Near-Infrared Electrochemiluminescence Immunoassay with Biocompatible Au Nanoclusters as Tags
The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and envir...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-06, Vol.92 (11), p.7581-7587 |
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| creator | Yu, Lei Zhang, Qiao Kang, Qi Zhang, Bin Shen, Dazhong Zou, Guizheng |
| description | The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL
to 0.1 ng·mL
with a limit of detection of 1 fg mL
(S/N = 3) as well as desired selectivity. |
| doi_str_mv | 10.1021/acs.analchem.0c00125 |
| format | Article |
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to 0.1 ng·mL
with a limit of detection of 1 fg mL
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to 0.1 ng·mL
with a limit of detection of 1 fg mL
(S/N = 3) as well as desired selectivity.</description><subject>alpha-Fetoproteins - analysis</subject><subject>Analytical chemistry</subject><subject>Bioassays</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biosensing Techniques</subject><subject>Bovine serum albumin</subject><subject>Chemistry</subject><subject>Electrochemical Techniques</subject><subject>Electrochemiluminescence</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>I.R. radiation</subject><subject>Immunoassay</subject><subject>Immunosensors</subject><subject>Infrared Rays</subject><subject>Linearity</subject><subject>Luminescent Measurements</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Methionine</subject><subject>Multiplexing</subject><subject>Nanoclusters</subject><subject>Near infrared radiation</subject><subject>Selectivity</subject><subject>Serum albumin</subject><subject>Tags</subject><subject>Triethanolamine</subject><subject>α-Fetoprotein</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFPwjAYhhujEUT_gTFNPA-_tmsHRzSoJAQveDTNt7aTkW3Fdovh3zsCeHovz_scHkLuGYwZcPaEJo6xwcpsXD0GA8C4vCBDJjkkajLhl2QIACLhGcCA3MS47REGTF2TgeBCZRnjQ_K1chiSRVMEDM7SeeVMG_zBWVZdXTYuGtcYRxd13TUeY8Q9_S3bDX0uvfH1DtsyrxyddXSFjTdVF1sXIsVI1_gdb8lVgVV0d6cdkc_X-frlPVl-vC1eZsvECCHbpMimNisc4xZBAoe0EM4CT2WuDFgQgk-QT8HmXKGUwrJCMbQKHAieKiXEiDwevbvgfzoXW731XejjRM1TBhORTrnsqfRImeBjDK7Qu1DWGPaagT401X1TfW6qT03728NJ3uW1s_-nc0TxB2fodfk</recordid><startdate>20200602</startdate><enddate>20200602</enddate><creator>Yu, Lei</creator><creator>Zhang, Qiao</creator><creator>Kang, Qi</creator><creator>Zhang, Bin</creator><creator>Shen, Dazhong</creator><creator>Zou, Guizheng</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-1529-6356</orcidid><orcidid>https://orcid.org/0000-0002-3504-4893</orcidid><orcidid>https://orcid.org/0000-0003-2774-0458</orcidid><orcidid>https://orcid.org/0000-0002-3295-3848</orcidid></search><sort><creationdate>20200602</creationdate><title>Near-Infrared Electrochemiluminescence Immunoassay with Biocompatible Au Nanoclusters as Tags</title><author>Yu, Lei ; 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Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL
to 0.1 ng·mL
with a limit of detection of 1 fg mL
(S/N = 3) as well as desired selectivity.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32367712</pmid><doi>10.1021/acs.analchem.0c00125</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1529-6356</orcidid><orcidid>https://orcid.org/0000-0002-3504-4893</orcidid><orcidid>https://orcid.org/0000-0003-2774-0458</orcidid><orcidid>https://orcid.org/0000-0002-3295-3848</orcidid></addata></record> |
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| source | ACS Publications; MEDLINE |
| subjects | alpha-Fetoproteins - analysis Analytical chemistry Bioassays Biocompatibility Biocompatible Materials - chemistry Biosensing Techniques Bovine serum albumin Chemistry Electrochemical Techniques Electrochemiluminescence Gold - chemistry Humans I.R. radiation Immunoassay Immunosensors Infrared Rays Linearity Luminescent Measurements Metal Nanoparticles - chemistry Methionine Multiplexing Nanoclusters Near infrared radiation Selectivity Serum albumin Tags Triethanolamine α-Fetoprotein |
| title | Near-Infrared Electrochemiluminescence Immunoassay with Biocompatible Au Nanoclusters as Tags |
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