The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members
The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases (ω-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36 FAD genes, mapping to 16 of the species’ 20 chro...
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| Veröffentlicht in: | Plant molecular biology reporter 2020-06, Vol.38 (2), p.209-221 |
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| creator | Peng, Zhenying Ruan, Jian Tian, Haiying Shan, Lei Meng, Jingjing Guo, Feng Zhang, Zhimeng Ding, Hong Wan, Shubo Li, Xinguo |
| description | The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases (ω-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36
FAD
genes, mapping to 16 of the species’ 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (
SAD
),
FAD2
,
FAD3
,
FAD4/5
,
FAD6
and
FAD7/8
. Of these,
FAD3
and
FAD7/8
encoded ω-3 FADs, while genes belonging to the other four sub-families encoded ω-6 FADs. Based on RNA-Seq data, each of the 36
FAD
genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four
ω-3 AhFAD3
genes were functionally characterized; when expressed in
Arabidopsis thaliana
protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in
Arabidopsis thaliana
, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress. |
| doi_str_mv | 10.1007/s11105-019-01191-0 |
| format | Article |
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FAD
genes, mapping to 16 of the species’ 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (
SAD
),
FAD2
,
FAD3
,
FAD4/5
,
FAD6
and
FAD7/8
. Of these,
FAD3
and
FAD7/8
encoded ω-3 FADs, while genes belonging to the other four sub-families encoded ω-6 FADs. Based on RNA-Seq data, each of the 36
FAD
genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four
ω-3 AhFAD3
genes were functionally characterized; when expressed in
Arabidopsis thaliana
protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in
Arabidopsis thaliana
, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-019-01191-0</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acyl carrier protein ; Arabidopsis thaliana ; Bioinformatics ; Biomedical and Life Sciences ; Chromosomes ; Desaturase ; Endoplasmic reticulum ; Fads ; Fatty acids ; Functional analysis ; Gene mapping ; Genes ; Legumes ; Life Sciences ; Linolenic acid ; Mapping ; Metabolomics ; Organs ; Original Paper ; Peanut oil ; Peanuts ; Phylogeny ; Plant Breeding/Biotechnology ; Plant Sciences ; Proteomics ; Protoplasts ; Ribonucleic acid ; RNA ; Seedlings</subject><ispartof>Plant molecular biology reporter, 2020-06, Vol.38 (2), p.209-221</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-f4ccd0d3b546cc8ced14b172ccdb65ff6c7b0296cce363ac1528966448c7c4f53</citedby><cites>FETCH-LOGICAL-c363t-f4ccd0d3b546cc8ced14b172ccdb65ff6c7b0296cce363ac1528966448c7c4f53</cites><orcidid>0000-0003-1710-5127</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-019-01191-0$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-019-01191-0$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Peng, Zhenying</creatorcontrib><creatorcontrib>Ruan, Jian</creatorcontrib><creatorcontrib>Tian, Haiying</creatorcontrib><creatorcontrib>Shan, Lei</creatorcontrib><creatorcontrib>Meng, Jingjing</creatorcontrib><creatorcontrib>Guo, Feng</creatorcontrib><creatorcontrib>Zhang, Zhimeng</creatorcontrib><creatorcontrib>Ding, Hong</creatorcontrib><creatorcontrib>Wan, Shubo</creatorcontrib><creatorcontrib>Li, Xinguo</creatorcontrib><title>The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases (ω-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36
FAD
genes, mapping to 16 of the species’ 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (
SAD
),
FAD2
,
FAD3
,
FAD4/5
,
FAD6
and
FAD7/8
. Of these,
FAD3
and
FAD7/8
encoded ω-3 FADs, while genes belonging to the other four sub-families encoded ω-6 FADs. Based on RNA-Seq data, each of the 36
FAD
genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four
ω-3 AhFAD3
genes were functionally characterized; when expressed in
Arabidopsis thaliana
protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in
Arabidopsis thaliana
, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress.</description><subject>Acyl carrier protein</subject><subject>Arabidopsis thaliana</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Chromosomes</subject><subject>Desaturase</subject><subject>Endoplasmic reticulum</subject><subject>Fads</subject><subject>Fatty acids</subject><subject>Functional analysis</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Linolenic acid</subject><subject>Mapping</subject><subject>Metabolomics</subject><subject>Organs</subject><subject>Original Paper</subject><subject>Peanut oil</subject><subject>Peanuts</subject><subject>Phylogeny</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Proteomics</subject><subject>Protoplasts</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Seedlings</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kEFOwzAQRS0EEqVwAVaWWBvs2LGdZdSSggSCRVlbjmPTVG1SbGeRI3A6roRLkNixmBlp5r-v0QfgmuBbgrG4C4QQnCNMilSkIAifgBnJRYYKSeQpmGFBc1Rwhs_BRQhbnCAs5Qy49cbCSu_b3Qh7B1-t7oaYFjGOsDRtA5c26Dh4HSxc2c4GqLsGalgNnYlt3-kdLFMbQxuOfNUPHn59IgrLTVUuKXy2-9r6cAnOnN4Fe_U75-Ctul8vHtDTy-pxUT4hQzmNyDFjGtzQOmfcGGlsQ1hNRJa2Nc-d40bUOCvSzSa9NiTPZME5Y9IIw1xO5-Bm8j34_mOwIapt-ig9GFTGsJCEC54lVTapjO9D8Napg2_32o-KYHXMU015qpSn-slT4QTRCQpJ3L1b_2f9D_UN9lB3cw</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Peng, Zhenying</creator><creator>Ruan, Jian</creator><creator>Tian, Haiying</creator><creator>Shan, Lei</creator><creator>Meng, Jingjing</creator><creator>Guo, Feng</creator><creator>Zhang, Zhimeng</creator><creator>Ding, Hong</creator><creator>Wan, Shubo</creator><creator>Li, Xinguo</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-1710-5127</orcidid></search><sort><creationdate>20200601</creationdate><title>The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members</title><author>Peng, Zhenying ; Ruan, Jian ; Tian, Haiying ; Shan, Lei ; Meng, Jingjing ; Guo, Feng ; Zhang, Zhimeng ; Ding, Hong ; Wan, Shubo ; Li, Xinguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-f4ccd0d3b546cc8ced14b172ccdb65ff6c7b0296cce363ac1528966448c7c4f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acyl carrier protein</topic><topic>Arabidopsis thaliana</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Chromosomes</topic><topic>Desaturase</topic><topic>Endoplasmic reticulum</topic><topic>Fads</topic><topic>Fatty acids</topic><topic>Functional analysis</topic><topic>Gene mapping</topic><topic>Genes</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Linolenic acid</topic><topic>Mapping</topic><topic>Metabolomics</topic><topic>Organs</topic><topic>Original Paper</topic><topic>Peanut oil</topic><topic>Peanuts</topic><topic>Phylogeny</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Proteomics</topic><topic>Protoplasts</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Zhenying</creatorcontrib><creatorcontrib>Ruan, Jian</creatorcontrib><creatorcontrib>Tian, Haiying</creatorcontrib><creatorcontrib>Shan, Lei</creatorcontrib><creatorcontrib>Meng, Jingjing</creatorcontrib><creatorcontrib>Guo, Feng</creatorcontrib><creatorcontrib>Zhang, Zhimeng</creatorcontrib><creatorcontrib>Ding, Hong</creatorcontrib><creatorcontrib>Wan, Shubo</creatorcontrib><creatorcontrib>Li, Xinguo</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Zhenying</au><au>Ruan, Jian</au><au>Tian, Haiying</au><au>Shan, Lei</au><au>Meng, Jingjing</au><au>Guo, Feng</au><au>Zhang, Zhimeng</au><au>Ding, Hong</au><au>Wan, Shubo</au><au>Li, Xinguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2020-06-01</date><risdate>2020</risdate><volume>38</volume><issue>2</issue><spage>209</spage><epage>221</epage><pages>209-221</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases (ω-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36
FAD
genes, mapping to 16 of the species’ 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (
SAD
),
FAD2
,
FAD3
,
FAD4/5
,
FAD6
and
FAD7/8
. Of these,
FAD3
and
FAD7/8
encoded ω-3 FADs, while genes belonging to the other four sub-families encoded ω-6 FADs. Based on RNA-Seq data, each of the 36
FAD
genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four
ω-3 AhFAD3
genes were functionally characterized; when expressed in
Arabidopsis thaliana
protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in
Arabidopsis thaliana
, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11105-019-01191-0</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-1710-5127</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acyl carrier protein Arabidopsis thaliana Bioinformatics Biomedical and Life Sciences Chromosomes Desaturase Endoplasmic reticulum Fads Fatty acids Functional analysis Gene mapping Genes Legumes Life Sciences Linolenic acid Mapping Metabolomics Organs Original Paper Peanut oil Peanuts Phylogeny Plant Breeding/Biotechnology Plant Sciences Proteomics Protoplasts Ribonucleic acid RNA Seedlings |
| title | The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four ω-3 AhFAD3 Members |
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