Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio
Fourteen microsatellite loci were isolated and characterized in the eastern elliptio ( Elliptio complanata ). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8...
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| Veröffentlicht in: | Conservation genetics resources 2010-12, Vol.2 (1), p.131-134 |
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| creator | Ward, Rocky Shaw, Kristine M. Small, Scott T. Lellis, William A. |
| description | Fourteen microsatellite loci were isolated and characterized in the eastern elliptio (
Elliptio complanata
). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8 to 20. Observed heterozygosity ranged from 0.350 to 0.900. Deviations from Hardy–Weinberg equilibrium were observed in five of 14 microsatellite loci. In each case, failure to meet Hardy–Weinberg expectation was due to heterozygote deficiency. Cross-species amplification was tested using four additional
Elliptio
species:
E. hoptenensis, E. shepardiana, E. dariensis,
and
E. icterina
. |
| doi_str_mv | 10.1007/s12686-010-9186-5 |
| format | Article |
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Elliptio complanata
). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8 to 20. Observed heterozygosity ranged from 0.350 to 0.900. Deviations from Hardy–Weinberg equilibrium were observed in five of 14 microsatellite loci. In each case, failure to meet Hardy–Weinberg expectation was due to heterozygote deficiency. Cross-species amplification was tested using four additional
Elliptio
species:
E. hoptenensis, E. shepardiana, E. dariensis,
and
E. icterina
.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-010-9186-5</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Conservation Biology/Ecology ; Ecology ; Elliptio ; Elliptio complanata ; Evolutionary Biology ; Heterozygosity ; Heterozygotes ; Life Sciences ; Plant Genetics and Genomics ; Rivers ; Species ; Technical Note</subject><ispartof>Conservation genetics resources, 2010-12, Vol.2 (1), p.131-134</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>Springer Science+Business Media B.V. 2010.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-56440462589c01816acb67db3c5b9d62f6fd18e300b935538793edbd7b1cc5f73</citedby><cites>FETCH-LOGICAL-c316t-56440462589c01816acb67db3c5b9d62f6fd18e300b935538793edbd7b1cc5f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-010-9186-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-010-9186-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Ward, Rocky</creatorcontrib><creatorcontrib>Shaw, Kristine M.</creatorcontrib><creatorcontrib>Small, Scott T.</creatorcontrib><creatorcontrib>Lellis, William A.</creatorcontrib><title>Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>Fourteen microsatellite loci were isolated and characterized in the eastern elliptio (
Elliptio complanata
). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8 to 20. Observed heterozygosity ranged from 0.350 to 0.900. Deviations from Hardy–Weinberg equilibrium were observed in five of 14 microsatellite loci. In each case, failure to meet Hardy–Weinberg expectation was due to heterozygote deficiency. Cross-species amplification was tested using four additional
Elliptio
species:
E. hoptenensis, E. shepardiana, E. dariensis,
and
E. icterina
.</description><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Elliptio</subject><subject>Elliptio complanata</subject><subject>Evolutionary Biology</subject><subject>Heterozygosity</subject><subject>Heterozygotes</subject><subject>Life Sciences</subject><subject>Plant Genetics and Genomics</subject><subject>Rivers</subject><subject>Species</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kctKxDAUhosoeH0AdwE3uqjmMklbd-IdFDfOOqTp6UyGNqlJqujD-KxmrOjKVU7g_77D4c-yQ4JPCcbFWSBUlCLHBOcVSQPfyHZIWRR5QQXe_J053c52Q1hhLEpG6U72eQWv0LmhBxuRsg3SS-WVjuDNh4rGWeRa1BvtXVARus5EQJ3TBhmLrtN3SBmkXT90yqqo0PGj67oxaHWO5jbhplFwMomTIuRhAG0gIJUI0xo97XgzcZmEcQloAXYMv-r9bKtVXYCDn3cvm99cP1_e5Q9Pt_eXFw-5ZkTEnIvZDM8E5WWlMSmJULoWRVMzzeuqEbQVbUNKYBjXFeOclUXFoKmboiZa87Zge9nR5B28exkhRLlyo7dppaQzLKqSMUFSikyp71s8tHLwplf-XRIs1zXIqQaZapDrGiRPDJ2YkLJ2Af7P_D_0BTx9jgc</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Ward, Rocky</creator><creator>Shaw, Kristine M.</creator><creator>Small, Scott T.</creator><creator>Lellis, William A.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20101201</creationdate><title>Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio</title><author>Ward, Rocky ; Shaw, Kristine M. ; Small, Scott T. ; Lellis, William A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-56440462589c01816acb67db3c5b9d62f6fd18e300b935538793edbd7b1cc5f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Elliptio</topic><topic>Elliptio complanata</topic><topic>Evolutionary Biology</topic><topic>Heterozygosity</topic><topic>Heterozygotes</topic><topic>Life Sciences</topic><topic>Plant Genetics and Genomics</topic><topic>Rivers</topic><topic>Species</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ward, Rocky</creatorcontrib><creatorcontrib>Shaw, Kristine M.</creatorcontrib><creatorcontrib>Small, Scott T.</creatorcontrib><creatorcontrib>Lellis, William A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ward, Rocky</au><au>Shaw, Kristine M.</au><au>Small, Scott T.</au><au>Lellis, William A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2010-12-01</date><risdate>2010</risdate><volume>2</volume><issue>1</issue><spage>131</spage><epage>134</epage><pages>131-134</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>Fourteen microsatellite loci were isolated and characterized in the eastern elliptio (
Elliptio complanata
). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8 to 20. Observed heterozygosity ranged from 0.350 to 0.900. Deviations from Hardy–Weinberg equilibrium were observed in five of 14 microsatellite loci. In each case, failure to meet Hardy–Weinberg expectation was due to heterozygote deficiency. Cross-species amplification was tested using four additional
Elliptio
species:
E. hoptenensis, E. shepardiana, E. dariensis,
and
E. icterina
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-010-9186-5</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1877-7252 |
| ispartof | Conservation genetics resources, 2010-12, Vol.2 (1), p.131-134 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Elliptio Elliptio complanata Evolutionary Biology Heterozygosity Heterozygotes Life Sciences Plant Genetics and Genomics Rivers Species Technical Note |
| title | Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio |
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