Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples
A set of 14 microsatellite loci were used for amplification of faecal DNA from capercaillie ( Tetrao urogallus ). New internal primers were designed for each locus and were employed to perform a seminested PCR approach combined with a multiplex preamplification method. The aim of this process was to...
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| Veröffentlicht in: | Conservation genetics resources 2011-07, Vol.3 (3), p.483-487 |
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| creator | Pérez, Trinidad Vázquez, José Fernando Quirós, Francisco Domínguez, Ana |
| description | A set of 14 microsatellite loci were used for amplification of faecal DNA from capercaillie (
Tetrao urogallus
). New internal primers were designed for each locus and were employed to perform a seminested PCR approach combined with a multiplex preamplification method. The aim of this process was to increase the quality of the multilocus genotypes and to reduce the amount of sample required. Values of allelic dropout and false alleles found after three repetitions were 8.1 and 1.4% respectively, and 52.63% of the samples amplified for a minimum of 12 loci. Additionally, capercaillie specific sex primers have been designed. These primers give short products suitable for degraded faecal sample amplification. New primers resulted in 87.5% of samples being successfully sexed, a value seven times higher than using the original P2/P8 CHD sexing method. |
| doi_str_mv | 10.1007/s12686-011-9385-8 |
| format | Article |
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Tetrao urogallus
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New primers resulted in 87.5% of samples being successfully sexed, a value seven times higher than using the original P2/P8 CHD sexing method.</description><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Life Sciences</subject><subject>Plant Genetics and Genomics</subject><subject>Primers</subject><subject>Sexing</subject><subject>Technical Note</subject><subject>Tetrao urogallus</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UU1LAzEQXUTBWv0B3gJe9LCaZLtJ1puIH4WCl3oOaXZ2Sdkma2Zb7H_xx5qloidPbxjeBzMvyy4ZvWWUyjtkXCiRU8byqlBlro6yCVNS5pILevw7l_w0O0NcUypUwfkk-5pv-hh2zrfEB587vzPodkBa8GHY9-PeeWJND9Ea13UOyPUShmgC2cbQmq7b4s09iVADutaPfITPEYyvycbZGNAMkIQDkD66DUQkQ0imNoJBINA0zjrwdk-CJ40BC0jQbPoO8Dw7aUyHcPGD0-z9-Wn5-Jov3l7mjw-L3BZMDHltRVVUwpZCQm1qs1pRKeSKQ8V5IwpVF021qhWnilalbNKPBOWCVbw0hteUF9Ps6uCbXvGxBRz0OmyjT5Gaz6io5EyyWWKxA2u8CSM0erzHxL1mVI8l6EMJOgXosQStkoYfNJi4voX45_y_6BuJoY2F</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Pérez, Trinidad</creator><creator>Vázquez, José Fernando</creator><creator>Quirós, Francisco</creator><creator>Domínguez, Ana</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20110701</creationdate><title>Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples</title><author>Pérez, Trinidad ; 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Tetrao urogallus
). New internal primers were designed for each locus and were employed to perform a seminested PCR approach combined with a multiplex preamplification method. The aim of this process was to increase the quality of the multilocus genotypes and to reduce the amount of sample required. Values of allelic dropout and false alleles found after three repetitions were 8.1 and 1.4% respectively, and 52.63% of the samples amplified for a minimum of 12 loci. Additionally, capercaillie specific sex primers have been designed. These primers give short products suitable for degraded faecal sample amplification. New primers resulted in 87.5% of samples being successfully sexed, a value seven times higher than using the original P2/P8 CHD sexing method.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-011-9385-8</doi><tpages>5</tpages></addata></record> |
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| ispartof | Conservation genetics resources, 2011-07, Vol.3 (3), p.483-487 |
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| language | eng |
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| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Evolutionary Biology Genotypes Genotyping Life Sciences Plant Genetics and Genomics Primers Sexing Technical Note Tetrao urogallus |
| title | Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples |
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