Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites
Many wild giraffe populations are declining across Africa, with two subspecies listed by the IUCN as Endangered in the past 4 years. We developed 11 microsatellite markers from Giraffa camelopardalis angolensis in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed...
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| Veröffentlicht in: | Conservation genetics resources 2012-12, Vol.4 (4), p.943-945 |
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| container_title | Conservation genetics resources |
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| creator | Carter, Kerryn D. Seddon, Jennifer M. Carter, John K. Goldizen, Anne W. Hereward, James P. |
| description | Many wild giraffe populations are declining across Africa, with two subspecies listed by the IUCN as Endangered in the past 4 years. We developed 11 microsatellite markers from
Giraffa camelopardalis angolensis
in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed 2–4 alleles per locus and expected heterozygosities of 0.082–0.711. There were no significant deviations from Hardy–Weinberg equilibrium for any of the loci. Null allele frequencies were low ( |
| doi_str_mv | 10.1007/s12686-012-9679-5 |
| format | Article |
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Giraffa camelopardalis angolensis
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Giraffa camelopardalis angolensis
in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed 2–4 alleles per locus and expected heterozygosities of 0.082–0.711. There were no significant deviations from Hardy–Weinberg equilibrium for any of the loci. Null allele frequencies were low (<3 %) across all loci. We present primer options for an additional 458 microsatellites. 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Seddon, Jennifer M. ; Carter, John K. ; Goldizen, Anne W. ; Hereward, James P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-f77dceaeba353c934bfe314de3b49c7bee1d4e08eaa547c83da114a3b1e4aa0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Gene frequency</topic><topic>Genetic markers</topic><topic>Giraffa camelopardalis</topic><topic>Giraffa camelopardalis angolensis</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>National parks</topic><topic>Plant Genetics and Genomics</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Quantitative genetics</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carter, Kerryn D.</creatorcontrib><creatorcontrib>Seddon, Jennifer M.</creatorcontrib><creatorcontrib>Carter, John K.</creatorcontrib><creatorcontrib>Goldizen, Anne W.</creatorcontrib><creatorcontrib>Hereward, James P.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carter, Kerryn D.</au><au>Seddon, Jennifer M.</au><au>Carter, John K.</au><au>Goldizen, Anne W.</au><au>Hereward, James P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2012-12-01</date><risdate>2012</risdate><volume>4</volume><issue>4</issue><spage>943</spage><epage>945</epage><pages>943-945</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>Many wild giraffe populations are declining across Africa, with two subspecies listed by the IUCN as Endangered in the past 4 years. 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Giraffa camelopardalis angolensis
in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed 2–4 alleles per locus and expected heterozygosities of 0.082–0.711. There were no significant deviations from Hardy–Weinberg equilibrium for any of the loci. Null allele frequencies were low (<3 %) across all loci. We present primer options for an additional 458 microsatellites. This new set of microsatellite markers and primer options will benefit conservation, population and quantitative genetics studies of giraffe populations.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-012-9679-5</doi><tpages>3</tpages></addata></record> |
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| ispartof | Conservation genetics resources, 2012-12, Vol.4 (4), p.943-945 |
| issn | 1877-7252 1877-7260 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Alleles Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Evolutionary Biology Gene frequency Genetic markers Giraffa camelopardalis Giraffa camelopardalis angolensis Life Sciences Microsatellites National parks Plant Genetics and Genomics Population genetics Population studies Quantitative genetics Technical Note |
| title | Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites |
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