Development and characterization of 16 microsatellite markers for the Louisiana pine snake, Pituophis ruthveni, and two congeners of conservation concern
We isolated and characterized 16 microsatellite loci from the Louisiana pine snake, Pituophis ruthveni . Loci were screened in 24 individuals from locations throughout its distribution in Louisiana and Texas. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0....
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| Veröffentlicht in: | Conservation genetics resources 2010-12, Vol.2 (1), p.163-166 |
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| creator | Kwiatkowski, Matthew A. Somers, Christopher M. Poulin, Ray G. Craig Rudolph, D. Martino, Jessica Tuberville, Tracey D. Hagen, Cris Lance, Stacey L. |
| description | We isolated and characterized 16 microsatellite loci from the Louisiana pine snake,
Pituophis ruthveni
. Loci were screened in 24 individuals from locations throughout its distribution in Louisiana and Texas. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity ranged from 0.043 to 0.298. We examined cross-species amplification at these loci in
P. catenifer
(bullsnakes and gopher snakes) and
P. melanoleucus
(pine snakes). These new markers provide tools for examining the conservation genetics of this species complex. Louisiana pine snakes face numerous threats: population densities are extremely low and their natural habitat has been severely altered and fragmented. In southern Canada,
P. catenifer
is at the northern extreme of its range and limited by the availability of suitable over-wintering sites. Hence, for these two species reduction of heterozygosity, potential for inbreeding, and increased effects of genetic drift are all of considerable conservation concern. |
| doi_str_mv | 10.1007/s12686-010-9208-3 |
| format | Article |
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Pituophis ruthveni
. Loci were screened in 24 individuals from locations throughout its distribution in Louisiana and Texas. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity ranged from 0.043 to 0.298. We examined cross-species amplification at these loci in
P. catenifer
(bullsnakes and gopher snakes) and
P. melanoleucus
(pine snakes). These new markers provide tools for examining the conservation genetics of this species complex. Louisiana pine snakes face numerous threats: population densities are extremely low and their natural habitat has been severely altered and fragmented. In southern Canada,
P. catenifer
is at the northern extreme of its range and limited by the availability of suitable over-wintering sites. Hence, for these two species reduction of heterozygosity, potential for inbreeding, and increased effects of genetic drift are all of considerable conservation concern.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-010-9208-3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Congeners ; Conservation Biology/Ecology ; Conservation genetics ; Ecology ; Evolutionary Biology ; Genetic drift ; Genetic markers ; Heterozygosity ; Inbreeding ; Life Sciences ; Microsatellites ; Pituophis catenifer ; Pituophis ruthveni ; Plant Genetics and Genomics ; Snakes ; Species ; Technical Note</subject><ispartof>Conservation genetics resources, 2010-12, Vol.2 (1), p.163-166</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Springer Science+Business Media B.V. 2010.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-b416b9271d02a293424b59747e7061892420f26591c54437cf02301cb8fa57613</citedby><cites>FETCH-LOGICAL-c359t-b416b9271d02a293424b59747e7061892420f26591c54437cf02301cb8fa57613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-010-9208-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-010-9208-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kwiatkowski, Matthew A.</creatorcontrib><creatorcontrib>Somers, Christopher M.</creatorcontrib><creatorcontrib>Poulin, Ray G.</creatorcontrib><creatorcontrib>Craig Rudolph, D.</creatorcontrib><creatorcontrib>Martino, Jessica</creatorcontrib><creatorcontrib>Tuberville, Tracey D.</creatorcontrib><creatorcontrib>Hagen, Cris</creatorcontrib><creatorcontrib>Lance, Stacey L.</creatorcontrib><title>Development and characterization of 16 microsatellite markers for the Louisiana pine snake, Pituophis ruthveni, and two congeners of conservation concern</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>We isolated and characterized 16 microsatellite loci from the Louisiana pine snake,
Pituophis ruthveni
. Loci were screened in 24 individuals from locations throughout its distribution in Louisiana and Texas. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity ranged from 0.043 to 0.298. We examined cross-species amplification at these loci in
P. catenifer
(bullsnakes and gopher snakes) and
P. melanoleucus
(pine snakes). These new markers provide tools for examining the conservation genetics of this species complex. Louisiana pine snakes face numerous threats: population densities are extremely low and their natural habitat has been severely altered and fragmented. In southern Canada,
P. catenifer
is at the northern extreme of its range and limited by the availability of suitable over-wintering sites. Hence, for these two species reduction of heterozygosity, potential for inbreeding, and increased effects of genetic drift are all of considerable conservation concern.</description><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Congeners</subject><subject>Conservation Biology/Ecology</subject><subject>Conservation genetics</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Genetic drift</subject><subject>Genetic markers</subject><subject>Heterozygosity</subject><subject>Inbreeding</subject><subject>Life Sciences</subject><subject>Microsatellites</subject><subject>Pituophis catenifer</subject><subject>Pituophis ruthveni</subject><subject>Plant Genetics and Genomics</subject><subject>Snakes</subject><subject>Species</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1UctOxCAUbYwm6ugHuCNxaxUohbI0vpNJdKFrwjC3DuMMVKBj9E_8W6k1unJ1703OI_ecojgi-JRgLM4iobzhJSa4lBQ3ZbVV7JFGiFJQjrd_95ruFvsxLjHmTUXpXvF5CRtY-W4NLiHt5sgsdNAmQbAfOlnvkG8R4WhtTfBRJ1itbAK01uEFQkStDygtAE19b6PVTqPOOkDR6Rc4QQ829b5b2IhCnxYbcPbk2yO9eWS8ewY3aGSDfEQIm9EwHwaCOyh2Wr2KcPgzJ8XT9dXjxW05vb-5uziflqaqZSpnjPCZpILMMdVUVoyyWS0FEyAwJ42kjOKW8loSUzNWCdNiWmFiZk2ra8FJNSmOR90u-NceYlJL3weXLRVlmMuGETmgyIgaYogBWtUFm1N4VwSroQE1NqByA2poQFWZQ0dOzNj8bfhT_p_0BZgTik4</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Kwiatkowski, Matthew A.</creator><creator>Somers, Christopher M.</creator><creator>Poulin, Ray G.</creator><creator>Craig Rudolph, D.</creator><creator>Martino, Jessica</creator><creator>Tuberville, Tracey D.</creator><creator>Hagen, Cris</creator><creator>Lance, Stacey L.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20101201</creationdate><title>Development and characterization of 16 microsatellite markers for the Louisiana pine snake, Pituophis ruthveni, and two congeners of conservation concern</title><author>Kwiatkowski, Matthew A. ; Somers, Christopher M. ; Poulin, Ray G. ; Craig Rudolph, D. ; Martino, Jessica ; Tuberville, Tracey D. ; Hagen, Cris ; Lance, Stacey L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-b416b9271d02a293424b59747e7061892420f26591c54437cf02301cb8fa57613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Congeners</topic><topic>Conservation Biology/Ecology</topic><topic>Conservation genetics</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Genetic drift</topic><topic>Genetic markers</topic><topic>Heterozygosity</topic><topic>Inbreeding</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>Pituophis catenifer</topic><topic>Pituophis ruthveni</topic><topic>Plant Genetics and Genomics</topic><topic>Snakes</topic><topic>Species</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwiatkowski, Matthew A.</creatorcontrib><creatorcontrib>Somers, Christopher M.</creatorcontrib><creatorcontrib>Poulin, Ray G.</creatorcontrib><creatorcontrib>Craig Rudolph, D.</creatorcontrib><creatorcontrib>Martino, Jessica</creatorcontrib><creatorcontrib>Tuberville, Tracey D.</creatorcontrib><creatorcontrib>Hagen, Cris</creatorcontrib><creatorcontrib>Lance, Stacey L.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwiatkowski, Matthew A.</au><au>Somers, Christopher M.</au><au>Poulin, Ray G.</au><au>Craig Rudolph, D.</au><au>Martino, Jessica</au><au>Tuberville, Tracey D.</au><au>Hagen, Cris</au><au>Lance, Stacey L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of 16 microsatellite markers for the Louisiana pine snake, Pituophis ruthveni, and two congeners of conservation concern</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2010-12-01</date><risdate>2010</risdate><volume>2</volume><issue>1</issue><spage>163</spage><epage>166</epage><pages>163-166</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>We isolated and characterized 16 microsatellite loci from the Louisiana pine snake,
Pituophis ruthveni
. Loci were screened in 24 individuals from locations throughout its distribution in Louisiana and Texas. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity ranged from 0.043 to 0.298. We examined cross-species amplification at these loci in
P. catenifer
(bullsnakes and gopher snakes) and
P. melanoleucus
(pine snakes). These new markers provide tools for examining the conservation genetics of this species complex. Louisiana pine snakes face numerous threats: population densities are extremely low and their natural habitat has been severely altered and fragmented. In southern Canada,
P. catenifer
is at the northern extreme of its range and limited by the availability of suitable over-wintering sites. Hence, for these two species reduction of heterozygosity, potential for inbreeding, and increased effects of genetic drift are all of considerable conservation concern.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-010-9208-3</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Congeners Conservation Biology/Ecology Conservation genetics Ecology Evolutionary Biology Genetic drift Genetic markers Heterozygosity Inbreeding Life Sciences Microsatellites Pituophis catenifer Pituophis ruthveni Plant Genetics and Genomics Snakes Species Technical Note |
| title | Development and characterization of 16 microsatellite markers for the Louisiana pine snake, Pituophis ruthveni, and two congeners of conservation concern |
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