Epithelial mucosa as an alternative tissue for DNA extraction in amphibians

We evaluated the performance of amphibian epithelial mucosa as a non-destructive method for sampling DNA in four extraction protocols. We took tissue from 68 individuals of Eleutherodactylus johnstonei (Anura: Eleutherodactylidae) through a surface smear of each specimen with a sterile swab. DNA was...

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Veröffentlicht in:	Conservation genetics resources 2012-12, Vol.4 (4), p.1097-1099
Hauptverfasser:	Mendoza, Ángela María, García-Ramirez, Juan Carlos, Cárdenas-Henao, Heiber
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Chloroform Conservation Biology/Ecology Deoxyribonucleic acid DNA DNA sequencing Ecology Evolutionary Biology Life Sciences Mitochondria Mucosa Phenols Plant Genetics and Genomics Reptiles & amphibians rRNA 16S Technical Note
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