant tissue-based platform for the production of biopharmaceuticals as the secretion of recombinant proteins in the culture medium may simplify the purification and recovery steps. In this study, Nicotiana benthamiana HR cultures were generated to produce the secret...

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Veröffentlicht in:	Plant cell, tissue and organ culture tissue and organ culture, 2020-07, Vol.142 (1), p.23-39
Hauptverfasser:	Rage, Emile, Marusic, Carla, Lico, Chiara, Salzano, Anna Maria, Scaloni, Andrea, Baschieri, Selene, Donini, Marcello
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-D Accumulation Antigens Biodegradation Biomedical and Life Sciences Dichlorophenoxyacetic acid Fluorescence Glycosylation Hairy root Infectious bursal disease Kinetics Life Sciences Nicotiana benthamiana Optimization Original Article Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plant tissues Protein purification Proteins Purification Recovery Red fluorescent protein Roots Secretion Ultrafiltration Vaccines Viruses
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