Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata
Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. H...
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| Veröffentlicht in: | Conservation genetics resources 2010-12, Vol.2 (1), p.11-15 |
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| description | Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals:
Acropora cytherea
(
n
= 50),
Fungia scutaria
(
n
= 118),
Montipora capitata
(
n
= 140) and
Porites lobata
(
n
= 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and
Symbiodinium
endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsβ) for use in
M. capitata.
We report testing and optimization for seven of these microsatellites from
A. cytherea
, and eight microsatellites and the atpsβ locus from
M. capitata.
Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for
A. cytherea
and
M. capitata
, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae. |
| doi_str_mv | 10.1007/s12686-009-9118-4 |
| format | Article |
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Acropora cytherea
(
n
= 50),
Fungia scutaria
(
n
= 118),
Montipora capitata
(
n
= 140) and
Porites lobata
(
n
= 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and
Symbiodinium
endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsβ) for use in
M. capitata.
We report testing and optimization for seven of these microsatellites from
A. cytherea
, and eight microsatellites and the atpsβ locus from
M. capitata.
Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for
A. cytherea
and
M. capitata
, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-009-9118-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acropora cytherea ; Animal Genetics and Genomics ; Archipelagoes ; Biodiversity ; Biomedical and Life Sciences ; Conservation Biology/Ecology ; Corals ; Ecology ; Endosymbionts ; Evolutionary Biology ; Fungia scutaria ; Genetic markers ; Libraries ; Life Sciences ; Microsatellites ; Montipora capitata ; Plant Genetics and Genomics ; Population genetics ; Population studies ; Porites lobata ; Technical Note ; Zooxanthellae</subject><ispartof>Conservation genetics resources, 2010-12, Vol.2 (1), p.11-15</ispartof><rights>The Author(s) 2009</rights><rights>The Author(s) 2009. This work is published under https://creativecommons.org/licenses/by-nc/2.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-b8c4f0250960b06ff28eb7d055b1dea298e384beefa6ccdf5209c33fe902ecff3</citedby><cites>FETCH-LOGICAL-c359t-b8c4f0250960b06ff28eb7d055b1dea298e384beefa6ccdf5209c33fe902ecff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-009-9118-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-009-9118-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Concepcion, Gregory T.</creatorcontrib><creatorcontrib>Polato, Nicholas R.</creatorcontrib><creatorcontrib>Baums, Iliana B.</creatorcontrib><creatorcontrib>Toonen, Robert J.</creatorcontrib><title>Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals:
Acropora cytherea
(
n
= 50),
Fungia scutaria
(
n
= 118),
Montipora capitata
(
n
= 140) and
Porites lobata
(
n
= 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and
Symbiodinium
endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsβ) for use in
M. capitata.
We report testing and optimization for seven of these microsatellites from
A. cytherea
, and eight microsatellites and the atpsβ locus from
M. capitata.
Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for
A. cytherea
and
M. capitata
, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.</description><subject>Acropora cytherea</subject><subject>Animal Genetics and Genomics</subject><subject>Archipelagoes</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Corals</subject><subject>Ecology</subject><subject>Endosymbionts</subject><subject>Evolutionary Biology</subject><subject>Fungia scutaria</subject><subject>Genetic markers</subject><subject>Libraries</subject><subject>Life Sciences</subject><subject>Microsatellites</subject><subject>Montipora capitata</subject><subject>Plant Genetics and Genomics</subject><subject>Population genetics</subject><subject>Population studies</subject><subject>Porites lobata</subject><subject>Technical Note</subject><subject>Zooxanthellae</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM1OwzAQhCMEEqXwANwscSWwdv4cbhVQilQEBzhbjrsuLmkcbBfUV-CpcRUEJ047Ws3Mar8kOaVwQQGqS09ZycsUoE5rSnma7yUjyqsqrVgJ-7-6YIfJkfcrgJJnjI2Srxv8wNb2a-wCsZqsjXLWy4BtawKStXRv6DzRzq6JthtHZvJTGiM7oqyTrb8ikxjooyZqG17RoTwn0023NJJ4tQnSmbh4sF0wg0n2JsggiewW5Mm6eMST1jZxdZwc6NiIJz9znLxMb5-vZ-n88e7-ejJPVVbUIW24yjWwAuoSGii1ZhybagFF0dAFSlZzzHjeIGpZKrXQBYNaZZnGGhgqrbNxcjb09s6-b9AHsYqPdfGkYDmUNc-qjEUXHVw7Ht6hFr0zEcdWUBA75GJALiJysUMu8phhQ8ZHb7dE99f8f-gbKSOHkA</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Concepcion, Gregory T.</creator><creator>Polato, Nicholas R.</creator><creator>Baums, Iliana B.</creator><creator>Toonen, Robert J.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20101201</creationdate><title>Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata</title><author>Concepcion, Gregory T. ; Polato, Nicholas R. ; Baums, Iliana B. ; Toonen, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-b8c4f0250960b06ff28eb7d055b1dea298e384beefa6ccdf5209c33fe902ecff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acropora cytherea</topic><topic>Animal Genetics and Genomics</topic><topic>Archipelagoes</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation Biology/Ecology</topic><topic>Corals</topic><topic>Ecology</topic><topic>Endosymbionts</topic><topic>Evolutionary Biology</topic><topic>Fungia scutaria</topic><topic>Genetic markers</topic><topic>Libraries</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>Montipora capitata</topic><topic>Plant Genetics and Genomics</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Porites lobata</topic><topic>Technical Note</topic><topic>Zooxanthellae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Concepcion, Gregory T.</creatorcontrib><creatorcontrib>Polato, Nicholas R.</creatorcontrib><creatorcontrib>Baums, Iliana B.</creatorcontrib><creatorcontrib>Toonen, Robert J.</creatorcontrib><collection>Springer_OA刊</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Concepcion, Gregory T.</au><au>Polato, Nicholas R.</au><au>Baums, Iliana B.</au><au>Toonen, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2010-12-01</date><risdate>2010</risdate><volume>2</volume><issue>1</issue><spage>11</spage><epage>15</epage><pages>11-15</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals:
Acropora cytherea
(
n
= 50),
Fungia scutaria
(
n
= 118),
Montipora capitata
(
n
= 140) and
Porites lobata
(
n
= 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and
Symbiodinium
endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsβ) for use in
M. capitata.
We report testing and optimization for seven of these microsatellites from
A. cytherea
, and eight microsatellites and the atpsβ locus from
M. capitata.
Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for
A. cytherea
and
M. capitata
, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-009-9118-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Conservation genetics resources, 2010-12, Vol.2 (1), p.11-15 |
| issn | 1877-7252 1877-7260 |
| language | eng |
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| subjects | Acropora cytherea Animal Genetics and Genomics Archipelagoes Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Corals Ecology Endosymbionts Evolutionary Biology Fungia scutaria Genetic markers Libraries Life Sciences Microsatellites Montipora capitata Plant Genetics and Genomics Population genetics Population studies Porites lobata Technical Note Zooxanthellae |
| title | Development of microsatellite markers from four Hawaiian corals: Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata |
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