Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples

A set of 14 microsatellite loci were used for amplification of faecal DNA from capercaillie ( Tetrao urogallus ). New internal primers were designed for each locus and were employed to perform a seminested PCR approach combined with a multiplex preamplification method. The aim of this process was to...

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Veröffentlicht in:Conservation genetics resources 2011-07, Vol.3 (3), p.483-487
Hauptverfasser: Pérez, Trinidad, Vázquez, José Fernando, Quirós, Francisco, Domínguez, Ana
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Sprache:eng
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